Advancement in analysis of Salviae miltiorrhizae Radix et Rhizoma (Danshen)

Li, Yongguo; Song, Langzhou; Liu, Mei; Zhi-Bi-Hu,; Wang, Zhengtao

doi:10.1016/j.chroma.2008.12.032

Cited by 206 publications

(139 citation statements)

References 73 publications

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“…The obtained results are in agreement with the suggestions that a high concentration of alcohol is preferred for extracting the lipophilic diterpenoids (Zhong et al 2001, Han et al 2008, Li et al 2009). …”

Section: Discussionsupporting

confidence: 82%

“…It is in line with the suggestions that the two types of constituents of Salvia miltiorrhiza, the hydrophilic phenols (e.g. RA) and the lipophilic tanshinones have been proven responsible for the polyvalent activities of this herb (Han et al 2008, Li et al 2009). …”

Section: Discussionsupporting

confidence: 68%

“…danshensu, rosmarinic acid (RA) or salvianolic acid A and B) and lipophilic diterpenoid quinones (e.g. tanshinone I (Tsh 1), tanshinone IIA (Tsh 2A), cryptotanshinone (CTsh)) (Han et al 2008, Li et al 2009). Numerous studies have confirmed a pharmacological activities of S. miltiorrhiza as a cardiovascular protective agent or antioxidant (Matkowski et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Involvement of the different extracts from roots of Salvia miltiorrhiza Bunge on acute hypobaric hypoxia-induced cardiovascular effects in rats – preliminary report

Buchwald¹,

Mikolajczak²,

Krajewska-Patan³

et al. 2012

Polish Journal of Veterinary Sciences

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The present study was carried out to investigate the protective effects of roots of Salvia miltiorrhiza Bunge on hypobaric hypoxia. Two extracts of S. miltiorrhiza (extract 1: ethanol : water -50 : 50; extract 2: 96% ethanol) were used. The experiments were performed after 7 consecutive days of administration of the extracts (200 mg/kg b.w., intragastrically) to male Wistar rats. Next, after placing animals for 60 min in the controlled acute hypobaric hypoxia (500 mm Hg) the systolic arterial blood pressure (SAP) in conscious rats, bioelectric heart activity in unconscious rats and analysis of oxidative stress parameters in the blood of rats: malonyldialdehyde (MDA) and lipid peroxidase (LPO) concentration, activity of superoxide dismutase (SOD) or glutathione peroxidase (GPX) were assayed. It was found out that the extract 1 augmented the lowering of SAP shown in hypoxia affected control rats. On the contrary the extract 2 reversed SAP to values obtained in control animals. Moreover, both extracts led to the normalization of hypoxia-induced tachycardia and levels of MDA, LPO and SOD. It seems that the above-mentioned effects are coupled with different active compounds content in the extracts, however more studies are needed to confirm this hypothesis.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

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Involvement of the different extracts from roots of Salvia miltiorrhiza Bunge on acute hypobaric hypoxia-induced cardiovascular effects in rats – preliminary report

Buchwald¹,

Mikolajczak²,

Krajewska-Patan³

et al. 2012

Polish Journal of Veterinary Sciences

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“…Previous phytochemical studies on RSM indicated that hydrophilic depsides derivatives and lipophilic diterpenoids were the major constituents of this herb [7,8]. Danshensu, protocatechualdehyde, salvianolic acid B, tanshinone I, tanshinone IIA, and cryptotanshinone ( Fig.…”

Section: Introductionmentioning

confidence: 99%

UPLC/Q-TOF-MS Analysis for Identification of Hydrophilic Phenolics and Lipophilic Diterpenoids from Radix Salviae Miltiorrhizae

