Advanced glycated albumin impairs HDL anti-inflammatory activity and primes macrophages for inflammatory response that reduces reverse cholesterol transport

Okuda, Ligia Shimabukuro; Castilho, Gabriela; Rocco, Débora Dias Ferraretto Moura; Nakandakare, Edna Regina; Catanozi, Sérgio; Passarelli, Marisa

doi:10.1016/j.bbalip.2012.08.011

Cited by 45 publications

(49 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…AGE-albumin is serum albumin modified by AGEs [4], and its interaction with RAGE primes macrophage for inflammatory response and aggravates inflammation-related diseases [4].…”

Section: Introductionmentioning

confidence: 99%

Age-related accumulation of advanced glycation end-products-albumin, S100β, and the expressions of advanced glycation end product receptor differ in visceral and subcutaneous fat

Son

Ahn

et al. 2016

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

“…AGE-albumin is serum albumin modified by AGEs [4], and its interaction with RAGE primes macrophage for inflammatory response and aggravates inflammation-related diseases [4].…”

Section: Introductionmentioning

confidence: 99%

Age-related accumulation of advanced glycation end-products-albumin, S100β, and the expressions of advanced glycation end product receptor differ in visceral and subcutaneous fat

Son

Ahn

et al. 2016

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

“…Body weight (BW; g) was monitored weekly and percentual body weight gain was calculated using the following equation: (initial BW -final BW)/initial BW. Advanced glycated albumin preparation Rat serum albumin modified by advanced glycation (AlbAGE) was prepared as previously described [22]. Briefly, fatty acid and endotoxin free rat serum albumin (Sigma-Aldrich, Taufkirche, Germany) was incubated in vitro, for 4 days, under nitrogen atmosphere, at 37 °C, in the dark, under sterile conditions in a water bath shaker, with 10 mM glycoaldehyde (Sigma) dissolved in phosphate buffered saline (PBS) and EDTA (pH 7.4).…”

Section: Ethics Statementmentioning

confidence: 99%

N-Acetyl Cysteine Attenuated the Deleterious Effects of Advanced Glycation End-Products on the Kidney of Non-Diabetic Rats

Thieme

Silva

Fabre

et al. 2016

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Aim: To assess the renal effects of chronic exposure to advanced glycation end-products (AGEs) in the absence of diabetes and the potential impact of concomitant treatment with the antioxidant N-acetyl cysteine (NAC). Methods: Wistar rats received intraperitoneally 20 mg/kg/day of albumin modified (AlbAGE) or not (AlbC) by advanced glycation for 12 weeks and oral NAC (600mg/L; AlbAGE+NAC and AlbC+NAC, respectively). Biochemical, urinary and renal morphological analyses; carboxymethyl-lysine (CML, an AGE), CD68 (macrophage infiltration), and 4-hydroxynonenal (4-HNE, marker of oxidative stress) immunostaining; intrarenal mRNA expression of genes belonging to pathways related to AGEs (Ager, Ddost, Nfkb1), renin-angiotensin system (Agt, Ren, Ace), fibrosis (Tgfb1, Col4a1), oxidative stress (Nox4, Txnip), and apoptosis (Bax, Bcl2); and reactive oxidative species (ROS) content were performed. Results: AlbAGE significantly increased urine protein-to-creatinine ratio; glomerular area; renal CML content and macrophage infiltration; expression of Ager, Nfkb1, Agt, Ren, Tgfb1, Col4a1, Txnip, Bax/Bcl2 ratio; and 4-HNE and ROS contents. Some of these effects were attenuated by NAC concomitant treatment. Conclusion: Because AGEs are highly consumed in modern diets and implicated in the progression of different kidney diseases, NAC could be a therapeutic intervention to decrease renal damage, considering that long-term restriction of dietary AGEs is difficult to achieve in practice.

show abstract

“…Considerando que as alterações inflamatórias em macrófagos não necessariamente refletem a resposta inflamatória na parede arterial, já que outros componentes celulares, tais como linfócitos, neutrófilos, células musculares lisas e endoteliais, também estão envolvidos nesse processo, foram determinados a expressão e o conteúdo proteico de citocinas na aorta de camundongos, conforme protocolo validado em outros estudos (Dinarello, 2009;Hansson e Hermansson, 2011 (Okuda et al, 2012;Zhao et al, 2013). Adicionalmente, observou-se que a atividade dos receptores ABCA-1 e ABCG-1 foi prejudicada, pois o grupo PALM INTER apresentou redução no efluxo de colesterol para apo-AI e HDL 2 (Figura 15).…”

Section: Gorduras Interesterificadas E Efluxo Celular De Colesterolunclassified

Efeito das gorduras interesterificadas sobre o desenvolvimento da lesão aterosclerótica em camundongos knockout para o receptor de LDL

Afonso¹

View full text Add to dashboard Cite

Advanced glycated albumin impairs HDL anti-inflammatory activity and primes macrophages for inflammatory response that reduces reverse cholesterol transport

Cited by 45 publications

References 40 publications

Age-related accumulation of advanced glycation end-products-albumin, S100β, and the expressions of advanced glycation end product receptor differ in visceral and subcutaneous fat

Age-related accumulation of advanced glycation end-products-albumin, S100β, and the expressions of advanced glycation end product receptor differ in visceral and subcutaneous fat

N-Acetyl Cysteine Attenuated the Deleterious Effects of Advanced Glycation End-Products on the Kidney of Non-Diabetic Rats

Efeito das gorduras interesterificadas sobre o desenvolvimento da lesão aterosclerótica em camundongos knockout para o receptor de LDL

Contact Info

Product

Resources

About