Adult follicular fluid supplementation during in vitro maturation improves the developmental competence of prepubertal lamb oocytes

Tian, Hao; Liu, Kexiong; Zhang, Yumei; Qi, Qi; Wang, Chunxin; Guan, Hong; Yan, Fengxiang; Hou, Jian

doi:10.1016/j.theriogenology.2019.03.009

Cited by 18 publications

(12 citation statements)

References 55 publications

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“…It is made up of secretions produced by peripheral granulosa cells and serum diffused by local capillaries, as well as plasma components that have crossed the blood follicular barrier, primarily hormones, growth factors, interleukins, anti-apoptosis factors, proteins, sugars, amino acids, active oxygen, and antioxidant enzymes [ 11 , 12 ]. It has an impact on oocyte maturation, follicular wall rupture, fertilization, and the development of early embryos [ 13 , 14 ]. Oxidative stress markers in FF are closely related to the growth, development, and maturation of oocytes.…”

Section: Introductionmentioning

confidence: 99%

UHPLC-MS-MS analysis of oxylipins metabolomics components of follicular fluid in infertile individuals with diminished ovarian reserve

Liang

Zhang

Qian

et al. 2021

Reprod Biol Endocrinol

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Background Diminished ovarian reserve (DOR) refers to a decrease in the number and quality of oocytes in the ovary, which results in a lack of sex hormones and a decline of fertility in women. DOR can potentially progress to premature ovarian failure (POF), which has a negative impact on women's quality of life and is a major cause of female infertility. Oxidative stress is a major contributor to fertility decrease in DOR patients, affecting the follicular microenvironment, oocyte maturation, fertilization, and embryo development. Understanding intracellular signal transduction can be achieved by defining specific oxidized lipid components in follicular fluid (FF) of DOR infertile patients. Methods The oxylipins metabolic signatures in the FF of DOR patients and females with normal ovarian reserve (NOR) enrolled for the in vitro fertilization (IVF) cycle were analyzed using UHPLC-MS-MS technology. Principal component analysis (PCA) and orthogonal projections to latent structure discriminant analysis (OPLS-DA) were used to analyze the derived metabolomic profiles. Pathway enrichment analysis was carried out using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and MetaboAnalyst databases. Furthermore, the Spearman rank correlation coefficient was used to determine the correlation between age, FSH, AMH, AFC, oocytes retrieved, MII oocytes, fertilization, high-quality embryos, and the concentration of differential oxidized lipid metabolites in FF. Results Fifteen oxylipins metabolites were found to be lower in the FF of DOR patients than those in the NOR group, including ±20-HDoHE, ±5-iso PGF2α-VI, 12S-HHTrE, 15-deoxy-Δ12,14-PGJ2, 1a,1b-dihomo PGE2, 1a,1b-dihomo PGF2α, 20-COOH-AA, 20-HETE, 8S,15S-DiHETE, PGA2, PGD2, PGE1, PGF1α, PGF2α, and PGJ2. The pathway enrichment analysis revealed that the 15 differentially oxidized lipid metabolites were closely related to the arachidonic acid metabolic pathway. Correlation analysis revealed that the concentration of 8 different oxidized lipid metabolites in FF was negatively correlated to FSH and positively correlated with AFC. AMH, the number of oocytes retrieved, MII oocytes and fertilization, were all positively correlated with 9 different oxidized lipid metabolites, but only one metabolite was positively correlated with the number of high-quality embryos. Conclusions Metabolomic analysis of FF revealed that oxylipins metabolism disorders were closely related to ovarian reserve function. Among these oxylipins metabolites, arachidonic acid metabolism undergoes significant changes that may be related to oocyte development, resulting in decreased fertility in DOR patients. Trial registration ChiCTR, ChiCTR2000038182, Registered 12 September 2020-Retrospectively registered

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Section: Introductionmentioning

confidence: 99%

UHPLC-MS-MS analysis of oxylipins metabolomics components of follicular fluid in infertile individuals with diminished ovarian reserve

Liang

Zhang

Qian

et al. 2021

Reprod Biol Endocrinol

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“…Nevertheless, embryos derived from JIVET exhibit a significantly lower survival rate [38]. The maturation status of the cumulus cell [39], oocyte cytoplasm [40], oocyte nuclear [41], and follicular fluid microenvironment in juvenile ovaries [42][43][44] have been suggested to explain the poor efficiency of JIVET. In the present study, we focused on the initial stage of JIVET: ovaries under the treatment of superovulation; therefore, the miRNAome and transcriptome were compared between juvenile and adult ovaries to identify the mechanism of inferior oocyte capture by JIVET.…”

Section: Discussionmentioning

confidence: 99%

The Roles of the miRNAome and Transcriptome in the Ovine Ovary Reveal Poor Efficiency in Juvenile Superovulation

