Advanced Insemination Techniques in Mares

Morris, L. H. A.

doi:10.1016/j.cveq.2006.07.001

Cited by 11 publications

(4 citation statements)

References 37 publications

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“…At 40 h after ovulation induction, the mares were inseminated using a deep horn insemination technique to deliver the 1 mL insemination dose—which contained a mean (±SEM) of 9.26 (±1.62) × 10 6 total spermatozoa in 2 × 0.5 mL straws, ipsilateral to the side of the dominant follicle or ovulation. The deep uterine insemination technique involved passing a flexible equine universal pipette (Catalogue #17209/0001; Minitube) through the cervix and manipulating it per rectum along the lumen of the uterine horn ipsilateral to the dominant follicle and depositing the insemination dose (1 mL) at the tip of the uterine horn 20 …”

Section: Methodsmentioning

confidence: 99%

“…The deep uterine insemination technique involved passing a flexible equine universal pipette (Catalogue #17209/0001; Minitube) through the cervix and manipulating it per rectum along the lumen of the uterine horn ipsilateral to the dominant follicle and depositing the insemination dose (1 mL) at the tip of the uterine horn. 20 To evaluate the fertility of the frozen-thawed, stored spermatozoa, embryos were recovered from the uterus by transcervical uterine lavage. 21,22 This was performed using a cuffed silicone catheter (French size 33) passed through the cervix into the uterine lumen.…”

Section: Mare Management and Inseminationmentioning

confidence: 99%

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A simplified fixed‐time insemination protocol using frozen–thawed stallion spermatozoa stored at 17°C for up to 24 h before insemination

Morris,

Harteveld,

Gibb

2024

Equine Veterinary Journal

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BackgroundInsemination of mares with frozen–thawed spermatozoa requires intensive management and results in 40%–60% per cycle pregnancy rates.ObjectivesTo determine if satisfactory fertility is possible for frozen–thawed semen after processing it through a microfluidic device, followed by storage at 17°C for up to 24 h before fixed‐time insemination.Study designUncontrolled field trials.MethodsA pilot study evaluated the motility of frozen–thawed spermatozoa after centrifugation and storage (17°C) in two different media for up to 48 h. Subsequently, the motility of frozen–thawed semen processed through a microfluidic device, resuspended in two different media during storage (17°C) for up to 24 h was evaluated. The fertility of frozen–thawed spermatozoa, after microfluidic sorting and storage at 17°C for up to 24 h, was evaluated after fixed‐time insemination in a commercial embryo programme. Experiment 1: Frozen–thawed spermatozoa (N = 5 stallions) were centrifuged and resuspended in Botusemen Gold™ or SpermSafe™ and stored (17°C) for up to 48 h. Sperm motility was evaluated by CASA at 0, 6, 24 and 48 h. Experiment 2: Frozen–thawed spermatozoa (N = 4 stallions) underwent microfluidic sorting and storage (17°C) for up to 24 h in both media. Sperm concentration and motility were evaluated at 0, 16 and 24 h. Experiment 3: Fertility of frozen–thawed spermatozoa (N = 3 stallions) was evaluated after insemination of 42 mare cycles at 6, 16 and 24 h after thawing, microfluidic sorting and storage before fixed‐time insemination.ResultsThe stallion significantly influenced sperm motility, but there was no effect of media on motility parameters. Storage time significantly affected sperm motility after centrifugation but not after microfluidic sorting. Storage time had no effect on the overall embryo recovery rate (52%, n = 42).Main limitationsField trial with small mare numbers and no control at time = 0 h.ConclusionsFixed‐time insemination of frozen–thawed spermatozoa after microfluidic sorting and storage at 17°C for up to 24 h produced satisfactory embryo recovery rates.

