Additional information is available at the end of the chapter http://dx.doi.org/10.5772/52141
IntroductionVirgin olive oil (VOO) is obtained exclusively by mechanical extraction from the olive fruit and can be consumed crude without any further physical-chemical treatments of refining. Its sensory and health properties are intimately linked to its chemical characteristics, in particular to several minor components, which are strongly affected by the operative conditions of oil processing and can thus be considered as analytical markers of the quality of oil processing.VOO contains different classes of phenolic compounds, such as phenolic acids, phenolic alcohols, hydroxy-isochromans, flavonoids, secoiridoids and lignans. The phenolic acids together with phenyl-alcohols, hydroxy-isochromans and flavonoids are present in small amounts in VOO (Montedoro et al., 1992). Secoiridoids which, in combination with lignans, are the main hydrophilic phenols of VOO, include the dialdehydic form of decarboxymethyl elenolic acid linked to 3,4-DHPEA or p-HPEA (3,4-DHPEA-EDA or p-HPEA-EDA) an isomer of the oleuropein aglycon (3,4-DHPEA-EA) and the ligstroside aglycon (p-HPEA-EA). The main lignans found in VOO are (+)-1-acetoxypinoresinol and (+)-1-pinoresinol (Brenes et al., 2000;Owen et al., 2000). Both secoiridoids and lignans affect the quality of the sensory and health properties of VOO (Servili et al., 2004a), determining bitter, pungent sensations.Many compounds, mainly carbonyl compounds, alcohols, esters and hydrocarbons, have been found in the volatile fraction of VOO.The C6 and C5 compounds, especially C6 linear unsaturated and saturated aldehydes alcohols and esters represent the most important fraction of volatile compounds found in high quality VOOs.The C6 and C5 compounds, produced from polyunsaturated fatty acids by the enzymatic activities exerted by the lipoxygenase (LOX) pathway and their concentrations, depend on the level and the activity of each enzyme involved in this LOX pathway.Olive Germplasm -The Olive Cultivation, The pathway (Figure 1) begins with the production of 9-and 13-hydroperoxides of linoleic (LA) and linolenic (LnA) acids mediated by lipoxygenase (LOX). The subsequent cleavage of 13-hydroperoxides is catalysed by very specific hydroperoxide lyases (HPL) and leads to C6 aldehydes, of which the unsaturated aldehydes can isomerize from cis-3 to the more stable trans-2 form. The mediation of alcohol dehydrogenase (ADH) reduces C6 aldehydes to corresponding alcohols, which can produce esters due to the catalytic activity of alcohol acetyl transferases (AAT).Furthermore, an additional branch of the LOX pathway (Figure 1) is active when the substrate is LnA. LOX would catalyse the formation of stabilized 1,3-pentene radicals, which can either dimerize leading to C10 hydrocarbons (known as pentene dimers), or can react with a hydroxy radical, producing C5 alcohols. The latter can be enzymatically oxidated to corresponding C5 carbonyl compounds. These compounds are responsible for the most important s...