Advanced Recombinant and Regenerated Silk Materials for Medicine and Tissue Engineering

Тенчурин, Т. Х.; Шариков, Р. В.; Чвалун, С. Н.

doi:10.1134/s1995078019040128

Cited by 5 publications

(4 citation statements)

References 50 publications

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“…8). After the phase of degumming, we can dissolve the silk fiber with a salt solution followed by dialysis to obtain an aqueous silk solution; the silk solution is formed [36,37]. For corneal engineering, centrifugal casting produces fit silk fibroin films [38].…”

Section: B) Regenerated Silkmentioning

confidence: 99%

The Silk, Versatile Material for Biological, Optical, and Electronic Fields: Review

Bibbò¹,

Khan²,

Tareen³

2021

GJRE

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Silk, seen as a material, is a fiber made from silkworm cocoons and spiders. They have standard structural components and hierarchical structures. Different manufacturing techniques allow obtaining silk in films, fibers, hydrogels, microspheres, and sponges. We can tune the properties through the structure of secondary proteins. The paper explores the application in biomedical, optics, and electronic fields by analyzing the technological trend.

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Section: B) Regenerated Silkmentioning

confidence: 99%

The Silk, Versatile Material for Biological, Optical, and Electronic Fields: Review

Bibbò¹,

Khan²,

Tareen³

2021

GJRE

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“…The RS-based matrices are currently successfully used as cell scaffolds for the proliferation and differentiation of various types of cells [ 9 , 10 ]. The fibrous materials fabricated by electrospinning from the RS solutions have a structure and mechanical properties that mimic the natural extracellular matrix [ 11 , 12 ], which is of considerable interest for tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

“…RS usually contain a large number of primary repeats characteristic of the central part of natural spidroins, but they have a lower molecular weight. Because the N- and C-terminal sequences are absent in most RS, they have very low solubility in water, i.e., from 0.4 up to 2% [ 12 ]. Therefore, for the dissolving RS, organic solvents are mainly used, e.g., hexafluoroisopropanol (HFIP).…”

Section: Introductionmentioning

confidence: 99%

Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials

et al. 2023

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The effect of primary amino acid sequence in recombinant spidroins on their spatial organization is crucial for the fabrication of artificial fibers and fibrous materials. This study focuses on the rheological properties of aqueous and alcoholic solutions of recombinant analogs of natural spidroins (rS1/9 and rS2/12), as well as the structure of their films and nanofibrous materials. Non-Newtonian flow behavior of aqueous solutions of these proteins was observed at certain concentrations in contrast to their solutions in hexafluoroisopropanol. The secondary structure of recombinant spidroins was addressed by IR spectroscopy, whereas their self-organization in various solvents was studied by AFM and cryo-TEM. The influence of the solvent on the structure and properties of the films and nanofibrous materials produced by electrospinning has been established.

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“…Substance secreted from spider's major ampullate glands attracts scientific attention for many decades due to its unique rheological and biochemical properties. [ 1,2 ] Dragline silk is formed after the hardening of the spinning dope and consists of two different proteins : MaSp1 and MaSp2 with molecular weights around 300 kDa. Despite the similarity of their amino acid sequences there are a number of differences in their functional properties, MaSp2 containing proline residues reveals higher elasticity for example.…”

Section: Introductionmentioning

confidence: 99%

Effect of Shear on Structural Organization of Various Recombinant Spidroins

Шариков

Тенчурин

Белоусов

et al. 2022

Macromolecular Symposia

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To follow the structural reorganization of spidroin during its spinning, the rheological behavior of low‐concentration solution of recombinant spidroins rS1/9 and rS2/12 is studied on different stages of separation, purification, and lyophilization. It is shown that spidroin solutions with concentration of 1 mg mL−1 appear to be structured liquids with yield stress around value 0.2 Pa. Distribution of hydrodynamic radii of spidroins reveals two peaks due to presence of single particles and aggregates both. Their ability for spidroin transformation from micellar to fibrillar structure under shear stress is demonstrated. Studying rheological behavior of recombinant spidroin solutions and their supramolecular organization is necessary to select the optimal spinning parameters to produce fibrous materials with required physicochemical properties.

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Advanced Recombinant and Regenerated Silk Materials for Medicine and Tissue Engineering

Cited by 5 publications

References 50 publications

The Silk, Versatile Material for Biological, Optical, and Electronic Fields: Review

The Silk, Versatile Material for Biological, Optical, and Electronic Fields: Review

Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials

Effect of Shear on Structural Organization of Various Recombinant Spidroins

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