Adulteration in medicinally important plant species of Ziziphora in Iran market: DNA barcoding approach

Sheidai, Masoud; Tabaripour, Raheleh; Talebi, Seyed Mehdi; Noormohammadi, Zahra; Koohdar, Fahimeh

doi:10.1016/j.indcrop.2019.01.025

Cited by 27 publications

(19 citation statements)

References 25 publications

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“…olive, and are given for blood pressure control. Therefore, we decided to investigate this matter by using the modern molecular approach of DNA barcoding (Sheidai et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…A combination of various approaches and disciplines have been considered for such investigations such as physical and chemical methods like thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC), external morphological features, DNA sequence similarity, and phylogenetic analyses using both tree-based criteria or character-based methods (Pasqualone et al 2016;Ghorbani et al 2017;Yu et al 2018). Recently, determination of the genetic distance among the studied samples and with the correct plant specimens as well as BLAST approach and DNA barcoding have also been used (Sheidai et al 2019). A DNA barcode is unique short DNA sequences that different plant species, cultivars or races from each other and the closely related plant taxa (Sheidai et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, determination of the genetic distance among the studied samples and with the correct plant specimens as well as BLAST approach and DNA barcoding have also been used (Sheidai et al 2019). A DNA barcode is unique short DNA sequences that different plant species, cultivars or races from each other and the closely related plant taxa (Sheidai et al 2019). It enables the researchers to correctly identify the target species.…”

Section: Introductionmentioning

confidence: 99%

“…It enables the researchers to correctly identify the target species. This approach has been utilized to identify the medicinal plants and distinguish the original products from adulterated species/DNA (Heubl 2010;Sheidai et al 2019). The present study was conducted with the following aims: 1-To use different DNA regions to differentiate wild versus cultivated olive plants, 2-To provide proper barcodes for either species, 3-To check the potential adulteration/ or misidentification of these plant products by the local medicinal plant markets based on different barcoding approaches.…”

Section: Introductionmentioning

confidence: 99%

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The use of DNA barcoding to avoid adulteration in olive plant leaf products

et al. 2021

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Abstract. Partovi R, Iranbakhsh A, Sheidai M, Ebadi M. 2020. The use of DNA barcoding to avoid adulteration in olive plant leaf products. Asian J For 5: 42-47. The leaves of olive plant species Olea europeae, and O. europeae var. cuspidata have been used for medicinal perfused in Iran. The first species leaves have been used to control the blood pressure, while the leaves of wild olive have been used for abortion by locals. Our preliminary inspection of the medicinal plant market revealed that the leaves of these two olive species are sold mistakenly to the consumers and their health might be at risk. Therefore, we permed this investigation to produce DNA barcodes for correct identification of these two olive species and also identify the potential adulteration in our local market. We used Internal transcribed spacer (ITS), as well as plastid genome trnL-F intergenic spacer and ribosomal protein L16 (rpL16) sequences. These sequences after alignment and curation produced DNA barcodes that can differentiate the two olive species from each other. The phylogenetic trees constructed also separated the samples of these olive species and confirmed the potential use of these short DNA sequences for olive barcoding. The present study revealed that some of the local shops mistakenly sell the wild olive leaves instead of the cultivated olive leaf to be used for blood pressure. This mistake endangers the health of pregnant women consumers if they carry a child. We suggest using a combination of nuclear ITS and plastid intergenic spacer (trnL-F and rpL16) regions for DNA barcoding of olive plants to avoid leaf product adulteration.

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“…olive, and are given for blood pressure control. Therefore, we decided to investigate this matter by using the modern molecular approach of DNA barcoding (Sheidai et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The use of DNA barcoding to avoid adulteration in olive plant leaf products

et al. 2021

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“…Yao et al evaluated the ITS2 barcodes from 50,790 plants and 12,221 animals [24]. Scientists have used rDNA-internal transcribed spacers (ITS), rbcL , matK , psbA - trnH, and trnL-F to identify plant species and analyse phylogenetic relationships [25,26,27,28]. Because of the difference in amplification efficiency among species and the different needs of primer generality, barcodes were screened by combining genes.…”

