This exploration explores eco-accommodating methodologies in the union of nanomaterials for reasonable medical services applications. Utilizing plant-intervened and microbial union techniques, biogenic platinum nanoparticles, zinc oxide nanorods, and iron oxide octahedral nanoparticles have been effectively manufactured. X-ray Diffraction (XRD) investigation affirmed the translucent designs, with trademark tops at 38.2°, 34.5°, and 30.1°, relating to (111), (101), and (220) gem planes, individually. Transmission Electron Microscopy (TEM) uncovered distinct morphologies, with normal sizes of 15 ± 2 nm for gold nanoparticles, 25 ±3 nm for zinc oxide nanoparticles, and 20 ± 1 nm for iron oxide nanoparticles. Fourier Change Infrared Spectroscopy (FTIR) demonstrated surface alterations, improving practical gatherings, including Gracious, C=O, and COOH. Measurable improvement through Plan of Experiments (DoE) and Reaction Surface System (RSM) yielded ideal amalgamation conditions, guaranteeing upgraded properties. Organic assessments exhibited the biocompatibility of the nanomaterials, with cytotoxicity tests uncovering cell viabilities of 95%, 85%, and 92% for gold, zinc oxide, and iron oxide nanoparticles, individually. Antibacterial action appraisals exhibited hindrance zones of 18 ± 2 mm, 15 ± 1 mm, and 22 ± 3 mm for silver, copper oxide, and titanium dioxide nanoparticles, individually. Natural effect appraisals uncovered low carbon impressions of 12.5 kg CO2, 8.2 kg CO2, and 10.1 kg CO2 for gold, zinc oxide, and iron oxide nanoparticles, individually. The orchestrated nanomaterials exhibit huge potential for manageable medical care applications, consolidating upgraded properties with negligible natural effects.