Advanced Sperm Processing/Selection Techniques

Agarwal, Ashok; Selvam, Manesh Kumar Panner

doi:10.1007/978-3-319-71815-6_28

Cited by 4 publications

(2 citation statements)

References 62 publications

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“…This selection method should consider determinants such as progressive motility, morphology, DNA integrity and maturity of spermatozoa for successful pregnancy outcomes. [ 1 2 ] It has been suggested that the sperm selection method which can mimic the natural selection process of spermatozoa in the female reproductive tract for fertilisation will result in a better population of spermatozoa for ART.…”

Section: Introductionmentioning

confidence: 99%

Does choosing microfluidics for sperm sorting offer an advantage to improve clinical pregnancies in donor egg recipients?

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Does choosing microfluidics for sperm sorting offer an advantage to improve clinical pregnancies in donor egg recipients?

et al. 2022

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“…Excessive presence of leucocytes (>10 6 leucocytes/ml semen) in semen is known as leucocytospermia (Wallach & Wolff, ). Conventional sperm processing techniques such as density gradient and swim‐up can be used to remove the round cells, debris and leucocytes from semen samples (Agarwal & Selvam, ; Beydola, Sharma, Lee, & Agarwal, ). However, most of the sperm tests are performed on neat semen samples, including advanced sperm function tests to assess sperm DNA fragmentation, oxidative stress and sperm fertilising ability (Agarwal & Said, ; Kızılay & Atlay, ; Majzoub, Esteves, Gosálvez, & Agarwal, ).…”

Section: Introductionmentioning

confidence: 99%

Round cells do not contaminate or mask human sperm proteome in proteomic studies using cryopreserved samples

et al. 2019

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Semen contains leucocytes and round cells, besides spermatozoa. The objective of this study was to identify whether the proteins from round cells and leucocytes affect the proteomic analysis of spermatozoa. Cryopreserved human sperm samples were divided into four groups: (1) samples with ≥1 × 106/ml leucocytes unprocessed; (2) samples with ≥1 × 106/ml leucocytes processed by 65% density centrifugation; (3) samples with round cells <1 × 106/ml unprocessed; and (4) samples with round cells <1 × 106/ml processed by 65% density centrifugation. Samples from each group (1, 2, 3 and 4) were pooled (n = 5) for quantitative proteomic analysis using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). Comparative analysis revealed nine differentially expressed proteins (DEPs) groups 1 and 2. Moreover, five DEPs were identified between groups 3 and 4. We observed that cylicin‐1, Atlastin‐1 and vesicle transport protein SFT2B are specific to spermatozoa, and none of them were associated with leucocytes. The number of DEPs in spermatozoa of processed and unprocessed cryopreserved semen samples was negligible. Our results indicate that the presence of round cells (<1 × 106/ml) in the seminal ejaculation does not interfere in the accurate detection of spermatozoa proteome by LC‐MS/MS.

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The influence of the female reproductive tract and sperm features on the design of microfluidic sperm-sorting devices

Ahmadkhani

Hosseini

Saadatmand

et al. 2022

J Assist Reprod Genet

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Advanced Sperm Processing/Selection Techniques

Cited by 4 publications

References 62 publications

Does choosing microfluidics for sperm sorting offer an advantage to improve clinical pregnancies in donor egg recipients?

Does choosing microfluidics for sperm sorting offer an advantage to improve clinical pregnancies in donor egg recipients?

Round cells do not contaminate or mask human sperm proteome in proteomic studies using cryopreserved samples

The influence of the female reproductive tract and sperm features on the design of microfluidic sperm-sorting devices

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