Advancements in culture technology of adipose-derived stromal/stem cells: implications for diabetes and its complications

This study aims to demonstrate the formation of TNTs between AdMSCs and RPE-1 and their alterations in response to experimental stress conditions. Serum starvation was employed as a stress condition to induce TNTs between the AdMSC and RPE-1. The presence of TNTs was demonstrated through immunofluorescence microscopy while scanning electron microscopy was utilized to determine the average thickness. Cell viabilities were assessed after stress by CTG, and H2DCFH-DA probes evaluated the cells' reactive oxygen species (ROS) levels. Further, JC-1 labeled mitochondrial exchange between cells via TNTs was supported by videos. A transmembrane culture system was employed to inhibit TNT formation. In this study, we investigated the role of TNTs in facilitating intercellular communication and mitochondrial transfer between AdMSCs and RPE-1 under stress. We found that TNT-mediated mitochondrial transfer from AdMSCs to RPE-1 helps to reduce ROS levels and improve cell viability. We demonstrated that direct interaction between AdMSCs and RPE-1 was crucial for stress recovery. Co-culture enhanced viability and sustained retinal epithelial cell function after stress-induced damage. Mechanical inhibition of TNT formation decreased cell viability and increased ROS levels, indicating the importance of TNTs in cellular protection. The findings can provide a new perspective on the therapeutic potential of stem cell-based therapy in protecting RPE against stress-induced damage and promoting tissue regeneration.

show abstract

Effects, methods and limits of the cryopreservation on mesenchymal stem cells

Wang,

2024

Stem Cell Res Ther

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are a type of cell capable of regulating the immune system, as well as exhibiting self-renewal and multi-lineage differentiation potential. Mesenchymal stem cells have emerged as an essential source of seed cells for therapeutic cell therapy. It is crucial to cryopreserve MSCs in liquid nitrogen prior to clinical application while preserving their functionality. Furthermore, efficient cryopreservation greatly enhances MSCs’ potential in a range of biological domains. Nevertheless, there are several limits on the MSC cryopreservation methods now in use, necessitating thorough biosafety assessments before utilizing cryopreserved MSCs. Therefore, in order to improve the effectiveness of cryopreserved MSCs in clinical stem cell treatment procedures, new technological techniques must be developed immediately. The study offers an exhaustive analysis of the state-of-the-art MSC cryopreservation techniques, their effects on MSCs, and the difficulties encountered when using cryopreserved MSCs in clinical applications.

show abstract

Advancements in culture technology of adipose-derived stromal/stem cells: implications for diabetes and its complications

Cited by 3 publications

References 207 publications

Advances in nucleic acid delivery strategies for diabetic wound therapy

Advances in nucleic acid delivery strategies for diabetic wound therapy

Adipose-derived Mesenchymal Stem Cells and Retinal Pigment Epithelial Cells Interactions in Stress Environment via Tunneling Nanotubes

Effects, methods and limits of the cryopreservation on mesenchymal stem cells

Contact Info

Product

Resources

About