Section: Introductionmentioning

confidence: 99%

Combining DNA Barcoding and HPLC Fingerprints to Trace Species of an Important Traditional Chinese Medicine Fritillariae Bulbus

et al. 2019

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Fritillariae Bulbus is a precious Chinese herbal medicine that is grown at high elevation and used to relieve coughs, remove phlegm, and nourish the lungs. Historically, Fritillariae Bulbus has been divided into two odourless crude drugs: Fritillariae Cirrhosae Bulbus and Fritillariae Thunbergii Bulbus. However, now the Chinese Pharmacopoeia has described five Fritillariae Bulbus—the new additions include Fritillariae Pallidiflorae Bulbus, Fritillariae Ussuriensis Bulbus, and Fritillariae Hupehensis Bulbus. Because the morphology of dried Fritillariae Bulbus is similar, it is difficult to accurately identify the different types of Fritillariae Bulbus. In the current study, we develop a method combining DNA barcoding and high-performance liquid chromatography (HPLC) to help distinguish Fritillariae Cirrhosae Bulbus from other Fritillariae Bulbus and guarantee species traceability of the five types of Fritillariae Bulbus. We report on the validation of an integrated analysis method for plant species identification using DNA barcoding that is based on genetic distance, identification efficiency, inter- and intra-specific variation, calculated nearest distance, neighbour-joining tree and barcoding gap. Our results show that the DNA barcoding data successfully identified the five Fritillariae Bulbus by internal transcribed spacer region (ITS) and ITS2, with the ability to distinguish the species origin of these Fritillariae Bulbus. ITS2 can serve as a potentially useful DNA barcode for the Fritillaria species. Additionally, the effective chemical constituents are identified by HPLC combined with a chemical identification method to classify Fritillaria. The HPLC fingerprint data and HCA (hierarchical clustering analysis) show that Fritillariae Cirrhosae Bulbus is clearly different from Fritillariae Thunbergii Bulbus and Fritillariae Hupehensis Bulbus, but there is no difference between Fritillariae Cirrhosae Bulbus, Fritillariae Ussuriensis Bulbus, and Fritillariae Pallidiflorae Bulbus. These results show that DNA barcoding and HPLC fingerprinting can discriminate between the five Fritillariae Bulbus types and trace species to identify related species that are genetically similar.

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Combination of c oxidase subunit I based deoxyribonucleic acid barcoding and HPLC techniques for the identification and quality evaluation of Pheretima aspergillum

Liu

Zhang

et al. 2020

J of Separation Science

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This study aimed to identify Pheretima aspergillum (Guang‐Pheretima) and its adulterants using the cytochrome c oxidase subunit I based deoxyribonucleic acid barcoding technology, and further to evaluate their quality using an optimized high‐performance liquid chromatography method. For deoxyribonucleic acid barcoding identification, the Kimura‐2‐Parameter model was used to analyze genetic distance, and phylogenetic neighbor‐joining tree was constructed for species identification of 20 labeled Guang‐Pheretima samples. A high‐performance liquid chromatography method was developed for the simultaneous determination of seven nucleoside components for quality evaluation. Compared with the GenBank database, 10 samples were identified as real Guang‐Pheretima (P. aspergillum), and the others as the adulterants‐Metaphire magna. The maximum intraspecific genetic distances of c oxidase subunit I sequence for P. aspergillum were smaller than the minimum interspecific genetic distances between P. aspergillum and M. magna. Ten P. aspergillum and 10 M. magna samples were clearly clustered in the neighbor‐joining tree. The contents of seven nucleosides components in P. aspergillum were significantly higher than that in its adulterant‐M. magna. The incidence of adulterants for Guang‐Pheretima was high (up to 50%) with an alarming quality. This study provided a powerful idea for the quality evaluation of other highly valuable plant‐ or animal‐derived products for safety concerns to avoid misidentification.

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Adulteration in medicinally important plant species of Ziziphora in Iran market: DNA barcoding approach

Cited by 27 publications

References 25 publications

The use of DNA barcoding to avoid adulteration in olive plant leaf products

The use of DNA barcoding to avoid adulteration in olive plant leaf products

Combining DNA Barcoding and HPLC Fingerprints to Trace Species of an Important Traditional Chinese Medicine Fritillariae Bulbus

Combination of c oxidase subunit I based deoxyribonucleic acid barcoding and HPLC techniques for the identification and quality evaluation of Pheretima aspergillum

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