Advances and challenges for fluorescence nanothermometry

Zhou, Jiajia; Rosal, Blanca del; Jaque, Daniel; Uchiyama, Seiichi; Jin, Dayong

doi:10.1038/s41592-020-0957-y

Cited by 451 publications

(384 citation statements)

References 105 publications

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“…In the presentation here, nanosensors were allowed to find the temperature and pH by using two fluorescent ratios. It should be noted that such an approach was reported for a silica-based pH and oxygen sensor [14]. Both fluorescent ratios reported in that work were independent of each other.…”

Section: Introductionmentioning

confidence: 64%

Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements

2021

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The mesoporous nature of silica nanoparticles provides a novel platform for the development of ultrabright fluorescent particles, which have organic molecular fluorescent dyes physically encapsulated inside the silica pores. The close proximity of the dye molecules, which is possible without fluorescence quenching, gives an advantage of building sensors using FRET coupling between the encapsulated dye molecules. Here we present the use of this approach to demonstrate the assembly of ultrabright fluorescent ratiometric sensors capable of simultaneous acidity (pH) and temperature measurements. FRET pairs of the temperature-responsive, pH-sensitive and reference dyes are physically encapsulated inside the silica matrix of ~50 nm particles. We demonstrate that the particles can be used to measure both the temperature in the biologically relevant range (20 to 50 °C) and pH within 4 to 7 range with the error (mean absolute deviation) of 0.54 °C and 0.09, respectively. Stability of the sensor is demonstrated. The sensitivity of the sensor ranges within 0.2–3% °C−1 for the measurements of temperature and 2–6% pH−1 for acidity.

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Section: Introductionmentioning

confidence: 64%

Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements

2021

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“…various FRET biosensors. Temperature control is essential for cell viability, and growing evidence highlights the importance of the temperature gradients that are experienced by metabolically active biological tissues (Chrétien et al, 2018;Ogle et al, 2020;Zhou et al, 2020). Importantly, in breast and colon cancer cells, temperature gradients have been linked to the function of the uncoupling protein UCP1, mitochondrial function and cell oxygenation (Jenkins et al, 2016;Kawashima et al, 2020).…”

Section: Protein Interactionsmentioning

confidence: 99%

Luminescence lifetime imaging of three-dimensional biological objects

Dmitriev

Intes

Barroso

2021

Journal of Cell Science

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A major focus of current biological studies is to fill the knowledge gaps between cell, tissue and organism scales. To this end, a wide array of contemporary optical analytical tools enable multiparameter quantitative imaging of live and fixed cells, three-dimensional (3D) systems, tissues, organs and organisms in the context of their complex spatiotemporal biological and molecular features. In particular, the modalities of luminescence lifetime imaging, comprising fluorescence lifetime imaging (FLI) and phosphorescence lifetime imaging microscopy (PLIM), in synergy with Förster resonance energy transfer (FRET) assays, provide a wealth of information. On the application side, the luminescence lifetime of endogenous molecules inside cells and tissues, overexpressed fluorescent protein fusion biosensor constructs or probes delivered externally provide molecular insights at multiple scales into protein–protein interaction networks, cellular metabolism, dynamics of molecular oxygen and hypoxia, physiologically important ions, and other physical and physiological parameters. Luminescence lifetime imaging offers a unique window into the physiological and structural environment of cells and tissues, enabling a new level of functional and molecular analysis in addition to providing 3D spatially resolved and longitudinal measurements that can range from microscopic to macroscopic scale. We provide an overview of luminescence lifetime imaging and summarize key biological applications from cells and tissues to organisms.

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“…With these nanoprobes, typical luminescence-based nanothermometry consists of a ratiometric approach, consisting in measuring the luminescence intensity ratio (LIR) of two emission peaks that are in thermal equilibrium. To compare the thermal performances of different types of nanoprobes, a relative thermal sensitivity, Sr, has been defined as [16]:…”

Section: Introductionmentioning

confidence: 99%

Luminescent Yb3+,Er3+-Doped α-La(IO3)3 Nanocrystals for Neuronal Network Bio-Imaging and Nanothermometry

2021

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Dual-light emitting Yb3+,Er3+-codoped α-La(IO3)3 nanocrystals, known to exhibit both second harmonic signal and photoluminescence (PL), are evaluated as optical nanoprobes and thermal sensors using both conventional microscopes and a more sophisticated micro-PL setup. When loaded in cortical and hippocampal neurons for a few hours at a concentration of 0.01 mg/mL, a visible PL signal arising from the nanocrystals can be clearly detected using an epifluorescent conventional microscope, enabling to localize the nanocrystals along the stained neurons and to record PL variation with temperature of 0.5% K−1. No signal of cytotoxicity, associated with the presence of nanocrystals, is observed during the few hours of the experiment. Alternatively, a micro-PL setup can be used to discriminate the different PL lines. From ratiometric PL measurements, a relative thermal sensitivity of 1.2% K−1 was measured.

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Advances and challenges for fluorescence nanothermometry

Cited by 451 publications

References 105 publications

Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements

Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements

Luminescence lifetime imaging of three-dimensional biological objects

Luminescent Yb3+,Er3+-Doped α-La(IO3)3 Nanocrystals for Neuronal Network Bio-Imaging and Nanothermometry

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