Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications

Maharjan, Sushila; Ma, Chenshuo; Singh, Bibhor; Kang, Heemin; Orive, Gorka; Yao, Junjie; Shrike Zhang, Yu

doi:10.1016/j.addr.2024.115237

Cited by 17 publications

(1 citation statement)

References 312 publications

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“…Advances in the development of spheroids and organoids and their application in various fields of research, including clarifying cellular and molecular aspects, have been enabled by the rise of new technological approaches frameable within high-throughput experimental workflows, such as 3D bioprinting or microfluidic techniques [ 31 , 32 ]. 3D bioprinting uses bio-ink, comprising living organoids or spheroids encapsulated within tunable-biomaterial, to precisely create 3D biological geometries mimicking those of the native tissue in a layer-by-layer approach [ 33 ]. This technology has rapidly emerged as a promising tool for the creation of 3D cell models with a well-defined architecture, composition and high reproducibility, particularly useful for tumor–stroma investigation and drug screening applications.…”

Section: Introductionmentioning

confidence: 99%

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Bloise,

Giannaccari,

Guagliano

et al. 2024

Cells

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Over the past decade, the development of three-dimensional (3D) models has increased exponentially, facilitating the unravelling of fundamental and essential cellular mechanisms by which cells communicate with each other, assemble into tissues and organs and respond to biochemical and biophysical stimuli under both physiological and pathological conditions. This section presents a concise overview of the most recent updates on the significant contribution of different types of 3D cell cultures including spheroids, organoids and organ-on-chip and bio-printed tissues in advancing our understanding of cellular and molecular mechanisms. The case studies presented include the 3D cultures of breast cancer (BC), endometriosis, the liver microenvironment and infections. In BC, the establishment of 3D culture models has permitted the visualization of the role of cancer-associated fibroblasts in the delivery of exosomes, as well as the significance of the physical properties of the extracellular matrix in promoting cell proliferation and invasion. This approach has also become a valuable tool in gaining insight into general and specific mechanisms of drug resistance. Given the considerable heterogeneity of endometriosis, 3D models offer a more accurate representation of the in vivo microenvironment, thereby facilitating the identification and translation of novel targeted therapeutic strategies. The advantages provided by 3D models of the hepatic environment, in conjunction with the high throughput characterizing various platforms, have enabled the elucidation of complex molecular mechanisms underlying various threatening hepatic diseases. A limited number of 3D models for gut and skin infections have been developed. However, a more profound comprehension of the spatial and temporal interactions between microbes, the host and their environment may facilitate the advancement of in vitro, ex vivo and in vivo disease models. Additionally, it may pave the way for the development of novel therapeutic approaches in diverse research fields. The interested reader will also find concluding remarks on the challenges and prospects of using 3D cell cultures for discovering cellular and molecular mechanisms in the research areas covered in this review.

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Section: Introductionmentioning

confidence: 99%

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Bloise,

Giannaccari,

Guagliano

et al. 2024

Cells

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Organoids of Musculoskeletal System for Disease Modeling, Drug Screening, and Regeneration

Chen,

Liu,

et al. 2024

Adv Healthcare Materials

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Musculoskeletal diseases have emerged as the leading cause of disability worldwide, with their prevalence increasing annually. In light of this escalating health challenge, organoids, an emerging technology in tissue engineering, offer promising solutions for disease modeling, drug screening, regeneration, and repair processes. The successful development of musculoskeletal organoids represents a significant breakthrough, providing a novel platform for studying musculoskeletal diseases and facilitating the discovery of new treatments. Moreover, organoids serve as valuable complements to traditional 2D culture methods and animal models, offering rich insights into musculoskeletal biology. This review provides an overview of organoid technology, outlining the construction processes of various musculoskeletal organoids and highlighting their similarities and differences. Furthermore, the challenges associated with organoid technology in musculoskeletal systems are discussed and insights into future perspectives are offered.

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4D bioprinting: a review on smart bio-adaptable technology to print stimuli-responsive materials

Gara,

Gujjala,

Prasad

et al. 2024

Prog Addit Manuf

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Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications

Cited by 17 publications

References 312 publications

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Organoids of Musculoskeletal System for Disease Modeling, Drug Screening, and Regeneration

4D bioprinting: a review on smart bio-adaptable technology to print stimuli-responsive materials

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