Metallic microstructures are the essential building blocks in microelectronics as electric interconnection between different functioning parts. [1] In the blooming field of optoelectronics, metallic microstructures also play a key role due to surfaceplasmon-plariton-related effects, [2,3] applications in miniaturization of photonic circuits, [4,5] near-field optics, [6] and singlemolecule optical sensing. [7,8] The electromagnetic resonance in metallic microstructures may offer unique properties that do not exist in natural materials, such as negative refractive index. [9,10] In previous studies, metallic microstructures were usually fabricated by photolithography, which was time-consuming and costly. [11] Template-assisted electrodeposition is an easy way to fabricate microstructures. For example, anodic aluminum oxide (AAO) [12][13][14] and polymeric membranes [15] have been used as molds, and the size of the channels in these systems can reach a few nanometers.[16] However, the destructivity in removal template renders it difficult to preserve the spatial order among the nanowires, [17,18] hence limits their applications in optoelectronics. We once introduced a selective electrodeposition method to fabricate two-dimensional metallic structures, where the substrate surface was modified by stripes of lipid monolayers, on which nucleation of metal crystallites was easier.[19] However, in that case the width of the metallic wires was limited by the geometrical shape of templates, that is, the width of metallic wires could not be tuned unless a new template was used. Furthermore, previously fabricated wires [19] were essentially flat belts lying on the substrate, which would not be effective if applied to sensors where a large specific surface area is usually expected. [20] Therefore, one of the important challenges in fabricating metallic microstructures is to find an easy, repeatable, and controllable method to meet the increasing demands in optoelectronics and plasmonics.In this communication, we report a new template-assisted electrochemical approach to fabricate arrays of metallic nanowires. Unlike conventional template-assisted growth, where the generated wires are confined by the size of template, in our case the width of the metallic wires can be tuned by changing the control parameters of electrodeposition. By imprinting polymer stripes on a silicon surface, the concave corner of polymer stripes and silicon substrate provides a preferential nucleation site for the formation of metal nanowires. The width of wires can be tuned from 25 nm to a few hundred nanometers. Further, we demonstrate that this method can be applied for fabricating more complicated structures rather than straight lines only.In our experiments, the metallic nanowires are electrodeposited with the help of polymer stripes embossed on silicon surfaces. To form the patterned substrate, a thin film of poly(methylmethacrylate) (PMMA) (mr-I 7030E M w ¼ 75 kDa) is initially spin-coated on the silicon wafer. The film thickness is about 300 nm. Th...