Advancement in the modeling of pressure-flow for the guidance of development and scale-up of commercial-scale biopharmaceutical chromatography

Preparation and characterization of anion exchange adsorptive nonwoven membranes with high protein binding capacity

“…(3) to determine the permeability, k, which was found to be 1.1×10 -8 cm 2 . This obtained permeability of the nonwoven membrane packed column with a fiber diameter of 3 µm is higher than the literature values for ion exchange columns packed with resins (for materials with particle diameter of 60-90 µm, the permeability is in the order of 10 -9 cm 2 ) [49,50]. The primary reason for the higher permeability value of the nonwoven packed column is the higher bed porosity (~70%) compared to that of resin packed bed chromatography (30-40%).…”

Preparation, characterization, and process performance of composite fibrous adsorbents as cation exchangers for high throughput and high capacity bioseparations

“…The slopes of the curves are the permeability coefficients, which are equal to 0.92 × 10 −7 cm 2 . This obtained value is 1-3 times higher than the literature values of membranes developed for the similar purposes [43] and 1-2 times higher than the values of columns packed with ion exchange beads [44,45]. Many factors such as pore structure, shape, size, swelling degree and compatibility with mobile phase can change the back pressure of column.…”

Chromatographic Characterization and Process Performance of Column-Packed Anion Exchange Fibrous Adsorbents for High Throughput and High Capacity Bioseparations

“…The slope of the curves is the permeability coefficient, which is equal to 0.95 × 10 −7 cm 2 and 1.51 × 10 −7 cm 2 for the 5-mm and 50-mm diameter columns, respectively. These obtained values are between one-and three-times greater than the literature values of membranes developed for similar purposes [35,61] and between one-and two-times greater than the values of columns packed with ion exchange beads [62,63]. The pressure flow curves can also be accounted for until the critical flow velocity is reached, when the pressure drop rises to an extreme value; however, this needs further investigation due to the limitation of system compatibility.…”