Advances and applications of nanophotonic biosensors

Altug, Hatice; Oh, Sang‐Hyun; Maier, Stefan A.; Homola, Jiřı́

doi:10.1038/s41565-021-01045-5

Cited by 496 publications

(286 citation statements)

References 155 publications

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“…Because prion proteins have strong interactions with simply functionalized metallic surfaces, besides AuNPs, we envision a broad range of metallic nanoparticles with various materials and shapes to be useful in detection. Additionally, substrate-based nanostructures exhibiting optical resonances could be useful in detecting conformational changes via other sensing modalities such as surface-enhanced infrared absorption spectroscopy 60,61 to further improve the speed and accuracy of prion detection. Examining other areas of the electromagnetic spectrum, such as tera-Hertz sensing, could also lead to improved detection.…”

Section: Discussionmentioning

confidence: 99%

A Field-Deployable Diagnostic Assay for the Visual Detection of Misfolded Prions

Christenson

Rowden

et al. 2021

Preprint

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Diagnostic tools for the detection of protein-misfolding diseases (i.e., proteopathies) are limited. Gold nanoparticles (AuNPs) facilitate sensitive diagnostic techniques via visual color change for the detection of a variety of targets. In parallel, recently developed quaking-induced conversion (QuIC) assays leverage protein-amplification and fluorescent signaling for the accurate detection of misfolded proteins. Here, we combine AuNP and QuIC technologies for the visual detection of amplified misfolded prion proteins from tissues of wild white-tailed deer infected with chronic wasting disease (CWD), a prion disease of cervids. Our newly developed assay, MN-QuIC™, enables both naked-eye and light-absorbance measurements for detection of misfolded prions. MN-QuIC™ leverages basic laboratory equipment that is cost-effective and portable, thus facilitating real-time prion diagnostics across a variety of settings. To test the portability of our assay, we deployed to a rural field-station in southeastern Minnesota and tested for CWD on site. We successfully demonstrated that MN-QuIC™ is functional in a non-traditional laboratory setting by performing a blinded analysis in the field and correctly identifying all CWD positive and CWD not detected deer at the field site in less than 24 hours, thus documenting the portability of the assay. Additionally, we show that electrostatic forces help govern the AuNP/prion interactions. Importantly, all of our white-tailed deer (n=37) were independently tested using ELISA, IHC, and/or RT-QuIC technologies and results secured with MN-QuIC™ were 100% consistent with these tests. We conclude that hybrid AuNP and QuIC assays, such as MN-QuIC™, have great potential for sensitive, field-deployable diagnostics of a variety of protein misfolding diseases.Statement of SignificancePortable diagnostic tools for the detection of prion and protein-misfolding diseases are limited. Using chronic wasting disease (CWD) as a model, we demonstrate the functionality of a gold nanoparticle (AuNP)-based assay for the field-based detection of prion diseases. CWD is spreading through cervid populations across North America and the disease poses significant threats to a number of economic sectors, with global implications. Our newly developed diagnostic assay leverages the visible color change of AuNPs to detect the presence of CWD-causing prions. The assay can be performed using lowcost equipment and reagents, thus having great potential to expand prion disease surveillance efforts and prevent infectious prions from entering the human food chain.

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Section: Discussionmentioning

confidence: 99%

A Field-Deployable Diagnostic Assay for the Visual Detection of Misfolded Prions

Christenson

Rowden

et al. 2021

Preprint

Self Cite

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“…The same biointerface was then used for the analysis of antiHBs in saliva samples, where, due to the lower concentration compared to blood plasma, the analysis had to be performed with the sandwich assay format and PEF readout [27]. Let us note that a thorough review of these developments has been conducted by more specialized publications [28][29][30]. [22] with permission from Elsevier.…”

Section: Chemosensor Types 21 State-of-the-art Of Plasmonic Sensorsmentioning

confidence: 99%

State of the Art of Chemosensors in a Biomedical Context

et al. 2022

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Healthcare is undergoing large transformations, and it is imperative to leverage new technologies to support the advent of personalized medicine and disease prevention. It is now well accepted that the levels of certain biological molecules found in blood and other bodily fluids, as well as in exhaled breath, are an indication of the onset of many human diseases and reflect the health status of the person. Blood, urine, sweat, or saliva biomarkers can therefore serve in early diagnosis of diseases such as cancer, but also in monitoring disease progression, detecting metabolic disfunctions, and predicting response to a given therapy. For most point-of-care sensors, the requirement that patients themselves can use and apply them is crucial not only regarding the diagnostic part, but also at the sample collection level. This has stimulated the development of such diagnostic approaches for the non-invasive analysis of disease-relevant analytes. Considering these timely efforts, this review article focuses on novel, sensitive, and selective sensing systems for the detection of different endogenous target biomarkers in bodily fluids as well as in exhaled breath, which are associated with human diseases.

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“…Coupling of the propagating electromagnetic waves into resonant oscillations of free electron plasma (surface plasmons (SPs)) propagating at the interface of the noble metal and dielectric is widely used to enhance light–matter interaction in novel optoelectronic devices, metasurfaces and chemo- and biosensors [ 1 , 2 , 3 , 4 , 5 , 6 ]. SPs can be localized within nanoscale noble metal structures acting as the resonators, while their geometry defines the spectral position and Q-factor of the SP resonance.…”

Section: Introductionmentioning

confidence: 99%

Tuning Collective Plasmon Resonances of Femtosecond Laser-Printed Metasurface

et al. 2022

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The optical response of properly excited periodically arranged plasmonic nanostructures is known to demonstrate sharp resonance features associated with high-Q collective modes demanding for various applications in light–matter interaction, filtering and sensing. Meanwhile, practical realization and replication of plasmonic platforms supporting high-Q modes via scalable inexpensive lithography-free approach is still challenging. Here, we justify direct ablation-free irradiation of Si-supported thin Au film by nanojoule-energy femtosecond laser pulses as a single-step and scalable technology for realization of plasmonic metasurfaces supporting collective plasmonic response. Using an adjustable aperture to control and upscale the size of the fabricated nanostructures, nanobumps and nanojets, we demonstrated plasmonic metasurface supporting collective resonances with a moderately high Q-factor (up to 17) and amplitude (up to 45%) within expanded spectral range (1.4–4.5 µm). Vacuum deposition of thin films above the as-fabricated nanostructure arrays was demonstrated to provide fine tuning of the resonance position, also expanding the choice of available materials for realization of plasmonic designs with extended functionality.

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Advances and applications of nanophotonic biosensors

Cited by 496 publications

References 155 publications

A Field-Deployable Diagnostic Assay for the Visual Detection of Misfolded Prions

A Field-Deployable Diagnostic Assay for the Visual Detection of Misfolded Prions

State of the Art of Chemosensors in a Biomedical Context

Tuning Collective Plasmon Resonances of Femtosecond Laser-Printed Metasurface

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