Adult human colonic subepithelial myofibroblasts express extracellular matrix proteins and cyclooxygenase-1 and -2

Mahida, Yashwant R.; Beltinger, Johannes; Makh, S.; Göke, Michael; Gray, Trevor; Podolsky, Daniel K.; Hawkey, Christopher J.

doi:10.1152/ajpgi.1997.273.6.g1341

Cited by 110 publications

(143 citation statements)

References 25 publications

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“…Primary colonic SEMF cultures were prepared according to the method reported by Mahida et al [34]. The cellular characteristics and culture conditions have also been described in our previous report [35].…”

Section: Culture Of Human Colonic Subepithelial Myofibroblastsmentioning

confidence: 99%

The increased mucosal mRNA expressions of complement C3 and interleukin-17 in inflammatory bowel disease

Sugihara

Kobori

Imaeda

et al. 2010

Clinical and Experimental Immunology

110

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Section: Culture Of Human Colonic Subepithelial Myofibroblastsmentioning

confidence: 99%

The increased mucosal mRNA expressions of complement C3 and interleukin-17 in inflammatory bowel disease

Sugihara

Kobori

Imaeda

et al. 2010

Clinical and Experimental Immunology

110

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“…18Co was used in the study as a representative CMF. Additional primary cultures of subepithelial CMFs were generated according to the method described by Mahida et al (37,38). The purity of isolated cells was analyzed by immunohistochemistry and flow cytometry analysis as described below.…”

Section: Primary Cmf Culturesmentioning

confidence: 99%

Subepithelial Myofibroblasts are Novel Nonprofessional APCs in the Human Colonic Mucosa

Saada¹,

Pinchuk²,

Barrera³

et al. 2006

The Journal of Immunology

109

139

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The human gastrointestinal mucosa is exposed to a diverse normal microflora and dietary Ags and is a common site of entry for pathogens. The mucosal immune system must respond to these diverse signals with either the initiation of immunity or tolerance. APCs are important accessory cells that modulate T cell responses which initiate and maintain adaptive immunity. The ability of APCs to communicate with CD4+ T cells is largely dependent on the expression of class II MHC molecules by the APCs. Using immunohistochemistry, confocal microscopy, and flow cytometry, we demonstrate that α-smooth muscle actin+, CD90+ subepithelial myofibroblasts (stromal cells) constitutively express class II MHC molecules in normal colonic mucosa and that they are distinct from professional APCs such as macrophages and dendritic cells. Primary isolates of human colonic myofibroblasts (CMFs) cultured in vitro were able to stimulate allogeneic CD4+ T cell proliferation. This process was dependent on class II MHC and CD80/86 costimulatory molecule expression by the myofibroblasts. We also demonstrate that CMFs, engineered to express a specific DR4 allele, can process and present human serum albumin to a human serum albumin-specific and DR4 allele-restricted T cell hybridoma. These studies characterize a novel cell phenotype which, due to its strategic location and class II MHC expression, may be involved in capture of Ags that cross the epithelial barrier and present them to lamina propria CD4+ T cells. Thus, human CMFs may be important in regulating local immunity in the colon.

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“…18Co was used in the study as a representative IMF. Additional primary cultures of subepithelial small IMFs were generated according to the method described by Mahida et al (38,39) from full-thickness fresh human tissue samples. Full-thickness fresh human tissue samples were obtained from discarded surgical resection material of small intestine in compliance with protocols approved by the University of Texas Medical Branch Institutional Review Board.…”

Section: Primary Myofibroblast Culturesmentioning

confidence: 99%

Monocyte Chemoattractant Protein-1 Production by Intestinal Myofibroblasts in Response to Staphylococcal Enterotoxin A: Relevance to Staphylococcal Enterotoxigenic Disease

Pinchuk¹,

Beswick²,

Saada³

et al. 2007

The Journal of Immunology

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Food poisoning due to staphylococcal enterotoxins A and B (SEA and SEB) affects hundreds of thousands of people annually. SEA and SEB induce massive intestinal cytokine production, which is believed to be the key factor in staphylococcal enterotoxin enteropathy. MHC class II molecules are the major receptors for staphylococcal enterotoxins. We recently demonstrated that normal human subepithelial intestinal myofibroblasts (IMFs) express MHC class II molecules. We hypothesized that IMFs are among the first cells to respond to staphylococcal enterotoxins and contribute to the cytokine production associated with staphylococcal enterotoxin pathogenesis. We demonstrated here that primary cultured IMFs bind staphylococcal enterotoxins in a MHC class II-dependent fashion in vitro. We also demonstrated that staphylococcal enterotoxins can cross a CaCo-2 epithelial monolayer in coculture with IMFs and bind to the MHC class II on IMFs. IMFs responded to SEA, but not SEB, exposure with 3- to 20-fold increases in the production of proinflammatory chemokines (MCP-1, IL-8), cytokines (IL-6), and growth factors (GM-CSF and G-CSF). The SEA induction of the proinflammatory mediators by IMFs resulted from the efficient cross-linking of MHC class II molecules because cross-linking of class II MHC by biotinylated anti-HLA-DR Abs induced similar cytokine patterns. The studies presented here show that MCP-1 is central to the production of other cytokines elicited by SEA in IMFs because its neutralization with specific Abs prevented the expression of IL-6 and IL-8 by IMFs. Thus, MCP-1 may play a leading role in initiation of inflammatory injury associated with staphylococcal enterotoxigenic disease.

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Adult human colonic subepithelial myofibroblasts express extracellular matrix proteins and cyclooxygenase-1 and -2

Cited by 110 publications

References 25 publications

The increased mucosal mRNA expressions of complement C3 and interleukin-17 in inflammatory bowel disease

The increased mucosal mRNA expressions of complement C3 and interleukin-17 in inflammatory bowel disease

Subepithelial Myofibroblasts are Novel Nonprofessional APCs in the Human Colonic Mucosa

Monocyte Chemoattractant Protein-1 Production by Intestinal Myofibroblasts in Response to Staphylococcal Enterotoxin A: Relevance to Staphylococcal Enterotoxigenic Disease

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