Advanced methods to mechanically isolate stromal vascular fraction: A concise review

You, Xin; Gao, JianHua; Yao, Yao

doi:10.1016/j.reth.2024.03.020

Cited by 3 publications

(1 citation statement)

References 39 publications

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“…The Adinizer, a patented double-sided ultrasharp blade system, has been used in many different studies in the literature to obtain stromal cells from fat tissue and to apply different sizes to different anatomical areas in fat tissue grafts, and successful results have been reported. [11][12][13][14][15][16][17][18][19] The process of mechanically obtaining stromal cells using the Adinizer is called mechanical stromal cell transfer, whereas the creation of fat tissue in different sizes is called adjustable regenerative adipose transfer. 6 This approach has enabled the application of different protocols for different indications, and indication-based protocols have been presented in the literature for the first time.…”

Section: Discussionmentioning

confidence: 99%

Autologization of Exosomes with Deparenchymized Adipose Tissue: An Innovative Approach for Regenerative Medicine and Surgery

Copcu

2024

Plastic and Reconstructive Surgery - Global Open

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Background: Among all regenerative applications developed in recent years, the use of exosomes has generated by far the greatest interest. Exosome products from allogeneic and xenogeneic sources are available on the market. A key challenge is controlling the effects of nonautologous exosomes. We hypothesized that combining exosomes with a patient’s own extracellular matrix (ECM) can create “autologization,” enabling control over their effects. This study aimed to provide the rationale and a guide for future research exploring the autologization of exosome applications using deparenchymized adipose tissue (DPAT). Methods: DPAT adipose tissue was achieved using 1200-, 400-, and 35-micrometer blades in an ultrasharp blade system (Adinizer), and then “autologization” was achieved by combining the obtained DPAT with allogeneic exosomes. DPAT was evaluated histochemically, and exosomes were counted and analyzed with the Nanosight device. Results: The DPAT process using ultrasharp blades is easily performed. DPAT obtained from adipose tissue was then combined with allogenic exosomes. It has been demonstrated histopathologically that adipocytes are eliminated in deparenchymized fat tissue, and only ECM and stromal cells remain. It has also been proven that the number of exosomes is not affected by the combination. Conclusions: This study introduces two novel concepts previously unknown in the literature, “deparenchymization” and “autologization,” representing an innovative approach in plastic surgery and regenerative medicine. Our novel approach enriches regenerative cells while preserving critical ECM signals, overcoming the limitations of existing isolation methods. Extensive research is still needed, but autologization using DPAT ECM holds great promise for translating exosome-based treatments into practice.

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Section: Discussionmentioning

confidence: 99%

Autologization of Exosomes with Deparenchymized Adipose Tissue: An Innovative Approach for Regenerative Medicine and Surgery

Copcu

2024

Plastic and Reconstructive Surgery - Global Open

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Biological attributes required for epidermal regeneration: Evaluation of the next‐generation autologous cell harvesting device

Bush,

Kashgari,

Jahid

et al. 2024

International Wound Journal

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Early wound intervention and closure is critical for reducing infection and improving aesthetic and functional outcomes for patients with acute burn wounds and nonthermal full‐thickness skin defects. Treatment of partial‐thickness burns or full‐thickness injuries with autologous skin cell suspension (ASCS) achieves robust wound closure while limiting the amount of donor skin compared with standard autografting. A Next Generation Autologous Cell Harvesting Device (NG‐ACHD) was developed to standardize the preparation process for ASCS to ensure biological attributes are obtained known to correlate with well‐established safety and performance data. This study compared ASCS prepared using the NG‐ACHD and ACHD following the manufacturer's guidance, evaluating cellular yields, viability, apoptotic activity, aggregates, phenotypes and functional capacity. Non‐inferiority was established for all biological attributes tested and comparable healing trajectories were demonstrated using an in vitro skin regeneration model. In addition to standardization, the NG‐ACHD also provides workflow efficiencies with the potential to decrease training requirements and increase the ease of incorporation and utilization of ASCS in clinical practice.

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Evaluation of a Novel Mechanical Device for the Production of Microfragmented Adipose Tissue for Veterinary Regenerative Medicine: A Proof-of-Concept

Berni,

Andreoli,

Conti

et al. 2024

IJMS

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Therapies based on mesenchymal stromal cells (MSCs) have become one of the most significant advancements in veterinary regenerative medicine. The isolation of MSCs is usually performed by enzymatic digestion and requires variable times for cell expansion. In addition, these procedures need to be performed in specialized laboratory facilities. An alternative approach to in vitro-expanded MSC therapy is the use of \microfragmented adipose tissue (microfat), which is a rich source of cells and growth factors from the stromal vascular fraction. Recent clinical studies support its safety and efficacy in the treatment of musculoskeletal disorders and wound healing. The aim of the present work was to characterize the microfragmented adipose tissue obtained by a new mechanical device, which provides sterile tissue that is ready for use in the clinic by the veterinarian, avoiding the need for specialized laboratory facilities. Microfat-derived MSCs were compared with enzymatically isolated MSCs in terms of their phenotypic characterization, growth rate and differentiation potential. Conditioned medium derived from microfat culture was evaluated for its ability to promote MSC vitality. No differences were observed between MSCs obtained through mechanical fragmentation and those derived from collagenase digestion of adipose tissue, suggesting that the device could serve as a practical source of microfragmented adipose tissue for use in veterinary clinics.

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Advanced methods to mechanically isolate stromal vascular fraction: A concise review

Cited by 3 publications

References 39 publications

Autologization of Exosomes with Deparenchymized Adipose Tissue: An Innovative Approach for Regenerative Medicine and Surgery

Autologization of Exosomes with Deparenchymized Adipose Tissue: An Innovative Approach for Regenerative Medicine and Surgery

Biological attributes required for epidermal regeneration: Evaluation of the next‐generation autologous cell harvesting device

Evaluation of a Novel Mechanical Device for the Production of Microfragmented Adipose Tissue for Veterinary Regenerative Medicine: A Proof-of-Concept

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