Adsorption of oligonucleotides onto negatively and positively charged latex particles

Elaı̈ssari, A.; Cros, Philippe; Pichot, Christian; Laurent, Victor; Mandrand, Bernard

doi:10.1016/0927-7757(93)02664-z

Cited by 41 publications

(27 citation statements)

References 9 publications

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“…In order to test the role of the charge of the graphene layer in the position and the orientation of the DNA, all of the carbon atoms of the graphene layer, to which DNA is anchored, were charged to one of the following values: À0.1, À0.05, À0.02, À0.01, 0, +0.01, +0.02, +0.05 or +0.1 elementary charge (e) per carbon, corresponding to the range of the charge densities À0.594 to +0.594 C m À2 comparable to those used in the experiments. 40,41 The sodium/chloride ions were used to neutralise fully the charge applied on the graphene, whereas the DNA was neutralised independently by sodium cations. All of the nominal charges per carbon atom, the charge densities and the number of ions used in the simulations are summarised in Table 1.…”

Section: A System Setupmentioning

confidence: 99%

Influence of a charged graphene surface on the orientation and conformation of covalently attached oligonucleotides: a molecular dynamics study

Kabeláč

Kroutil

Předota

et al. 2012

Phys. Chem. Chem. Phys.

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Molecular dynamics (MD) simulations of single-stranded (ss) and double-stranded (ds) oligonucleotides anchored via an aliphatic linker to a graphene surface were performed in order to investigate the role of the surface charge density in the structure and orientation of attached DNA. Two types of interactions of DNA with the surface are crucial for the stabilisation of the DNA-surface system. Whereas for a surface with a zero or low positive charge density the dispersion forces between the base(s) and the surface dominate, the higher charge densities applied on the surface lead to a strong electrostatic interaction between the phosphate groups of DNA, the surface and the ions. At high-charge densities, the interaction of the DNA with the surface is strongly affected by the formation of a low-mobility layer of counterions compensating for the charge of the surface. A considerable difference in the behaviour of the ds-DNA and ss-DNA anchored to the layer was observed. The ds-DNA interacts with the surface at low- and zero-charge densities exclusively by the nearest base pair. It keeps its geometry close to the canonical B-DNA form, even at surfaces with high-charge densities. The ss-DNA, owing to its much higher flexibility, has a tendency to maximise the attraction to the surface exploiting more bases for the interaction. The interaction of the polar amino group(s) of the base(s) of ss-DNA with a negatively charged surface also contributes significantly to the system stability.

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Section: A System Setupmentioning

confidence: 99%

Influence of a charged graphene surface on the orientation and conformation of covalently attached oligonucleotides: a molecular dynamics study

Kabeláč

Kroutil

Předota

et al. 2012

Phys. Chem. Chem. Phys.

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“…Synthetic single-stranded oligonucleotides (ODNs) fixed onto solid-phase supports are now widely used in assays based on the specific detection of complementary target DNA or RNA sequences contained in biological fluids. − Immobilization of nucleic acids onto solid supports can be performed through various methods such as, physical adsorption, covalent binding, or molecular recognition (biotin−streptavidin , system for instance); however, for preserving the hybridization properties of the probes, special attention should be paid to control the location and the conformation of the oligonucleotides at the interface after the immobilization step. Due to their polyelectrolyte properties, nucleic acids can interact with supports through different forces (electrostatic, hydrophobic, hydrogen bonding, etc.).…”

Section: Introductionmentioning

confidence: 99%

Effect of Triton X-405 on the Adsorption and Desorption of Single-Stranded DNA Fragments onto Positively Charged Latex Particles

1997

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The influence of a nonionic surfactant (Triton X-405) on the adsorption behavior of single-stranded oligodeoxyribonucleotide (poly(thymidylic acid) (dT35)) onto cationic aminated polystyrene latex particles has been investigated. A preliminary study was performed on the adsorption of Triton X-405 onto these cationic particles, showing a maximum amount of ∼0.5 mg m -2 at the plateau. Desorption of the nonionic surfactant even after an extensive cleaning procedure was not completed, indicating that probably low HLB (hydrophilic and lipophilic balance) species were still strongly adsorbed. The modification of the electrophoretic mobility of the latex particles together with the slight decrease in the colloidal stability suggested that the residual Triton X-405 molecules would adopt a flat conformation at the particles surface. Then, the adsorption behavior of dT 35 onto precoated cationic latex was thoroughly examined under various conditions and compared with the case of bare latex particles. It was clearly evidenced that the residual adsorbed amount of nonionic surfactant significantly affected the surface nature, especially at basic pH. It was interesting to notice that dT35 adsorption was strongly reduced at basic pH and that its desorption was favored using basic pH buffer containing Triton X-405 and high ionic strength. Kinetic exchange experiments were also performed using labeled 32 P-dT35 and unlabeled dT35, indicating that the exchange took place with no marked differences between coated and bare latex particles.

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“…The application of cationic polystyrene nanoparticles for adsorption studies of ONs has been described by Elaissari and coworkers [8,9]. Due to their biocompatiblity polystyrene nanoparticles are widely used as model drug carrier systems for in vitro and in vivo studies [10±12].…”

mentioning

confidence: 99%

Effects of oligonucleotide adsorption on the physicochemical characteristics of a nanoparticle-based model delivery system for antisense drugs

Götting¹,

Fritz²,

Maier³

et al. 1999

Colloid & Polymer Science

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Adsorption of oligonucleotides onto negatively and positively charged latex particles

Cited by 41 publications

References 9 publications

Influence of a charged graphene surface on the orientation and conformation of covalently attached oligonucleotides: a molecular dynamics study

Influence of a charged graphene surface on the orientation and conformation of covalently attached oligonucleotides: a molecular dynamics study

Effect of Triton X-405 on the Adsorption and Desorption of Single-Stranded DNA Fragments onto Positively Charged Latex Particles

Effects of oligonucleotide adsorption on the physicochemical characteristics of a nanoparticle-based model delivery system for antisense drugs

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