Advanced instrumentation for non‐linear Raman microscopy

Yakovlev, V. V.

doi:10.1002/jrs.1090

Cited by 53 publications

(48 citation statements)

References 47 publications

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“…Further, Yakovlev et al showed that cell damage was more likely with shorter pulses and shorter near-infrared wavelengths (i.e. closer to the visible) [195]. These results were confirmed in the specific case of CARS microscopy by Fu et al [196].…”

Section: Laser Damagementioning

confidence: 84%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

270

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Laser Damagementioning

confidence: 84%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

270

189

View full text Add to dashboard Cite

show abstract

“…However, both of these arguments have to be seriously reconsidered, when implementing CARS microscopy for noninvasive imaging of biological objects. High-intensity laser pulses (1-10 kW of peak power is typically required to achieve significant level of CARS signals) can promote two-photon fluorescence and even lead to the cell's damage (15). At the same time, a high level of the CARS signal does not necessarily mean better signal-to-noise ratio because of the growing role of laser fluctuations and the presence of strong nonresonant background (14,16).…”

mentioning

confidence: 99%

“…We have recently developed a simple ''one-laser'' approach to CARS microspectroscopy, which uses a single-picosecond laser oscillator to generate a broadband continuum that is then mixed with the fundamental beam to generate a full CARS spectrum (15). The large number of already existing Ti:sapphire lasers facilitates the widespread implementation of this method (11,12,17).…”

mentioning

confidence: 99%

Comparison of coherent and spontaneous Raman microspectroscopies for noninvasive detection of single bacterial endospores

Petrov

Arora

Yakovlev

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

132

104

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Single bacterial spores were analyzed by using nonlinear Raman microspectroscopy based on coherent anti-Stokes Raman scattering (CARS). The Raman spectra were retrieved from CARS spectra and found to be in excellent agreement with conventionally collected Raman spectra. The phase retrieval method based on maximum entropy model revealed significant robustness to external noise. The direct comparison of signal amplitudes exhibited a factor of 100 stronger CARS signal, as compared with the Raman signal.microscopy ͉ nonlinear optics ͉ scattering stimulated ͉ ultrafast optics

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“…However, extensive research indicates that longer excitation wavelength provides a more gentle interaction with tissues, when ultrashort high intensity laser pulses are used. [22][23][24] In this report we focus on distinguishing different chemical species using CARS microspectroscopy, when signal is affected by scattering. The problem comes not only in the reduced signal level, but also from the fact that signal generated from different spots might experience substantial variations due to wavelengthdependent scattering both for the incident and generated beams.…”

Section: Introductionmentioning

confidence: 99%

Hyperspectral coherent anti-Stokes Raman scattering microscopy imaging through turbid medium

2011

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Abstract. Coherent Raman microspectroscopy imaging is an emerging technique for noninvasive, chemically specific optical imaging, which can be potentially used to analyze the chemical composition and its distribution in biological tissues. In this report, a hierarchical cluster analysis was applied to hyperspectral coherent anti-Stokes Raman imaging of different chemical species through a turbid medium. It was demonstrated that by using readily available commercial software (Cytospec, Inc.) and cluster analysis, distinct chemical species can be imaged and identified through a rather thick layer of scattering medium. Once the clusters of different chemical composition were distinguished, a phase retrieval algorithm was used to convert coherent anti-Stokes Raman spectra to Raman spectra, which were used for chemical identification of hidden microscopic objects. In particular, applications to remote optical sensing of potential biological threats and to imaging through a layer of skin tissue were successfully demonstrated. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Advanced instrumentation for non‐linear Raman microscopy

Cited by 53 publications

References 47 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Comparison of coherent and spontaneous Raman microspectroscopies for noninvasive detection of single bacterial endospores

Hyperspectral coherent anti-Stokes Raman scattering microscopy imaging through turbid medium

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