2021
DOI: 10.3390/biom11030397
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Advanced Killing Potential of Thymol against a Time and Temperature Optimized Attached Listeria monocytogenes Population in Lettuce Broth

Abstract: Fresh vegetables and salads are increasingly implicated in outbreaks of foodborne infections, such as those caused by Listeria monocytogenes, a dangerous pathogen that can attach to the surfaces of the equipment creating robust biofilms withstanding the killing action of disinfectants. In this study, the antimicrobial efficiency of a natural plant terpenoid (thymol) was evaluated against a sessile population of a multi-strain L. monocytogenes cocktail developed on stainless steel surfaces incubated in lettuce … Show more

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Cited by 7 publications
(4 citation statements)
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“…The MIC of each honey was determined against both S . Typhimurium and S. aureus , representing Gram-negative and Gram-positive bacteria, using the broth microdilution method, as previously described [ 16 ]. Briefly, 10 successive binary dilutions (i.e., 25–0.1% v/v ) of each honey were prepared using TSB as the diluent.…”
Section: Methodsmentioning
confidence: 99%
“…The MIC of each honey was determined against both S . Typhimurium and S. aureus , representing Gram-negative and Gram-positive bacteria, using the broth microdilution method, as previously described [ 16 ]. Briefly, 10 successive binary dilutions (i.e., 25–0.1% v/v ) of each honey were prepared using TSB as the diluent.…”
Section: Methodsmentioning
confidence: 99%
“…Each Campylobacter isolate (n = 107; since eight C. jejuni and five C. coli isolates failed to be resuscitated following their cryostorage for us to be able to re-extract their gDNA for the rep-PCRs) was subtyped below the species level following a previously described rep-PCR approach [36], with some minor adaptations. Briefly, Kapa Taq PCR Kit with dNTPs was used for the PCRs.…”
Section: Rep-pcr and Phylogenetic Discrimination Of The Isolatesmentioning
confidence: 99%
“…The strain of S. enterica belongs to serovar Typhimurium; it is an epidemic strain of phage type DT193 and was originally isolated from a human salmonellosis outbreak [25]. The strain of L. monocytogenes belongs to serovar 1/2b and was originally isolated from a mixed green salad [26]. Before use, both strains were maintained frozen at −80 • C in BHI broth (Lab M, Heywood, Lancashire, UK) containing 15% v/v glycerol.…”
Section: Bacterial Strains and Preparation Of Their Working Culturesmentioning
confidence: 99%