Advanced PHB fermentation strategies with CO2-derived organic acids

Vlaeminck, Elodie; Quataert, Koen; Uitterhaegen, Evelien; Winter, Karel De; Soetaert, Wim

doi:10.1016/j.jbiotec.2021.11.010

Cited by 21 publications

(27 citation statements)

References 32 publications

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“…Gas fed to the reactors result in mass transfer to the liquid phase according to: where k L a i us the volumetric mass-transfer coefficient on the liquid side of the gas/liquid interface and β n,i is the Bunsen solubility coefficient. We assume is 300 hr −1 following our previous analysis 5 , which represents an intermediate value of the range observed in standard bioreactors 6,7 . To calculate , we use: where and are the diffusivities of O 2 and CO 2 following Meraz et al 57 to account for the differences in the mass transfer coefficient 8 .…”

Section: Methodsmentioning

confidence: 99%

“…Species of the bacterial genus Cupriavidus exhibit the highest productivities of natural or recombinant poly-hydroxybutyrate (PHB) producing organisms 4 . While it is possible to attain very high PHB productivities using a variety of organisms through sophisticated multistage bioreactor or feeding regimes [5][6][7] , these systems can require very high substrate loadings, suffer from low overall substrate utilization, and necessitate regular shutdown and start-up cycles in-between batches 4,5,8,9 . Maintaining high substrate utilization remains important for both economically viable industrial bioplastic production 2 and in resource-constrained, remote and austere bioplastic production and deployment settings 10 .…”

Section: Introductionmentioning

confidence: 99%

“…(34) collapses to 𝐷 For the gas phase(s), we define a feed term according to: -phase dilution rate in reactor . 𝐷 𝑔𝑎𝑠,𝑛𝑛Gas-liquid mass transferGas fed to the reactors result in mass transfer to the liquid phase according to: mass-transfer coefficient on the liquid side of the gas/liquid 𝑘 which represents an intermediate value of the range observed in standard bioreactors6,7 . To calculate , we use: of O 2 and CO 2 following Meraz et al57 to account for the 𝐷 𝑂 in the mass transfer coefficient8 .We calculate the equilibrium solubility of O 2 and CO 2 according to the empirical relationship for the Bunsen solubility coefficient ( ):…”

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confidence: 99%

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Eliminating Genes for a Two Component System Increases PHB Productivity in Cupriavidus basilensis 4G11 Under PHB Suppressing, Non-Stress Conditions

Sander

Abel

Friedline

et al. 2022

Preprint

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Species of bacteria from the genus Cupriavidus are known, in part, for their ability to produce high amounts of poly-hydroxybutyrate (PHB) making them attractive candidate bioplastic producers. The native production of PHB occurs during periods of metabolic stress, and the process regulating the initiation of PHB accumulation in these organisms is not fully understood. Screening an RB-TnSeq transposon library of Cupriavidus basilensis 4G11 allowed us to identify two genes of an apparent, uncharacterized two component system which, when omitted from the genome, are capable of increased PHB productivity in balanced, non-stress growth conditions. We observe average increases in PHB productivity of 56% and 41% relative to the wildtype parent strain, upon deleting each of two genes individually from the genome. The increased PHB phenotype disappears, however, in nitrogen-free unbalanced growth conditions suggesting the phenotype is specific to fast-growing, replete, non-stress growth. Bioproduction modeling suggests this phenotype could be due to a decreased reliance on metabolic stress induced by nitrogen limitation to initiate PHB production in the mutant strains. Such strains may allow for the use of single stage, continuous bioreactor systems, which are far simpler than PHB bioproduction schemes used previously. Bioproductivity modeling suggests that omitting this regulation in the cells may increase PHB productivity up to 24% relative to the wildtype organism when using single stage continuous systems. This work furthermore expands our understanding of the regulation of PHB accumulation in Cupriavidus, in particular the initiation of this process upon transition into unbalanced growth regimes.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Eliminating Genes for a Two Component System Increases PHB Productivity in Cupriavidus basilensis 4G11 Under PHB Suppressing, Non-Stress Conditions

Sander

Abel

Friedline

et al. 2022

Preprint

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“…Alternatively, recent research has highlighted the potential of two-step, electrocatalysis and biocatalysis pathways to convert CO 2 to PHB through an intermediate carbon carrier, avoiding the identified barriers of using CO 2 directly. 33,34 These pathways also provide the benefit of process flexibility through the ability to produce PHB from one or more chemical intermediates.…”

Section: Current Reality and Future Potentialmentioning

confidence: 99%

Designing an innovation system to support profitable electro- and bio-catalytic carbon upgrade

Ruttinger

Tavakkoli

Shen

et al. 2022

Energy Environ. Sci.

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“…In this respect, the development of novel synthetic routes enabling direct formate incorporation could offer a solution, while also improving PHB production (Claassens et al 2020 ). Alternatively, better results have been obtained using acetic acid, amounting to 79 g/L biomass containing 74% PHB at 0.93 g/(L∙h) PHB, thanks to its more efficient metabolization towards PHB (Table 1 ) (Vlaeminck et al 2022 ). Since acetic acid can also efficiently be produced from CO 2 and CO by acetogenic bacteria in an anaerobic gas fermentation, this gives rise to an interesting two-stage fermentation.…”

Section: Phb Production From Industrial C1 Gas Emissionsmentioning

confidence: 99%

Industrial side streams as sustainable substrates for microbial production of poly(3-hydroxybutyrate) (PHB)

Vlaeminck

Uitterhaegen

Quataert

et al. 2022

World J Microbiol Biotechnol

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Poly(3-hydroxybutyrate) (PHB) is a microbially produced biopolymer that is emerging as a propitious alternative to petroleum-based plastics owing to its biodegradable and biocompatible properties. However, to date, the relatively high costs related to the PHB production process are hampering its widespread commercialization. Since feedstock costs add up to half of the total production costs, ample research has been focusing on the use of inexpensive industrial side streams as carbon sources. While various industrial side streams such as second-generation carbohydrates, lignocellulose, lipids, and glycerol have been extensively investigated in liquid fermentation processes, also gaseous sources, including carbon dioxide, carbon monoxide, and methane, are gaining attention as substrates for gas fermentation. In addition, recent studies have investigated two-stage processes to convert waste gases into PHB via organic acids or alcohols. In this review, a variety of different industrial side streams are discussed as more sustainable and economical carbon sources for microbial PHB production. In particular, a comprehensive overview of recent developments and remaining challenges in fermentation strategies using these feedstocks is provided, considering technical, environmental, and economic aspects to shed light on their industrial feasibility. As such, this review aims to contribute to the global shift towards a zero-waste bio-economy and more sustainable materials.

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Advanced PHB fermentation strategies with CO2-derived organic acids

Cited by 21 publications

References 32 publications

Eliminating Genes for a Two Component System Increases PHB Productivity in Cupriavidus basilensis 4G11 Under PHB Suppressing, Non-Stress Conditions

Eliminating Genes for a Two Component System Increases PHB Productivity in Cupriavidus basilensis 4G11 Under PHB Suppressing, Non-Stress Conditions

Designing an innovation system to support profitable electro- and bio-catalytic carbon upgrade

Industrial side streams as sustainable substrates for microbial production of poly(3-hydroxybutyrate) (PHB)

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