Adult Stem Cell Therapies for Tissue Regeneration: Ex Vivo Expansion in an Automated System

Goltry, Kristin L.; Smith, Douglas M.; Dennis, James E.; Rowley, Jon A.; Bartel, Ronnda L.

doi:10.1007/978-1-4020-8502-4_14

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Effect Of Shear Stress On Stem Cells Grown In Bioreactors Fo1

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Cited by 2 publications

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“…Moreover, manipulations become more complicated and risk of contamination also increases. In spite of additional attempts to apply robotics and bioreactor control to static bioreactors [175,176], dynamic bioreactors ensuring 2-D or 3-D cell growth remain superior.…”

Section: Effect Of Shear Stress On Stem Cells Grown In Bioreactors Fomentioning

confidence: 99%

Mass Production of Mesenchymal Stem Cells — Impact of Bioreactor Design and Flow Conditions on Proliferation and Differentiation

Jossen¹,

Pörtner²,

Kaiser³

et al. 2014

Cells and Biomaterials in Regenerative Medicine

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Section: Effect Of Shear Stress On Stem Cells Grown In Bioreactors Fomentioning

confidence: 99%

Mass Production of Mesenchymal Stem Cells — Impact of Bioreactor Design and Flow Conditions on Proliferation and Differentiation

Jossen¹,

Pörtner²,

Kaiser³

et al. 2014

Cells and Biomaterials in Regenerative Medicine

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Proteomic Characteristics of ex vivo-Enriched Adult Human Bone Marrow Mononuclear Cells in Continuous Perfusion Cultures

Prahalad

Hock

2009

J. Proteome Res.

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A major challenge in developing cell therapies is reliable characterization of the cell product at the molecular level. Fresh autologous and passaged human bone marrow enriched for stem and mesenchymal stromal stem cells have been used to regenerate bone. We report the proteome of an innovative autologous human bone marrow-derived mixed cell product (BMMCP), cultured ex vivo for 12 days, in automated continuous media perfusion system to avoid passaging, and discuss reproducibility of protein composition. Each BMMCP is compared to its originating human adult bone marrow mononuclear cells (BMMNC). With the use of 2-D LC-MS/MS approach, 638 (BMMNC) and 867 (BMMCP) distinct proteins were identified including cell adhesion molecules, extracellular matrix and growth factors. Overlap of protein identifications revealed that 67% of the BMMNC proteome was retained in the BMMCP, and protein expression of selected cell lineages was enhanced. Isotope-coded affinity tags (ICAT) and MS/MS were used to identify and quantify relative changes in the proteome of BMMNC and their related BMMCP, obtained from 3 separate donors. In 3 separate ICAT experiments, 57% of proteome identified was shared between donors. Measurable and definable proteomic characterization of BMMCP will facilitate their use in clinical trials and provide insight into cell functionality needed to support multiple therapeutic indications.

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Adult Stem Cell Therapies for Tissue Regeneration: Ex Vivo Expansion in an Automated System

Cited by 2 publications

References 61 publications

Mass Production of Mesenchymal Stem Cells — Impact of Bioreactor Design and Flow Conditions on Proliferation and Differentiation

Mass Production of Mesenchymal Stem Cells — Impact of Bioreactor Design and Flow Conditions on Proliferation and Differentiation

Proteomic Characteristics of ex vivo-Enriched Adult Human Bone Marrow Mononuclear Cells in Continuous Perfusion Cultures

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