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Section: Methodsmentioning

confidence: 99%

Section: Mare Management and Inseminationmentioning

confidence: 99%

A simplified fixed‐time insemination protocol using frozen–thawed stallion spermatozoa stored at 17°C for up to 24 h before insemination

Morris,

Harteveld,

Gibb

2024

Equine Veterinary Journal

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“…Therefore, the authors stated that low-dose insemination techniques would be unlikely to improve fertility of subfertile stallions producing abnormal sperm. Morris [33] also concluded that attempts to improve the quality of frozen-thawed ejaculated or epididymal sperm by density gradient centrifugation before low-dose insemination did not benefit fertility. In another study using an aged stallion ejaculating low numbers of sperm, processing the entire ejaculate for each low-dose insemination by either centrifugation or density gradient centrifugation did not significantly improve pregnancy rates [37].…”

Section: Management Optionsmentioning

confidence: 96%

Management Options for the Aged Breeding Stallion with Declining Testicular Function

Blanchard

Varner

Love

et al. 2012

Journal of Equine Veterinary Science

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“…Benefits of sperm cryopreservation include optimizing stallion use and producing more offspring with high genetic merit. It permits the preservation of genetic material for an infinite amount of time, lowers transportation costs and hazards, reduces geographic barriers, decreases the spread of illness, and allows the use of stallions that are physically incapable of mating or have already passed away [14], and also promotes the preservation of the genetics of outstanding stallions as well as the international trade in semen [15]. Reliable freezing procedures will be required as soon as sperm sex sorting is implemented in order to optimize the usage of sex-sorted sperm in the equestrian sector [16,17].…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of stallion semen: A Review

ahmed

2023

Zagazig Veterinary Journal

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The cryopreservation of domestic animal sperms is a complicated process that necessitates the control of numerous factors in order to get the desired outcomes. Appropriate diluents and sperm dilution , cooling , and thawing rates are required to guarantee even a minimum success. To maximize the post-thaw recovery of sperm and, consequently, fertility, thorough knowledge of the intricate physiology of sperm for each species is also necessary. Cryopreserved semen is far less frequently utilized in equine artificial insemination (AI) than fresh or cooled-stored semen. This is caused, at least in part, by variations in the ability of different stallions' frozen-thawed semen to fertilize. Cryopreservation of semen is also connected with high prices. The breeding of animals has benefited greatly from the use of cryopreserved semen for artificial insemination during the past seven decades. Mammalian sperm were among the first cells to be effectively cryopreserved. The economic effectiveness of commercial artificial insemination using cryopreserved semen is therefore influenced by the number of insemination doses obtained per ejaculate, and also the one of the most challenging materials in the industry of equine semen is cryopreserving effectively without apoptosis or membrane damage.Stallions differ in their spermatozoa's cryotolerance; however, it is still unclear how much this variation is influenced by breed. Common cryopreservation methods for spermatozoa include direct submersion in liquid nitrogen and cryoprotectant-free vitrification. However, there is a chance that microbial contamination could occur with the direct freezing of sperm in commercial liquid nitrogen.So, cryopreservation aimed to protect and elongated the viability of sperms. Low-molecular-weight compounds that are very permeable are called cryoprotectants. By lowering the freezing temperature of the samples, spermatozoa are protected from freeze damage by ice crystallization. Glycerol, ethylene glycol, dimethyl sulfoxide (DMSO), and 1,2-propanediol are the four cryoprotectants that are most frequently utilaized. One of the most challenging materials in the industry of equine semen is to cryopreserve effectively without apoptosis or membrane damage. Therefore, this review explains in detail the steps of cryopreservation, its importance, and the types of cryoprotectants which added to stallion semen.

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Advanced Insemination Techniques in Mares

Cited by 11 publications

References 37 publications

A simplified fixed‐time insemination protocol using frozen–thawed stallion spermatozoa stored at 17°C for up to 24 h before insemination

A simplified fixed‐time insemination protocol using frozen–thawed stallion spermatozoa stored at 17°C for up to 24 h before insemination

Management Options for the Aged Breeding Stallion with Declining Testicular Function

Cryopreservation of stallion semen: A Review

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