Advanced Techniques to Study Anesthetic Effects on the Nervous System

Wang, Cheng; Carmona, Maria José Carvalho

doi:10.17352/2455-3476.000023

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…Fallypride is a high-affi nity dopamine D2/D3 receptor antagonist [23][24][25]. As a minimally invasive, dynamic and leading approach, PET imaging can acquire quantitative images from living animal organs such as the brain [26,27]. Since PET imaging data can be collected from the same subject at multiple time points, it provides the possibility of continuously examining physiological and pharmacological processes of interesting targets in vivo.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Dopamine Receptor Integrity after Sevofl urane Exposure in Neonatal Rat Brain Using Positron Emission Tomography

Yin¹,

Paule²,

Patterson³

et al. 2018

Glob J Anesth

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Aims: The volatile general anesthetic sevofl urane is commonly used across all ages in the clinic. Sevofl urane-induced neurotoxic effects on the developing dopaminergic system are still unclear. The aim of this study was to evaluate the integrity of the D2/D3 receptor in developing rat brain utilizing molecular imaging techniques. Method: Positron Emission Tomography (PET) coupled with Computerized Tomography (CT) approaches were used to evaluate the effects of developmental sevofl urane exposure on D2/D3 receptors in rat pups. The PET radiotracer, [ 18 F]-fallypride, was used to examine whether dopamine receptors were affected by prolonged sevofl urane-exposure during the brain growth spurt. Six postnatal day (PND) 7 rats in the experimental group were exposed to sevofl urane at the clinically-relevant concentration of 2.5% (v/v) for 8 hours, and an equal number of control animals were exposed to room air for 8 hours. MicroPET/ CT scans were sequentially collected on PNDs 14, 21, 28, and 35. Results: No signifi cant differences in the retention of [ 18 F]-fallypride in striatum were detected between sevofl urane-exposed and control animals at any of the scan times. D2/D3 receptors are not affected by prolonged sevofl urane-induced general anesthesia during development. Conclusions: Sevofl urane-induced neurotoxicity in the developing rodent brain was not associated with apparent derangements in dopamine D2/D3 receptors.

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