Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices

Cappai, Francesco; Garcia, Alexandria; Cullen, Ryan; Davis, Matthew; Muńoz, Patricio

doi:10.3390/plants9111624

Cited by 11 publications

(24 citation statements)

References 52 publications

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“…The results of this study showed that when using 1.5% NaClO for 8 min, and then 0.1% HgCl 2 for 2 min for explant surface sterilization, the survival rate (viability) was higher than 85 % ('Biloxi': 86.67 %; 'Bluecrop': 93.33 %). However, our results agree with Cappai et al [29], pointing out that it depends on the genotype introduced in the in vitro culture conditions, in fact, modifications of the exposure (in time and concentration) to the disinfectant compound(s) can lead to significant variations in the results.…”

Section: Micropropagation Phasesupporting

confidence: 91%

Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization

Tejada-Alvarado

Meléndez-Mori

Valqui

et al. 2022

JBR

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BACKGROUND: Blueberry production has generated great commercial expectations, therefore for its agricultural expansion it is necessary to overcome the challenges at the time of mass propagation. OBJECTIVE: Evaluate the effect of a set of factors influencing micropropagation, as well as the influence of substrates on the ex vitro morpho-physiological performance of blueberry seedlings. METHODS: A set of protocols were developed to optimize all stages of micropropagation (aseptic establishment, multiplication, rooting, and acclimatization) of blueberries. RESULTS: Explants immersed in 1.5% NaClO for 8 min and then in 0.1% HgCl2 for 2 min achieved 100% sterility and a viability rate of 86.67% for ‘Biloxi’ and 93.33% for ‘Bluecrop’. At the multiplication stage, the maximum number of shoots of ‘Biloxi’ (3.53) and ‘Bluecrop’ (2.27) were obtained on the medium supplemented with 0.2 and 10 mg L–1 silver nanoparticles (AgNPs), respectively. The percentage of in vitro rooting was significantly improved on media containing activated charcoal, with levels between 80% and 100% . In the acclimatization phase, plants grown in a substrate composed of peat and cocomix® (2:1 ratio) showed greater uniformity and better morpho-physiological behavior. CONCLUSIONS: The present results could be successfully used for large-scale commercial production of blueberries of the varieties ‘Biloxi’ and ‘Bluecrop’.

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Section: Micropropagation Phasesupporting

confidence: 91%

Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization

Tejada-Alvarado

Meléndez-Mori

Valqui

et al. 2022

JBR

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“…However, to our knowledge, there is only one study of CRISPR-Cas9 targeted mutagenesis in blueberry (Omori et al, 2021 ). At the UF blueberry breeding program, we have advanced our understanding of the best tissue culture practices and most effective transformation markers (Cappai et al, 2020b ), laying the ground for CRISPR/Cas9 genome editing implementation in our breeding program. Using this technique, we can also take advantage of the knowledge accumulated from model crops to introduce novel allelic diversity in orthologs and accelerate the domestication process.…”

Section: Resultsmentioning

confidence: 99%

Genomic Selection in an Outcrossing Autotetraploid Fruit Crop: Lessons From Blueberry Breeding

et al. 2021

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Blueberry (Vaccinium corymbosum and hybrids) is a specialty crop with expanding production and consumption worldwide. The blueberry breeding program at the University of Florida (UF) has greatly contributed to expanding production areas by developing low-chilling cultivars better adapted to subtropical and Mediterranean climates of the globe. The breeding program has historically focused on recurrent phenotypic selection. As an autopolyploid, outcrossing, perennial, long juvenile phase crop, blueberry breeding cycles are costly and time consuming, which results in low genetic gains per unit of time. Motivated by applying molecular markers for a more accurate selection in the early stages of breeding, we performed pioneering genomic selection studies and optimization for its implementation in the blueberry breeding program. We have also addressed some complexities of sequence-based genotyping and model parametrization for an autopolyploid crop, providing empirical contributions that can be extended to other polyploid species. We herein revisited some of our previous genomic selection studies and showed for the first time its application in an independent validation set. In this paper, our contribution is three-fold: (i) summarize previous results on the relevance of model parametrizations, such as diploid or polyploid methods, and inclusion of dominance effects; (ii) assess the importance of sequence depth of coverage and genotype dosage calling steps; (iii) demonstrate the real impact of genomic selection on leveraging breeding decisions by using an independent validation set. Altogether, we propose a strategy for using genomic selection in blueberry, with the potential to be applied to other polyploid species of a similar background.

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“…To our knowledge, there is only one study of CRISPR-Cas9 targeted mutagenesis in blueberry (Omori et al , 2021). At the UF blueberry breeding program, we have advanced our understanding on the best tissue culture practices and most effective transformation markers (Cappai et al , 2020b), laying the ground for CRISPR/Cas9 genome editing implementation in our breeding program. Using this technique, we can also take advantage of the knowledge accumulated from model crops to introduce novel allelic diversity in orthologues and accelerate the domestication process.…”

Section: Resultsmentioning

confidence: 99%

Genomic prediction in an outcrossing and autotetraploid fruit crop: lessons from blueberry breeding

Ferrão

Amadeu

Benevenuto

et al. 2021

Preprint

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Blueberry (Vaccinium corymbosum and hybrids) is a specialty crop, with expanding production and consumption worldwide. The blueberry breeding program at the University of Florida (UF) has greatly contributed to the expansion of production areas by developing low-chilling cultivars better adapted to subtropical and Mediterranean climates of the globe. The breeding program has historically focused on phenotypic recurrent selection. As an autopolyploid, outcrossing, perennial, long juvenile phase crop, blueberrys breeding cycles are costly and time-consuming, which results in low genetic gains per unit of time. Motivated by the application of molecular markers for a more accurate selection in early stages of breeding, we performed pioneering genomic prediction studies and optimization for implementation in the blueberry breeding program. We have also addressed some complexities of sequence-based genotyping and model parametrization for an autopolyploid crop, providing empirical contributions that can be extended to other polyploid species. We herein revisited some of our previous genomic prediction studies and described the current achievements in the crop. In this paper, our contribution for genomic prediction in an autotetraploid crop is three-fold: i) summarize previous results on the relevance of model parametrizations, such as diploid or polyploid methods, and inclusion of dominance effects; ii) assess the importance of sequence depth of coverage and genotype dosage calling steps; iii) demonstrate the real impact of genomic selection on leveraging breeding decisions by using an independent validation set. Altogether, we propose a strategy for the use of genomic selection in blueberry, with potential to be applied to other polyploid species of a similar background.

show abstract

Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices

Cited by 11 publications

References 52 publications

Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization

Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization

Genomic Selection in an Outcrossing Autotetraploid Fruit Crop: Lessons From Blueberry Breeding

Genomic prediction in an outcrossing and autotetraploid fruit crop: lessons from blueberry breeding

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