Adsorption of Sodium Dodecylbenzenesulphonate (SDBS) on Candida maltosa EH 15 Strain: Influence on Cell Surface Hydrophobicity and n-alkanes Biodegradation

Abstract

The effect of exogenously added sodium dodecylbenzenesulphonate (SDBS) surfactant on biodegradation of a mixture of straight-chain aliphatic hydrocarbons (dodecane and hexadecane) and resulting cell surface hydrophobicity changes of Candida maltosa EH 15 were investigated. Results indicated that up to 75 mg/L SDBS improves the biodegradation potential of examined yeast. A decrease in hydrophobicity was observed when SDBS was supplemented in higher concentrations, having strong impact on biodegradation rates. Phase distribution of surfactant molecules was investigated using methylene blue active substances method (MBAS), accompanied by surface and interfacial tension measurements. Studies showed that portion of SDBS molecules adsorbed on cell surface may play significant role in interaction between anionic surfactant and yeast cells, having influence on biodegradation rates.

Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil

“…Various gammaproteobacteria have the ability to produce biosurfactant molecules. For example, the Acinetobacter calcoaceticus RAG-1 can produce emulsan [47][48][49][50][51][52][53][54].…”

“…One of the reasons may be that the mechanisms responsible for biosurfactant excretion were controlled by the cells themselves, so that only necessary amounts of biosurfactant were produced when strains were growing on hexadecane. Although it has been possible to force microorganisms to overproduce biosurfactants such as rhamnolipids when cells have been cultivated on non-hydrophobic compounds like glycerol, such overproduction has never observed in the degradation of actual pollutants [50]. A further reason for the apparent lack of biosurfactant may be the decrease in pH.…”

Biodegradation of diesel fuel by a microbial consortium in the presence of 1-alkoxymethyl-2-methyl-5-hydroxypyridinium chloride homologues

“…Also for that reason, we do not attribute the cell-ILs interactions as a main reason for biodegradation decrease. Although modification of cell surface hydrophobicity may be altered in the presence of amphiphilic, surface active compounds, considering the cell densities used in biodegradation experiments and amounts of diesel fuel in the cultures, usually much higher amounts of surfactant are necessary to cause significant changes in cell surface hydrophobicity and/or degradation of hydrocarbons (Chrzanowski et al 2009). It is important to note that change in cell hydrophobicity is amid few parameters which may be modified by cells to minimize toxic effect exhibited by chemical compounds (Sikkema et al 1995;Isken and de Bont 1998).…”

Isolation and identification of the same type LMG1242 of biosurfactant-producing strain of Pseudomonas aeruginosa from different oil contaminated soils

“…It is known that the availability of particular compounds is a major rate limiting factor in biodegradation rate of oils rather than the chemical structure of the compounds (Sugiura et al, 1997). Also, previous research mainly indicates that biosurfactants are more effective than chemical surfactants in enhancing the solubility and biodegradation rate of petroleum hydrocarbons (Chrzanowski et al, 2008;Cybulski et al, 2003;Souza et al, 2014). Biosurfactants increase the bioavailability of hydrocarbons by emulsification of the oil phase resulting in enhanced bacterial growth and bioconversion.…”