Adsorption of proteins onto surfaces containing end-attached oligo(ethylene oxide): a model system using self-assembled monolayers

Prime, Kevin L.; Whitesides, George M.

doi:10.1021/ja00076a032

Cited by 1,567 publications

(1,607 citation statements)

References 5 publications

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“…These surfaces had previously resisted cell adhesion (Fig. 1), presumably through resisting protein adsorption [39,40]. However, adhesion studies on SAMs containing only EG alkanethiols (100% EG) with immobilized complexes or PEI alone demonstrate significant cell adhesion (Fig.…”

Section: Transfection On Sams On Goldmentioning

confidence: 93%

“…A SAM consisting of only EG groups (Fig. 1f) had no cells attached, which was expected due to the resistance of the surface to protein adsorption [39,40] that would be needed to support cellular adhesion. A complete elimination of cellular adhesion on SAMs containing 100% EG thiols indicates that the monolayers are robust.…”

Section: Cell Adhesion On Eg Samsmentioning

confidence: 94%

“…Additionally, free PEI in the complex solution, which would be more abundant at an N/P of 25, could be binding to the SAM and changing the surface properties, eliminating differences between surfaces. Although these EG-modified SAMs have substantially reduced protein adsorption [39,40], presumably through steric stabilization and excluded volume, some adsorption of serum proteins does occur [47,48], and complexes may bind to these surfaces via similar mechanisms.…”

Section: Quantification Of Complex Immobilizationmentioning

confidence: 99%

“…We hypothesize that, rather than attach PEG to the complexes directly, its presence in a SAM could enhance substrate-mediated transfection by conveying the desired properties of PEG on gene delivery (reduced complex aggregation, complex size stability), and promote interactions between the complexes and cell membrane. SAMs presenting oligo(ethylene glycol) (EG) groups [38,39] have previously been used to resist protein adsorption according to the length of the EG chain and its percent composition within the monolayer [39,40], and are used here to modulate DNA complex adsorption for substrate-mediated gene delivery. In our studies, EG-terminated alkanethiols were incorporated into SAMs at concentrations that do not limit cell adhesion and combinations of EG-and COO − -terminated alkanethiols were examined for their ability to bind and release complexes and to subsequently support transfection.…”

Section: Introductionmentioning

confidence: 99%

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Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

2008

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Substrate-mediated gene delivery describes the immobilization of gene therapy vectors to a biomaterial, which enhances gene transfer by exposing adhered cells to elevated DNA concentrations within the local microenvironment. Surface chemistry has been shown to affect transfection by nonspecifically immobilized complexes using self-assembled monolayers (SAMs) of alkanethiols on gold. In this report, SAMs were again used to provide a controlled surface to investigate whether the presence of oligo(ethylene glycol) (EG) groups in a SAM could affect complex morphology and enhance transfection. EG groups were included at percentages that did not affect cell adhesion. Nonspecific complex immobilization to SAMs containing combinations of EG-and carboxylic acidterminated alkanethiols resulted in substantially greater transfection than surfaces containing no EG groups or SAMs composed of EG groups combined with other functional groups. Enhancement in transfection levels could not be attributed to complex binding densities or release profiles. Atomic force microscopy imaging of immobilized complexes revealed that EG groups within SAMs affected complex size and appearance and could indicate the ability of these surfaces to preserve complex morphology upon binding. The ability to control the morphology of the immobilized complexes and influence transfection levels through surface chemistry could be translated to scaffolds for gene delivery in tissue engineering and diagnostic applications.

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Section: Transfection On Sams On Goldmentioning

confidence: 93%

Section: Cell Adhesion On Eg Samsmentioning

confidence: 94%

Section: Quantification Of Complex Immobilizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

2008

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“…sensors and biochips, 2,3 photovoltaics, 4,5 fuel cell, 6 biomaterials, 7 and molecular electronics, 4,8,9 as well as to provide fundamental understanding of electron transfer, 10 controlling reactions at surfaces, 11 and tailoring surface properties. 12 Among the many exciting developments, a number of rather sophisticated molecular assemblies have been developed that not only involve organic and organometallic molecules, but also nanomaterials and/or biomolecules.…”

Section: Simone Ciampimentioning

confidence: 99%

The molecular level modification of surfaces: from self-assembled monolayers to complex molecular assemblies

2011

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The modification of surfaces with self-assembled monolayers (SAMs) containing multiple different molecules, or containing molecules with multiple different functional components, or both, has become increasingly popular over the last two decades. This explosion of interest is primarily related to the ability to control the modification of interfaces with something approaching molecular level control and to the ability to characterise the molecular constructs by which the surface is modified. Over this time the level of sophistication of molecular constructs, and the level of knowledge related to how to fabricate molecular constructs on surfaces have advanced enormously. This critical review aims to guide researchers interested in modifying surfaces with a high degree of control to the use of organic layers. Highlighted are some of the issues to consider when working with SAMs, as well as some of the lessons learnt (169 references). 2011 The Royal Society of Chemistry. The modification of surfaces with self-assembled monolayers (SAMs) containing multiple different molecules, or containing molecules with multiple different functional components, or both, has become increasingly popular over the last two decades. This explosion of interest is primarily related to the ability to control the modification of interfaces with something approaching molecular level control and to the ability to characterise the molecular constructs by which the surface is modified. Over this time the level of sophistication of molecular constructs, and the level of knowledge related to how to fabricate molecular constructs on surfaces have advanced enormously. This critical review aims to guide researchers interested in modifying surfaces with a high degree of control to the use of organic layers. Highlighted are some of the issues to consider when working with SAMs, as well as some of the lessons learnt (169 references).

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Reduced protein adsorption on plastics via direct plasma deposition of triethylene glycol monoallyl ether

Beyer

Knoll

Ringsdorf

et al. 1997

J. Biomed. Mater. Res.

120

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The direct plasma-induced deposition of tri(ethylene glycol) monoallyl ether is reported. RF plasma polymerization of this monomer was carried out under both continuous wave (CW) and pulsed plasma operation. The major focus of this work was optimization of the degree of retention of the C-O-C bonds of the starting monomer during the deposition process. This successfully was accomplished using low RF power during the CW runs and low RF duty cycles during the pulsed plasma experiments. Spectroscopic analysis of the plasma films revealed a strong dependence of film composition on the RF power and duty cycles employed. In particular, an unusually high level of film chemistry compositional control was demonstrated for the pulsed plasma studies, with film composition varying in a steady, progressive fashion with sequential changes in the ratios of plasma on to plasma off times. This film chemistry controllability is demonstrated despite the relatively low volatility of the starting monomer. The utility of this plasma deposition approach in introducing polyethylene oxide (PEO) structures on solid substrates was evaluated via protein adsorption studies. Radiolabeled bovine albumin adsorption was studied on plasma-modified poly(ethylene teraphthalate) (PET) substrates. Dramatic reductions in both initial adsorption and retention of this protein were observed on PET samples having maximal PEO content relative to its adsorption on untreated PET surfaces. Good stability and adhesion of the plasma films to the underlying PET substrates were observed, as evidenced from prolonged immersion of plasma-treated surfaces in aqueous solution. Overall, the results obtained from the present work provide additional support for the utility of one-step plasma process to reduce biological fouling of surfaces via deposition of PEO surface units.

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Adsorption of proteins onto surfaces containing end-attached oligo(ethylene oxide): a model system using self-assembled monolayers

Cited by 1,567 publications

References 5 publications

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

The molecular level modification of surfaces: from self-assembled monolayers to complex molecular assemblies

Reduced protein adsorption on plastics via direct plasma deposition of triethylene glycol monoallyl ether

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