Advance in flavonoids biosynthetic pathway and synthetic biology

Zou, Li-Qiu; Wang, Caixia; Kuang, Xue-Jun; Liu, Ying; Sun, Chao

doi:10.4268/cjcmm20162207

Cited by 9 publications

(5 citation statements)

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“…In this study, we observed distinct gene expression profiles among three ESMs from Hubei, Jiangxi, and Anhui, with particular attention to the AHHS from Anhui, which exhibited the lowest flavonoid levels ( Figure 2C ). Despite identifying differential gene expression in AHHS compared to the other two ESMs, the majority of flavonoid biosynthesis-related genes detected did not elucidate the precise causes of the varied metabolite accumulation ( Zou et al., 2016 ; Liu et al., 2021 ; Lou et al., 2021 ). Two primary reasons are posited: (1) the complete biosynthetic pathways of major flavonoids such as icariin and epimedin remain elusive to date ( Huang et al., 2015 ; Wang et al., 2021 ), precluding the direct detection of gene expression responsible for their biosynthesis; (2) the absence of a reference genome for ESMs hinders the examination of genomic variations among these samples.…”

Section: Discussionmentioning

confidence: 93%

Metabolomic and transcriptomic analysis of the flavonoid biosynthesis pathway in Epimedium sagittatum (Sieb. et Zucc.) Maxim. from distinct locations

Tian,

Lv,

et al. 2024

Front. Plant Sci.

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Epimedium sagittatum (Sieb. et Zucc.) Maxim. (ESM) which accumulates several principal flavonoid compounds including epimedin A, B, C and icariin, is extensively utilized in traditional herbs for sexual dysfunction, osteoporosis etc. In China, ESM has a wealth of wild plant resources and characterized by significant variability in medicinal compounds accumulation. Understanding the diversity of ESMs can lead to better utilization of these plant resources. In this study, we integrated the metabolomic and transcriptomic analysis of three ESMs that originated in Anhui, Hubei and Jiangxi in China. Results showed that the flavonoid biosynthesis as well as the related gene expression in these ESMs revealed substantial differences. For example, the epimedin A, B, C and icariin as well as some related gene expression in ESMs from Anhui are significantly lower than those of in others. These results suggested that the ESMs from wild population without quality checkout may not be suitable for directly use as the materials for preparation of Chinese medicine and ESMs with different accumulation of metabolites could be used for distinct applications.

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Section: Discussionmentioning

confidence: 93%

Metabolomic and transcriptomic analysis of the flavonoid biosynthesis pathway in Epimedium sagittatum (Sieb. et Zucc.) Maxim. from distinct locations

Tian,

Lv,

et al. 2024

Front. Plant Sci.

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“…It serves as the main route for flavonoid biosynthesis, with the majority of flavonoids and dihydroflavonoids being synthesized through this pathway. On the other hand, the phenylalanine metabolic pathway offers an alternate route for the biosynthesis of specific isoflavonoids and flavonol compounds [20,21]. The biosynthesis of flavonoid compounds is tightly regulated by various biosynthetic enzymes and genes, including PAL, 4CL, CHS, FLS, and F3 ′ H [22,23].…”

Section: Discussionmentioning

confidence: 99%

The Effect and Potential Mechanism of Fulvic Acid on Flavonoids in Lemon Leaves

Ren,

Yang,

Dai

et al. 2024

Horticulturae

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Citrus limon (L.) Burm. f. is a horticultural crop known for its abundance of valuable secondary metabolites, including flavonoids, which are found in its fruits and leaves. Our previous research has shown that treating C. limon with fulvic acid (FA) can enhance the levels of vitamin C, total acid, total sugar, total flavonoids, and phenols in its fruits as well as the volatiles and total flavonoids in its leaves. In this current study, we established a method to analyze eight specific flavonoids in lemon leaves and evaluated the impact of irrigation with FA on the content of these flavonoids over a six-month period using HPLC-DAD/MS analysis. Moreover, we investigated the potential mechanisms of FA through ELISA and q-PCR methods. The results indicated that FA increased the contents of four flavonoids, namely, isoorientin, eriocitrin, vitexin, and rutin, and promoted the activity and gene expression of phenylalanineammonialyase (PAL), 4-coumaric acid coenzyme A ligase (4CL), chalcone synthase (CHS), flavonoid 3′-hydroxylase (F3′H), and flavonol synthase (FLS). Furthermore, the relationship between flavonoid content and the activities of biosynthetic enzymes was analyzed using orthogonal partial least squares discriminant analysis (OPLS-DA), which revealed a positive correlation between seven flavonoid levels and the activity of five biosynthetic enzymes under FA treatment.

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“…In the flavonoid biosynthesis pathway of sample S8, C4H catalyzed the production of coumaroyl-CoA, CHS facilitated chalcone formation, and naringenin is produced by isomerization of CHI. Dihydroflavonoids are crucial precursors of flavonoids [38][39][40]. Up-regulation of the aforementioned genes in the flavonoid biosynthesis pathway of sample S8 promoted the accumulation of dihydro flavonoids and facilitated the production of isoflavones, flavones, and dihydro flavanols under the catalysis of various enzymes, consequently increasing flavonoid content.…”

Section: Discussionmentioning

confidence: 99%

DNA barcoding identification of grafted Semen Ziziphi Spinosae and transcriptome study of wild Semen Ziziphi Spinosae

Wu,

Guo,

Zhao

et al. 2023

PLoS ONE

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Semen Ziziphi Spinosae (SZS) is the dried and ripe seeds of Ziziphus jujuba var. spinosa. Currently, the yield of naturally grown SZS is unstable owing to environmental factors. Grafting high-quality sour jujube scions onto sour jujube or jujube tree stocks can result in a greater yield. However, the effects of grafting on the quality and gene expression of SZS have rarely been reported. This study used a DNA barcoding technique, high-performance liquid phase-evaporative luminescence detector (HPLC-ELSD), and transcriptomics to investigate the origin and genetic differences between grafted and wild jujube seeds. DNA barcoding identified all samples as Ziziphus jujuba var. spinosa. HPLC-ELSD analysis revealed a higher content of grafted SZS compared to that of the wild SZS. Transcriptome analysis of the metabolic pathways in SZS showed that 22 and 19 differentially expressed gene sequences encoded enzymes related to flavonoids and saponin synthesis, respectively. Weighted correlation network analysis (WGCNA) identified 15 core genes governing the differences in medicinal components between grafted and wild SZS. This study demonstrated the use of DNA barcoding and fingerprint methods to identify jujube seed species and effectively capture ingredient information of medicinal materials. Additionally, transcriptome technology provided data for identifying core differential genes, facilitating studies on quality differences between grafted and wild SZS.

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Advance in flavonoids biosynthetic pathway and synthetic biology

Cited by 9 publications

References 0 publications

Metabolomic and transcriptomic analysis of the flavonoid biosynthesis pathway in Epimedium sagittatum (Sieb. et Zucc.) Maxim. from distinct locations

Metabolomic and transcriptomic analysis of the flavonoid biosynthesis pathway in Epimedium sagittatum (Sieb. et Zucc.) Maxim. from distinct locations

The Effect and Potential Mechanism of Fulvic Acid on Flavonoids in Lemon Leaves

DNA barcoding identification of grafted Semen Ziziphi Spinosae and transcriptome study of wild Semen Ziziphi Spinosae

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