Advanced Glycation Endproducts Accelerate Calcification in Microvascular Pericytes

Yamagishi, Sho‐ichi; Fujimori, Hideki; Yonekura, Hideto; Tanaka, Nobushige; Yamamoto, Hiroshi

doi:10.1006/bbrc.1999.0625

Cited by 106 publications

(78 citation statements)

References 15 publications

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“…[20] Also, advanced glycation end products accelerated calcification in microvascular pericytes. [21] Our study demonstrates that AOPP markedly increases the intracellular oxidative stress of HASMCs at 2 h, and that this effect peaks at 6 h. Vitamin E not only attenuated the intracellular oxidative stress induced by AOPP, but also blocked AOPP-induced OPN expression and calcium deposition. These results are consistent with an important role of AOPP in accelerating osteoblast differentiation and calcification of HASMCs through enhancing oxidative stress.…”

Section: Discussionsupporting

confidence: 53%

“…Oxidative stress has also been shown to cause the smooth muscle cell to dedifferentiate. [19][20][21][22] The present study investigates the role of AOPPs, prepared with human serum albumin (HSA) and hypochloric acid (HOCl), in modulating osteoblast differentiation and calcification of human aortic smooth muscle cells (HASMCs) in vitro. Then, we investigate the role of oxidative stress and the possible signal transduction pathway of AOPPs on HASMCs differentiation and calcification.…”

Section: Introductionmentioning

confidence: 99%

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Advanced Oxidation Protein Products Induce Vascular Calcification by Promoting Osteoblastic Trans-Differentiation of Smooth Muscle Cells via Oxidative Stress and ERK Pathway

et al. 2009

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Vascular calcification is an actively regulated process similar to bone formation. Advanced oxidation protein products (AOPPs) have been demonstrated to be novel markers of oxidantmediated protein damage. The present study investigated the role of AOPPs in inducing osteoblastic trans-differentiation and calcification of smooth muscle cells in vitro. We found that AOPPs directly increased the calcium deposition and expression of core binding factor-a1 (CBF-a1) and osteopontin (OPN) and significantly decreased SM-a-actin expression in human aortic smooth muscle cells (HASMCs). AOPPs increased intracellular oxidative stress, which was inhibited by vitamin E. Vitamin E also inhibited AOPP-induced calcium content and osteoblast differentiation of HASMCs. Furthermore, the inhibitor of ERK significantly suppressed the effects of AOPPs on calcification and osteoblast marker expression. These findings suggest that AOPPs induce vascular calcification by promoting osteoblast differentiation of smooth muscle cells via oxidative stress and ERK pathway.

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Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Advanced Oxidation Protein Products Induce Vascular Calcification by Promoting Osteoblastic Trans-Differentiation of Smooth Muscle Cells via Oxidative Stress and ERK Pathway

et al. 2009

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“…Several investigators have reported that the elevated expression of OPN in vasculature has a crucial role in atherosclerotic calcification [6,8,23,30]; however, the significance of whether the enhancement is inducible or preventive to the vascular calcification has not been fully revealed. In addition, recent studies have shown that, in vascular wall cells, the expression of OPN is accelerated by advanced glycation end-products [31] and mechanical stress [32]. Therefore, it might be considered that these findings can be associated with the mechanism(s) of elevating the plasma OPN level by both aging (Fig.…”

Section: Discussionmentioning

confidence: 98%

Progression of Diabetic Nephropathy Enhances the Plasma Osteopontin Level in Type 2 Diabetic Patients

et al. 2004

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Abstract. Osteopontin (OPN) is thought to play multiple roles in the progression of atherosclerotic plaque including diabetic vascular complications. However, it still remains unclear whether the level of OPN in vivo is indeed clinically associated with the progression of diabetic complications. This study evaluated whether the levels of OPN in plasma and urine are correlated with the progression of diabetic complications, such as retinopathy, neuropathy, and nephropathy in patients with type 2 diabetes. In 229 patients with type 2 diabetes, OPN level in plasma and urine was evaluated by both the severity of diabetic complications, such as retinopathy, neuropathy, and nephropathy, and the clinical characteristics and the substantial laboratory findings. Plasma OPN level increased significantly with aging and the progression of diabetic nephropathy, especially at the stage of renal failure (p<0.05). However, the level was not related to the progression of retinopathy or neuropathy, or to laboratory findings, such as HbA1c or serum lipids. In contrast, urinary OPN level was not associated with diabetic complications in any of the subjects. There was no correlation between the plasma and urinary values of OPN. The results established that the plasma OPN was elevated in proportion to the progression of diabetic nephropathy, indicating that the plasma concentration may be a potential diagnostic predictor of diabetic end-stage renal disease.

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“…In general, diabetes is thought to be a strong risk factor for the progression of not only atherosclerosis, but also arteriosclerosis. It has been reported that high glucose-induced expression of the osteopontin gene and advanced glycation end products accelerated mineralization in microvascular pericytes in a culture model 29,30) . In addition, our observations showed that patients with insulin treatment had a higher AAC grade than those without insulin in simple correlation analysis (data not shown), suggesting that the status of diabetic patients who need insulin treatment is probably more serious.…”

Section: Discussionmentioning

confidence: 99%

Validity and Usefulness of Aortic Arch Calcification in Chest X-Ray

Hashimoto

Iijima

Hashimoto

et al. 2009

J Atheroscler Thromb

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Background: Arterial calcification is associated with cardiovascular (CV) disease, to be leading to vessel wall stiffness and causing the management of hemodynamics in the elderly more difficult. Here, we compared the extent of calcification in the aortic arch by reviewing chest X-rays to that in the abdominal aorta as assessed by more detailed examinations. In addition, the validity of the grading and the relationship of this useful grading to clinical risk factors were evaluated. Methods and Results:The extent of aortic arch calcification (AAC) on a postero-anterior plain chest X-ray was divided into four grades (0 to 3). First, AAC grade was assessed in patients who underwent two quantitative examinations for abdominal aortic calcification; lateral radiograph of lumbar spine and/or computer tomography, and was positively correlated with the abdominal aortic calcification level. Subsequently, AAC grade in 239 out-patients (115 men; mean age, 61.9 years) was also evaluated, and was 0, 1, 2, and 3 in 46%, 22%, 29%, and 4% of the population, respectively, was significantly associated with pulse pressure and intima-media thickness. AAC grade in patients with diabetes or renal dysfunction was significantly higher than in those without each risk, but there was no association with other risk factors. In addition, AAC grade was positively correlated with risk factor clustering. Conclusion: Assessment of AAC detectable on a chest X-ray is very useful and its grade reflects the magnitude of calcified change in the whole aorta. In addition, AAC evaluation may provide supportive information for atherosclerotic risk stratification.

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Advanced Glycation Endproducts Accelerate Calcification in Microvascular Pericytes

Cited by 106 publications

References 15 publications

Advanced Oxidation Protein Products Induce Vascular Calcification by Promoting Osteoblastic Trans-Differentiation of Smooth Muscle Cells via Oxidative Stress and ERK Pathway

Advanced Oxidation Protein Products Induce Vascular Calcification by Promoting Osteoblastic Trans-Differentiation of Smooth Muscle Cells via Oxidative Stress and ERK Pathway

Progression of Diabetic Nephropathy Enhances the Plasma Osteopontin Level in Type 2 Diabetic Patients

Validity and Usefulness of Aortic Arch Calcification in Chest X-Ray

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