Advanced Strategies in Bulked Segregant Analysis and its Applications

Abstract

Bulked segregant analysis (BSA) is a technique used to identify genetic markers associated with a mutant phenotype and is a quick method for identifying markers in particular genome regions. The paper focussed on Advanced methods which escape the requirement of genotyping all the individuals of the mapping population and generation of high-density linkage maps for mapping of the gene for the trait of interest. With the emergence of re-sequencing techniques, quick mapping of genes has become possible with reduced time and cost by using advanced methodologies like MutMap, MutMap+, MutMap-Gap, QTL-Seq, RNAseq BSA, NGS BSA and QTG seq. The procedure for various advanced BSA strategies has been described.

An Overview on Phenomics Applications in Different Agriculture Disciplines

“…These methods find pertinent data for use in plant breeding and pathology activities by using feature extraction, identification, classification, and prediction criteria as well as for precision breeding. The use of these helps to speed up genetic progress [3] and lessen the phenotyping and genotyping gap. The various applications of phenomics approach used by researchers are listed in the present study.…”