Yang¹,

Wu²,

Rui³

et al. 2015

Acta Chromatographica

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Summary.A rapid method has been used for simultaneous identification of both hydrophilic and lipophilic compounds from Radix Salviae Miltiorrhizae (RSM, the root of Salvia miltiorrhiza B GE .) by ultra-performance liquid chromatography/quadrupole time-offlight mass spectrometry (UPLC/Q-TOF-MS). A total of 58 compounds extracted by methanol were detected and tentatively identified within 20 min, including hydrophilic phenolics, lipophilic diterpenoids, a verbascose, and several organic acids. These compounds were separated on an Acquity UPLC BEH C18 column and identified based on tandem mass spectrometry (MS/MS) fragmentation patterns under the positive and negative ion modes, respectively. Among them, micranthin B and 9-oxo-10E,12Z-octadecadienoic acid were reported in RSM for the first time. Their fragmentation patterns in electrospray ionization (ESI)-MS/MS spectra were first investigated by matching their accurate molecular masses. This contribution presented one of the first reports on the analysis of hydrophilic phenolics and lipophilic diterpenoids from Radix Salviae Miltiorrhizae using UPLC/Q-TOF-MS. The results demonstrated that UPLC/Q-TOF-MS method could be applied to rapidly and expediently describe and provide comprehensive chemical information for simultaneous analysis of two different polar components in RSM.

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“…The Danshen polyphenols contain one or more ortho-dihydroxy benzene rings, known as catechols, and have one or more carboxylic acid functional groups, except for protocatechuic aldehyde. They are caffeic acid derivatives, occurring as monomers (such as tanshinol and caffeic acid), dimers (such as rosmarinic acid and salvianolic acid D), trimers (such as salvianolic acid A and lithospermic acid), and tetramers (such as salvianolic acid B) (Jiang et al, 2005;Li et al, 2009). Cell-and isolatedtissue-based studies have shown that Danshen catechols exhibited vasodilating, endothelial protective, cardioprotective, antithrombotic, antioxidant, and anti-inflammatory properties; tanshinol and other Danshen catechols also decreased blood pressure in rats and attenuated venular thrombosis, cardiac hypertrophy, and methionine-induced hyperhomocysteinemia in rats (Jiang et al, 2005;Han et al, 2008;Cao et al, 2009;Ho and Hong, 2011;Wang et al, 2013a).…”

Section: Introductionmentioning

confidence: 99%

Systemic Exposure to and Disposition of Catechols Derived from Salvia miltiorrhiza Roots (Danshen) after Intravenous Dosing DanHong Injection in Human Subjects, Rats, and Dogs

Wang

Huang

et al. 2015

Drug Metab Dispos

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DanHong injection is a Danshen (Salvia miltiorrhiza roots)-based injectable solution for treatment of coronary artery disease and ischemic stroke. Danshen catechols are believed to be responsible for the injection's therapeutic effects. This study aimed to characterize systemic exposure to and elimination of Danshen catechols in human subjects, rats, and dogs receiving intravenous DanHong injection. A total of 28 catechols were detected, with content levels of 0.002-7.066 mM in the injection, and the major compounds included tanshinol, protocatechuic aldehyde, salvianolic acid B, rosmarinic acid, salvianolic acids A and D, and lithospermic acid with their daily doses ‡10 mmol/subject. After dosing, tanshinol, salvianolic acid D, and lithospermic acid exhibited considerable exposure in human subjects and rats. However, only tanshinol had considerable exposure in dogs. The considerable exposure to tanshinol was due to its having the highest dose, whereas that to salvianolic acid D and lithospermic acid was due to their relatively long elimination half-lives in the human subjects and rats. Protocatechuic aldehyde and rosmarinic acid circulated in the bloodstream predominantly as metabolites; salvianolic acids A and B exhibited low plasma levels with their human plasma metabolites little or not detected. Tanshinol and salvianolic acid D were eliminated mainly via renal excretion. Elimination of other catechols involved hepatobiliary and/or renal excretion of their metabolites. Methylation was found to be the primary metabolism for most Danshen catechols and showed intercompound and interspecies differences in rate and degree in vitro. The information gained here is relevant to pharmacological and toxicological research on DanHong injection.

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Advancement in analysis of Salviae miltiorrhizae Radix et Rhizoma (Danshen)

Cited by 206 publications

References 73 publications

Involvement of the different extracts from roots of Salvia miltiorrhiza Bunge on acute hypobaric hypoxia-induced cardiovascular effects in rats – preliminary report

Involvement of the different extracts from roots of Salvia miltiorrhiza Bunge on acute hypobaric hypoxia-induced cardiovascular effects in rats – preliminary report

UPLC/Q-TOF-MS Analysis for Identification of Hydrophilic Phenolics and Lipophilic Diterpenoids from Radix Salviae Miltiorrhizae

Systemic Exposure to and Disposition of Catechols Derived from Salvia miltiorrhiza Roots (Danshen) after Intravenous Dosing DanHong Injection in Human Subjects, Rats, and Dogs

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