Adsorption of Sr by immobilized microorganisms

Watson, J.S.; Scott, Charles D.; Faison, B. D.

doi:10.1007/bf02936518

Cited by 22 publications

(12 citation statements)

References 3 publications

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“…The high concentration of 137Cs in radioactive wastewaters from power reactors (Watson et al, 1989) indicates that aquatic ecosystems are especially susceptible to contamination with caesium. In aquatic environments, microalgae and cyanobacteria are important microbial primary producers and of these, Chlorella spp.…”

Section: Introductionmentioning

confidence: 99%

Replacement of cellular potassium by caesium in Chlorella emersonii: differential sensitivity of photoautotrophic and chemoheterotrophic growth

Avery

Codd

Gadd

1992

Journal of General Microbiology

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Photoautotrophic growth of Chforeffu emersonii in the presence of 1 mM-CsC1 resulted in a 34% increase in cell doubling time and an 83 % reduction in the final cell yield as compared to growth in the absence of Cs+. In contrast, the presence of 1 mM-Cs+ had no effect on chemoheterotrophic growth in the dark with glucose. These observations were correlated with the stage of growth at which Cs+-induced cellular K+ loss was evident. For photoautotrophically growing cells this occurred during the exponential growth phase (after 4 d); for chemoheterotrophically growing cells this was during stationary phase (after 10 d). Inhibition of chemoheterotrophic cell division occurred after 2 d in 50 mM-CS+ or 5 d in 20 mM-CS+, and coincided with a decline in intracellular K+ to -2 nmol (lo6 cells)-'. Accumulation of Cs+ ceased after 2 d in both of these cases. Cell doubling times during chemoheterotrophic growth remained approximately constant at internal K+ levels between 7 and 28 nmol (lo6 cells)-l. In contrast, a decline in intracellular K+, from 42 to 19 nmol (lo6 cells)-I , after 4 d photoautotrophic growth in the presence of Cs+, was concurrent with the 34% increase in cell doubling times. Photosynthesizing cells accumulated approximately 2-fold more Cs+ than respiring cells after incubation for 12 h in HEPES buffer, pH 8. Culture age and intracellular K+ levels had little effect on the ability of C. emersonii to accumulate Cs+. Externally supplied K+ inhibited Cs+ accumulation to a greater extent in photosynthesizing cells (75% inhibition at 10 mM-K+, 1 mM-Cs+) than in respiring cells (50% inhibition at 10 mM-K+, 1 mM-CS+). Greatly elevated Na+ concentrations (50 mM) were required to inhibit Cs+ accumulation in both cases. Incubation of cells in buffer in the light in the absence and presence of 10 mM-CS+ resulted in decreases in cellular K+ of 44% and 77% respectively and a concomitant 66% reduction in the rate of photosynthesis in both cases. A Cs+-induced K+ loss of 71 yo from respiring cells had no effect on the rate of respiration. These results indicate that it is not the presence of Cs+ in cells that is growth inhibitory, but rather the resulting loss of K+ and that photosynthesis and photoautotrophic growth are more sensitive to this K+ loss than respiration and chemoheterotrophic growth.

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Section: Introductionmentioning

confidence: 99%

Replacement of cellular potassium by caesium in Chlorella emersonii: differential sensitivity of photoautotrophic and chemoheterotrophic growth

Avery

Codd

Gadd

1992

Journal of General Microbiology

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“…Of these, accumulating the metals onto biologically derived materials is one of the most promising (Macaskie and Dean, 1989). Previously, Watson et al (1989), have shown that bone gelatinbased gel beads are able to accumulate certain metal ions, such as strontium, and thus enhance the adsorption capacity of an immobilized biosorbent. Here, we quantify the adsorption capacity and the rate at which this process occurs in these beads for a specific metal, copper.…”

Section: Introductionmentioning

confidence: 99%

Accumulation of Cu++ onto modified bone-gelatin beads

Petersen¹,

Davison²,

Scott³

et al. 1990

Biotechnol Tech

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SummaryCu*+ adsorbs onto modified boric gelatin beads as a function of pH. T&c amount of Cu'+ adsorbed is similar to that which has been shown to be adsorb onto other biological materials including bacterial biomass. Langmuir adsorption isotherms were fitted to this data and both the asymptotic maximum solid-phase concentration and the equilibrium constant for these isotherms are presented as a function of the solution pH. The adsorption rate for a typical set of experimental conditions is presented. IntroductionHeavy metals, which can be toxic to living organisms, are present in many industrial aqueous waste streams. These metals may originate from a variety of sources, including the nuclear power, defense and fuel reprocessing industries, smelting and electroplating processes in the metal processing industry. and silk screening processes in the computer industry. In addition ground-waters at many sites are contaminated with these metal ions. making their removal and concentration a necessary step in the restoration of thcsc areas.Ideally, we would like to concentrate thcsc toxic metals into a form that can be transported easily. We would also prefer that the metal can be recovered easily in a relatively concentrated form so that it can be reused. Finally, we would prcfcr that the number of

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“…This fast metal removal from the solutions suggests that the main mechanism does not involve metabolic activity. Watson et al (1989) noted also for the biosorption of strontium a fast metal retention by M. luteus. Yang and Volesky (1999) used biomass from Sargassum to remove uranium from waste.…”

Section: Resultsmentioning

confidence: 96%

Bioadsorption and ion exchange of Cr3+ and Pb2+ solutions with algae

et al. 2009

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Heavy metals can be removed from effluents and recovered using physico-chemical mechanisms as biosorption processes. In this work "Arribada" seaweed biomass was employed to assess its biosorptive capacity for the chromium (Cr 3+ ) and lead (Pb 2+ ) cations that usually are present in waste waters of plating industries. Equilibrium and kinetic experiments were conducted in a mixed reactor on a batch basis. Biosorption equilibrium and fluidsolid mass transfer constants data were analyzed through the concept of ion exchange sorption isotherm. The respective equilibrium exchange constants (K eqCr = 173.42, K eqPb = 58.86) and volumetric mass transfer coefficients ((k mCr a) = 1.13 × 10 −3 s −1 , (k mPb a) = 0.89 × 10 −3 s −1 ) were employed for the dynamic analysis of Cr and Pb sorption in a fixed-bed flow-through sorption column. The breakthrough curves obtained for both metals were compared with the predicted values by the heterogeneous model (K eqCr = 171.29, K eqPb = 60.14; k mCr a = 7.81 × 10 −2 s −1 , k mPb a = 2.43 × 10 −2 s −1 ), taking into account the mass transfer process. The results suggest that these algae may be employed in a metal removal/recovery process at low cost.

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Adsorption of Sr by immobilized microorganisms

Cited by 22 publications

References 3 publications

Replacement of cellular potassium by caesium in Chlorella emersonii: differential sensitivity of photoautotrophic and chemoheterotrophic growth

Replacement of cellular potassium by caesium in Chlorella emersonii: differential sensitivity of photoautotrophic and chemoheterotrophic growth

Accumulation of Cu++ onto modified bone-gelatin beads

Bioadsorption and ion exchange of Cr3+ and Pb2+ solutions with algae

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