Adsorption of (Phe-h5)/(Phe-d5)-substituted peptides from neurotensin family on the nanostructured surfaces of Ag and Cu: SERS studies

Proniewicz, Leonard M.; Małuch, Izabela; Kudelski, Andrzej; Prahl, Adam

doi:10.1016/j.saa.2020.118748

Cited by 6 publications

(6 citation statements)

References 68 publications

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“…Peptide synthesis. Peptides were synthesized via the solid-phase method using the Fmoc/tBu strategy as previously described 49 with minor modifications and purified (see Supporting Information for details).…”

Section: Methodsmentioning

confidence: 99%

Spectroscopic characterization and in vitro studies of biological activity of bradykinin derivatives

Proniewicz

Burnat

Domin

et al. 2022

Sci Rep

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Eleven multiple analogs of bradykinin—a peptide that is a natural ligand of B1 and B2 receptors but does not bind or activate the B1 receptor unless Arg9 is removed from the sequence by the action of carboxypeptidase N—were synthesized. Their biological activity was examined on T-REx cell lines expressing B1 or B2 receptors using the intracellular IP1 assay. The mRNA expression of B1R and B2R in the lysate of tumor cell lines, e.g., U87-MG (human astrocytoma), SHP-77 (human small cell lung cancer), and H4 (human brain glioma), was determined. For five B1R antagonists, adsorption at the liquid/solid interface (Au nanoparticles (AuNPs) served as the solid surface) was discussed in terms of the vibrations of molecular fragments (structural factors) responsible for the biological properties of these analogs.

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Section: Methodsmentioning

confidence: 99%

Spectroscopic characterization and in vitro studies of biological activity of bradykinin derivatives

Proniewicz

Burnat

Domin

et al. 2022

Sci Rep

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“…Peptides synthesis. Peptides were synthesized via the solid-phase method using the Fmoc/tBu strategy as previously described 45 with minor modifications and purified (see Supporting Information for details).…”

Section: Methodsmentioning

confidence: 99%

Spectroscopic characterization and in vitro studies of biological activity of bradykinin derivatives

Proniewicz

Burnat

Domin

et al. 2022

Preprint

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Eleven multiple analogs of bradykinin (BK) – a peptide that is a natural ligand of B1 and B2 receptors – were synthesized. Their biological activity was evaluated on T-REx cell lines expressing B1 or B2 receptors using the intracellular IP1 assay. The mRNA expression of B1R and B2R in the lysate of tumor cell lines, e.g., U87-MG (human astrocytoma), SHP-77 (human small cell lung cancer), and H4 (human brain glioma), was determined. For five B1R antagonists, adsorption at the liquid/solid interface (Au nanoparticles (AuNPs) served as the solid surface) was discussed in terms of the vibrations of the molecular fragments (structural factors) responsible for the biological properties of these analogs.

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“…Peptides were synthesized via the solid-phase method using the Fmoc/ t Bu strategy, as previously described 74 with minor modifications. Briefly, TentaGel S RAM resin (Rapp Polymere, Germany) was used.…”

Section: Experimental Sectionmentioning

confidence: 99%

“…The biological studies reported in the literature also indicate that the active conformation of BN when interacting with GPCRs is a turn structure at positions 10−13 and hydrogen bonds between the amide NH 2 of L-methionine at position 14 of the amino acid sequence (Met 14 ) of BN and CO of Trp 8 , CO of Leu ■ EXPERIMENTAL SECTION Peptides. Peptides were synthesized via the solid-phase method using the Fmoc/tBu strategy, as previously described 74 with minor modifications. Briefly, TentaGel S RAM resin (Rapp Polymere, Germany) was used.…”

Section: ■ Conclusionmentioning

confidence: 99%

Application of Alanine Scanning to Determination of Amino Acids Essential for Peptide Adsorption at the Solid/Solution Interface and Binding to the Receptor: Surface-Enhanced Raman/Infrared Spectroscopy versus Bioactivity Assays

et al. 2021

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The article describes the application of the alanine-scanning technique used in combination with Raman, surface-enhanced Raman, attenuated total reflection Fourier transform infrared, and surface-enhanced infrared absorption (SEIRA) spectroscopies, which allowed defining the role of individual amino acid residues in the C -terminal 6–14 fragment of the bombesin chain (BN 6–14 ) on the path of its adsorption on the surface of Ag (AgNPs) and Au nanoparticles (AuNPs). A reliable analysis of the SEIRA spectra of these peptides was possible, thanks to a curve fitting of these spectra. By combining alanine-scanning with biological activity studies using cell lines overexpressing bombesin receptors and the intracellular inositol monophosphate assay, it was possible to determine which peptide side chains play a significant role in binding a peptide to membrane-bound G protein-coupled receptors (GPCRs). Based on the analysis of spectral profiles and bioactivity results, conclusions for the specific peptide–metal and peptide–GPCR interactions were drawn and compared.

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Adsorption of (Phe-h5)/(Phe-d5)-substituted peptides from neurotensin family on the nanostructured surfaces of Ag and Cu: SERS studies

Cited by 6 publications

References 68 publications

Spectroscopic characterization and in vitro studies of biological activity of bradykinin derivatives

Spectroscopic characterization and in vitro studies of biological activity of bradykinin derivatives

Spectroscopic characterization and in vitro studies of biological activity of bradykinin derivatives

Application of Alanine Scanning to Determination of Amino Acids Essential for Peptide Adsorption at the Solid/Solution Interface and Binding to the Receptor: Surface-Enhanced Raman/Infrared Spectroscopy versus Bioactivity Assays

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