Zhang¹,

Dong²,

Yang³

et al. 2021

Animals

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Juvenile superovulation can provide a wealth of oocyte material for embryo production, animal cloning, and genetic modification research, but embryos derived from juvenile oocytes show poor efficiency in subsequent developmental capacity. In order to reveal the formation mechanism of large numbers of follicles and poor oocyte quality in juvenile ovaries under superovulation treatment, differentially expressed microRNAs (miRNAs) and messenger RNAs (mRNAs) were characterized and investigated in the ovaries of lambs and adult sheep using high-throughput sequencing technology. The majority of differentially expressed miRNAs (337/358) were upregulated in lamb libraries. The expression levels of mRNAs related to hormone receptors (follicle-stimulating hormone receptor, FSHR; luteinizing hormone/choriogonadotropin receptor, LHCGR; estrogen receptor 1, ESR1), steroid hormone secretion (cytochrome P450 family 11 subfamily A member 1, CYP11A1; cytochrome P450 family 17 subfamily A member 1, CYP17A1; cytochrome P450 family 19 subfamily A member 1, CYP19A1), and oocyte quality (pentraxin 3, PTX3; BCL2 apoptosis regulator, BCL2; caspase 3, CASP3) were significantly different between the lamb and adult libraries. The miRNA aor-miR-143, which targets FSHR, was highly and differentially expressed, and PTX3 was predicted to be targeted by oar-miR-485-3p and oar-miR-377-3p in the ovine ovary. A considerable number of miRNAs were predicted to inhibit ESR1 expression in lamb ovaries. In conclusion, oar-miR-143 and FSHR molecules, among others, might regulate follicle formation, and oar-miR-485-3p, oar-miR-377-3p, and PTX3, among others, may be associated with oocyte quality. These identified miRNAs and mRNAs will be beneficial for the prediction of ovarian superovulation potential and screening of oocytes.

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“…Oocytes were recovered from lambs and ewes approximately 12 h after the last FSH injection as described previously [14]. The animals were induced to general anesthesia by intramuscular injection of anesthetic and ovaries were exposed by midventral laparotomy.…”

Section: Animals Hormonal Treatment and Sample Collectionmentioning

confidence: 99%

“…The procedure of oocyte IVM/IVF was same as described previously [14]. Brie y, COCs were cultured at 38.5 C for 24 h in a humidi ed atmosphere of 5% CO 2 in TCM199 supplemented with 20% (v:v) estrous sheep serum (ESS), 10 µg/mL FSH (Folltropin-V; Bioniche Inc.,Belleville, Ont., Canada), 10 µg/mL LH (Bioniche Inc) and 1 µg/mL 17β-estradiol.…”

Section: Oocyte In Vitro Maturation (Ivm) and Fertilization (Ivf)mentioning

confidence: 99%

Transcriptomic comparison of ovarian granulosa cells between adult sheep and prepubertal lambs

Tian

Ren

Liu

et al. 2021

Preprint

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Background The oocyte development ability of prepubertal animals is significantly lower than that of adult animals. Granulosa cells (GCs) have an important function on regulation of follicular and oocyte development. Therefore, analysis of GCs characteristics can be used to explore the developmental mechanism of follicles and oocytes. Results In order to understand the possible reasons for the differences in follicles and oocytes development between lambs and adult sheep, we utilized high-throughput sequencing technique to analyze the transcriptome of GCs from follicle-stimulating hormone (FSH) superstimulated adult ewes and prepubertal lambs. Adult ewes were stimulated with FSH for 3 days (group A) and lambs were FSH-stimulated for 2 days (group B) or 3 days (group C). Transcriptome analysis of GCs showed that there were 405 and 159 differentially expressed genes from A vs. B and A vs. C, respectively. The results indicated that prolonging the FSH-stimulation of lambs made the GC state of lambs more similar to the adult sheep, but there were still a large number of differentially expressed genes between adult sheep and lambs. Further analysis showed that many differently expressed genes were implicated in cell proliferation and apoptosis, oocyte development and follicular ovulation. Cellular examination demonstrated that fatty acid binding protein 4 (FABP4), which was highly expressed in lamb GCs, had a potential of promoting cell apoptosis. Cytoplasmic phospholipase A2 (PLA2G4A), which was expressed lowly in lamb GCs, may be responsible for reduced synthesis of prostaglandins in cells and impaired follicle/oocyte development. In contrast, glutathione S-transferase β-1 (GSTT2B) and forkhead boxO6 (FOXO6) had no apparent effect on the proliferation and apoptosis of GCs.

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Adult follicular fluid supplementation during in vitro maturation improves the developmental competence of prepubertal lamb oocytes

Cited by 18 publications

References 55 publications

UHPLC-MS-MS analysis of oxylipins metabolomics components of follicular fluid in infertile individuals with diminished ovarian reserve

UHPLC-MS-MS analysis of oxylipins metabolomics components of follicular fluid in infertile individuals with diminished ovarian reserve

The Roles of the miRNAome and Transcriptome in the Ovine Ovary Reveal Poor Efficiency in Juvenile Superovulation

Transcriptomic comparison of ovarian granulosa cells between adult sheep and prepubertal lambs

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