Advanced multi-modal, multi-analyte optochemical sensing platform for cell analysis

Zhdanov, Alexander V.; Li, Liang; Yang, Panpan; Shkirdova, Alena O.; Tang, Shuze; Yashunsky, Dmitry V.; Ponomarev, G. V.; Zamilatskov, Ilya A.

doi:10.1016/j.snb.2021.131116

Cited by 9 publications

(8 citation statements)

References 37 publications

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“…Nevertheless, by incorporating using a second spectrally distinct coumarin C6 dye (O2 and pH insensitive), the referenced ratiometric intensity based pH sensing was implemented (Fig. 3B), but it still requires correction for the cross-sensitivity with O2 [13]. Figures were adopted from [13].…”

Section: Alternative Sensing Platformsmentioning

confidence: 99%

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Optochemical sensor-based systems for the analysis of cell metabolism and bioenergetics: an overview

Papkovsky

2023

Optical Sensors 2023

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Optochemical sensor based systems for analysis of cell metabolism are well established and continue to grow providing valuable and detailed information on cell bioenergetics, metabolic signature and ability to withstand stress conditions and drug treatments. The area is currently dominated by the Seahorse/Agilent XF (eXtracellular Flux) Analyzer and Luxcel/Agilent MitoXpress platforms, which provide measurement of Oxygen Consumption (OCR) and Extracellular Acidification (ECA) rates under different physiological conditions (metabolic substrates, drugs, stressors), by means of fluorescent and phosphorescent pH and O2 sensors or probes. The XF system uses solid-state sensors and specialized microplates, it is highly integrated, sensitive and fast, and user-friendly. However, the outdated sensor chemistry and basic fluorescence intensity readouts limit its performance (unstable sensor calibrations, cross-talk) and make it expensive. The MitoXpress platform, which uses soluble probes, standard microplates and plate readers, provides stable and accurate lifetime based sensing of O2 and pH with internal referencing and no cross-talk. However, its cost-efficient DIY (do it yourself) approach is less sensitive and less user-friendly than the XF. Several new dual pH/O2 sensing platforms are emerging, which can potentially improve the above systems and overcome their limitations. Examples include: i) a meso-substituted Pt-porphyrin Schiff base dye, which senses pH and O2 via phosphorescence intensity and lifetime changes; ii) the fully-referenced solid-state dual sensor based on a pH-sensitive fluorescent porphyrin and an O2-sensitive phosphorescent Pt-porphyrin. iii) a sensor with near-infrared fluorescent pH-indicator and phosphorescence O2 indicator dyes and phase measurements at multiple frequencies. In this paper, we discuss the merits and limitations of the different sensor systems for cell analysis.

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Section: Alternative Sensing Platformsmentioning

confidence: 99%

“…3B), but it still requires correction for the cross-sensitivity with O2 [13]. Figures were adopted from [13].…”

Section: Alternative Sensing Platformsmentioning

confidence: 99%

Optochemical sensor-based systems for the analysis of cell metabolism and bioenergetics: an overview

Papkovsky

2023

Optical Sensors 2023

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“…1,2 Current methods for the quantitative detection of cells mainly include enzyme-linked immunosorbent assays, polymerase chain reaction, and flow cytometry-based techniques. 3 Unfortunately, these well-established approaches have obvious drawbacks of complicated instrumentation and intensive labor procedures. 4 In addition, the antibody activity of immunoassays is not easy to maintain and natural antibodies are difficult to purify.…”

Section: Introductionmentioning

confidence: 99%

“…CIPs are synthetic polymer materials that capture the structural and chemical information of cells through template-assisted assembly of functional groups. 3 Typically, a polymer is cured around template cells that are removed subsequently, leaving empty holes which are complementary in size, shape, and functionality to the target cells. 5 On account of their unique advantages such as physicochemical resistance, low cost, reusability, and ease of processing, CIPs have been attracting more and more interest.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of high-performance cell-imprinted polymers based on AuNPs/MXene composites via metal-free visible light-induced ATRP

Cui

Meng

et al. 2023

Analyst

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“…Physiological pH values normally range between 6.0 and 8.0, spanning down to pH 4-6 for some specialized cellular compartments) [1]. Determination of pH values in biological fluids, cells and tissue samples is important for medicine, biotechnology, tumor radiotherapy, corrosion monitoring, food and chemical sciences [2][3][4]. The gold standard of pH analysis is glass pH electrode, which can provide rapid, stable, and reliable readout in a wide pH range, which is proportional to sample pH.…”

Section: Introductionmentioning

confidence: 99%

New solid-state optochemical pH sensors for the analysis of cell bioenergetics

Zhdanov

2023

Optical Sensors 2023

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Monitoring pH and extracellular acidification in biological samples containing live mammalian cells can provide valuable information on the glycolytic activity and bioenergetic status of cells. Compared to pH electrodes, optochemical pH sensors look more advantageous, since they allow rapid, non-invasive parallel analysis of multiple samples with stable readout of pH. We have developed new fluorescent pH sensors based on hydrophobic protonable metal-free porphyrins (OEP and OEPK) embedded in a proton-permeable polymeric matrix together with a proton transfer agent. These pH sensors provide internally-referenced calibration-free operation, both in ratiometric intensity and lifetime based detection modes. Sensor development included optimization of the indicator dye and its photophysical characteristics, screening of different proton transfer agents to minimize sensor toxicity, tuning of protonation range and pKa, long-term storage stability and response time studies. Optimised pH sensor coatings were then deposited on plastic substrates (96-well microplates) and used for real-time monitoring of Extracellular Acidification Rate (ECAR) for cultured cancer cells and 3D spheroid structures on standard laboratory equipment (multi-label plate reader and confocal FLIM microscope). The advanced pH sensors tailored for use with biological samples have high potential for cell analysis and related applications.

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Advanced multi-modal, multi-analyte optochemical sensing platform for cell analysis

Cited by 9 publications

References 37 publications

Optochemical sensor-based systems for the analysis of cell metabolism and bioenergetics: an overview

Optochemical sensor-based systems for the analysis of cell metabolism and bioenergetics: an overview

Fabrication of high-performance cell-imprinted polymers based on AuNPs/MXene composites via metal-free visible light-induced ATRP

New solid-state optochemical pH sensors for the analysis of cell bioenergetics

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