Advanced gellan gum-based glycol chitosan hydrogel for cartilage tissue engineering biomaterial

Lee, Sumi; Choi, Joo Hee; Park, Ain; Rim, Mina; Youn, Jina; Lee, Wonchan; Song, Jeong Eun; Khang, Gilson

doi:10.1016/j.ijbiomac.2020.04.135

Cited by 46 publications

(13 citation statements)

References 37 publications

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“…On 21 days of culture, all the groups showed an increased dsDNA content and, especially, the 0.5% GG/3.5% SF/CS group showed the highest dsDNA content. It is regarded that, in addition to the cell attachment site, physical support is significant for cells to proliferate in the hydrogel microenvironment [61]. μg/mL and 0.4 ± 0.07 μg/mL in 0.3% GG/3.7% SF/CS and 0.5% GG/3.5% SF/CS, respectively.…”

Section: Dsdna and Gagmentioning

confidence: 99%

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

et al. 2021

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Hydrogel is in the spotlight as a useful biomaterial in the field of drug delivery and tissue engineering due to its similar biological properties to a native extracellular matrix (ECM). Herein, we proposed a ternary hydrogel of gellan gum (GG), silk fibroin (SF), and chondroitin sulfate (CS) as a biomaterial for cartilage tissue engineering. The hydrogels were fabricated with a facile combination of the physical and chemical crosslinking method. The purpose of this study was to find the proper content of SF and GG for the ternary matrix and confirm the applicability of the hydrogel in vitro and in vivo. The chemical and mechanical properties were measured to confirm the suitability of the hydrogel for cartilage tissue engineering. The biocompatibility of the hydrogels was investigated by analyzing the cell morphology, adhesion, proliferation, migration, and growth of articular chondrocytes-laden hydrogels. The results showed that the higher proportion of GG enhanced the mechanical properties of the hydrogel but the groups with over 0.75% of GG exhibited gelling temperatures over 40 °C, which was a harsh condition for cell encapsulation. The 0.3% GG/3.7% SF/CS and 0.5% GG/3.5% SF/CS hydrogels were chosen for the in vitro study. The cells that were encapsulated in the hydrogels did not show any abnormalities and exhibited low cytotoxicity. The biochemical properties and gene expression of the encapsulated cells exhibited positive cell growth and expression of cartilage-specific ECM and genes in the 0.5% GG/3.5% SF/CS hydrogel. Overall, the study of the GG/SF/CS ternary hydrogel with an appropriate content showed that the combination of GG, SF, and CS can synergistically promote articular cartilage defect repair and has considerable potential for application as a biomaterial in cartilage tissue engineering.

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Section: Dsdna and Gagmentioning

confidence: 99%

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

et al. 2021

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“…The selection of cell type is an important factor to be considered when testing different bone grafts for cytotoxicity, biocompatibility, and osteoinduction properties. An established osteogenic cell isolated from the periosteum is an excellent cell type for this purpose [ 28 ]. Recently, mesenchymal stromal cells derived from bone marrow have been identified as optimal since they are the precursors of osteoblasts and possess an excellent proliferative capacity.…”

Section: Discussionmentioning

confidence: 99%

Calcium-Silicate-Incorporated Gellan-Chitosan Induced Osteogenic Differentiation in Mesenchymal Stromal Cells

et al. 2021

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Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) w/w was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS–seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (w/w) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects.

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“…A wide range of natural and synthetic biopolymers [ 18 , 43 ] have been tested to produce hydrogels such as chitosan [ 44 , 45 ], collagen [ 46 ] or gelatin [ 47 , 48 ], alginate [ 49 ], hyaluronic acid [ 50 , 51 ], heparin [ 52 , 53 ], chondroitin sulfate [ 54 ], poly(ethylene glycol) (PEG) [ 55 ], and poly(vinyl alcohol) [ 56 ] ( Figure 3 ). Table 2 summarizes the studies on the most popular biopolymers used to repair cartilage.…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

“…The viscoelasticity of hydrogels permits load transmission to chondrocytes [ 43 ]. Although different hydrogels produced by various procedures have been investigated in re-cent years, rarely have any integrated hydrogels been used in the regenerative medical profession.…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

Advanced Hydrogels for Cartilage Tissue Engineering: Recent Progress and Future Directions

et al. 2021

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Cartilage is a tension- and load-bearing tissue and has a limited capacity for intrinsic self-healing. While microfracture and arthroplasty are the conventional methods for cartilage repair, these methods are unable to completely heal the damaged tissue. The need to overcome the restrictions of these therapies for cartilage regeneration has expanded the field of cartilage tissue engineering (CTE), in which novel engineering and biological approaches are introduced to accelerate the development of new biomimetic cartilage to replace the injured tissue. Until now, a wide range of hydrogels and cell sources have been employed for CTE to either recapitulate microenvironmental cues during a new tissue growth or to compel the recovery of cartilaginous structures via manipulating biochemical and biomechanical properties of the original tissue. Towards modifying current cartilage treatments, advanced hydrogels have been designed and synthesized in recent years to improve network crosslinking and self-recovery of implanted scaffolds after damage in vivo. This review focused on the recent advances in CTE, especially self-healing hydrogels. The article firstly presents the cartilage tissue, its defects, and treatments. Subsequently, introduces CTE and summarizes the polymeric hydrogels and their advances. Furthermore, characterizations, the advantages, and disadvantages of advanced hydrogels such as multi-materials, IPNs, nanomaterials, and supramolecular are discussed. Afterward, the self-healing hydrogels in CTE, mechanisms, and the physical and chemical methods for the synthesis of such hydrogels for improving the reformation of CTE are introduced. The article then briefly describes the fabrication methods in CTE. Finally, this review presents a conclusion of prevalent challenges and future outlooks for self-healing hydrogels in CTE applications.

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Advanced gellan gum-based glycol chitosan hydrogel for cartilage tissue engineering biomaterial

Cited by 46 publications

References 37 publications

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

Calcium-Silicate-Incorporated Gellan-Chitosan Induced Osteogenic Differentiation in Mesenchymal Stromal Cells

Advanced Hydrogels for Cartilage Tissue Engineering: Recent Progress and Future Directions

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