Advancement and applications of peptide phage display technology in biomedical science

Wu, Chien‐Hsun; Liu, I-Ju; Lu, Ruei‐Min; Wu, Han‐Chung

doi:10.1186/s12929-016-0223-x

Cited by 286 publications

(194 citation statements)

References 149 publications

(210 reference statements)

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“…To obtain highaffinity targets, up to five rounds of biopanning are performed (Figure 5). Finally, DNA sequencing of the primary structure is carried out to produce individual clones of the target protein (Bazan et al, 2012;Wu et al, 2016).…”

Section: Production Methodsmentioning

confidence: 99%

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Antibody Engineering for Pursuing a Healthier Future

et al. 2017

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Since the development of antibody-production techniques, a number of immunoglobulins have been developed on a large scale using conventional methods. Hybridoma technology opened a new horizon in the production of antibodies against target antigens of infectious pathogens, malignant diseases including autoimmune disorders, and numerous potent toxins. However, these clinical humanized or chimeric murine antibodies have several limitations and complexities. Therefore, to overcome these difficulties, recent advances in genetic engineering techniques and phage display technique have allowed the production of highly specific recombinant antibodies. These engineered antibodies have been constructed in the hunt for novel therapeutic drugs equipped with enhanced immunoprotective abilities, such as engaging immune effector functions, effective development of fusion proteins, efficient tumor and tissue penetration, and high-affinity antibodies directed against conserved targets. Advanced antibody engineering techniques have extensive applications in the fields of immunology, biotechnology, diagnostics, and therapeutic medicines. However, there is limited knowledge regarding dynamic antibody development approaches. Therefore, this review extends beyond our understanding of conventional polyclonal and monoclonal antibodies. Furthermore, recent advances in antibody engineering techniques together with antibody fragments, display technologies, immunomodulation, and broad applications of antibodies are discussed to enhance innovative antibody production in pursuit of a healthier future for humans.

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Section: Production Methodsmentioning

confidence: 99%

“…Likewise, they have better fusion in bacteria, and display on a filamentous phage. These fragments permit the production of homogenous proteins for diagnostic and therapeutic purposes as well as structural studies (Frenzel et al, 2013;Wu et al, 2016).…”

Section: Single-chain Variable Fragment (Scfv)mentioning

confidence: 99%

Antibody Engineering for Pursuing a Healthier Future

et al. 2017

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“…This combination of signals can be a practical strategy to mimic the complex extracellular microenvironment that regulates stem cell behavior. Aside from selecting specific amino acid domains that have been discovered in natural ECM proteins, synthetic peptides with cell-mediating functions have also been identified through phage display technology [43] . Phage display is a powerful tool that enables the high throughput identification of peptides, activate specific receptors that regulate cell adhesion, differentiation as well as those that bind to specific cell types.…”

Section: Strategies To Achieve Stem and Progenitor Cell Recruitmentmentioning

confidence: 99%

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Pacelli

Basu

Whitlow

et al. 2017

Advanced Drug Delivery Reviews

132

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A leading strategy in tissue engineering is the design of biomimetic scaffolds that stimulate the body’s repair mechanisms through the recruitment of endogenous stem cells to sites of injury. Approaches that employ the use of chemoattractant gradients to guide tissue regeneration without external cell sources are favored over traditional cell-based therapies that have limited potential for clinical translation. Following this concept, bioactive scaffolds can be engineered to provide a temporally and spatially controlled release of biological cues, with the possibility to mimic the complex signaling patterns of endogenous tissue regeneration. Another effective way to regulate stem cell activity is to leverage the inherent chemotactic properties of extracellular matrix (ECM)-based materials to build versatile cell-instructive platforms. This review introduces the concept of endogenous stem cell recruitment, and provides a comprehensive overview of the strategies available to achieve effective cardiovascular and bone tissue regeneration.

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“…for binding or functional activities [14], or released in situ for solution phase functional assays [36]. Phage-display peptide libraries can be screened with bio-panning [37]or limited cell-based functional assays, such as cell-binding and cellular uptake assays [37]. Structure-based virtual libraries are screened in silico.…”

Section: Screening Of Combinatorial Librariesmentioning

confidence: 99%

Combinatorial chemistry in drug discovery

Liu

Lam

2017

Current Opinion in Chemical Biology

249

131

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Several combinatorial methods have been developed to create focused or diverse chemical libraries with a wide range of linear or macrocyclic chemical molecules: peptides, non-peptide oligomers, peptidomimetics, small-molecules, and natural product-like organic molecules. Each combinatorial approach has its own unique high-throughput screening and encoding strategy. In this article, we provide a brief overview of combinatorial chemistry in drug discovery with emphasis on recently developed new technologies for design, synthesis, screening and decoding of combinatorial library. Examples of successful application of combinatorial chemistry in hit discovery and lead optimization are given. The limitations and strengths of combinatorial chemistry are also briefly discussed. We are now in a better position to truly leverage the power of combinatorial technologies for the discovery and development of next-generation drugs.

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Advancement and applications of peptide phage display technology in biomedical science

Cited by 286 publications

References 149 publications

Antibody Engineering for Pursuing a Healthier Future

Antibody Engineering for Pursuing a Healthier Future

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Combinatorial chemistry in drug discovery

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