Advances and challenges in liposome digestion: Surface interaction, biological fate, and GIT modeling

Li, Weilin; Ye, Aiqian; Han, Feifei; Han, Jianzhong

doi:10.1016/j.cis.2018.11.007

Cited by 133 publications

(62 citation statements)

References 163 publications

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“…Prema dostupnim literaturnim podacima, do sada su se lipozomi ograničeno ispitivali za inkapsulaciju hidrolizata proteina surutke [16], kazeina [13,17,18], pasulja [19] i ribljih proteina [20], ali podaci o inkapsulaciji hidrolizata proteina soje u lipozome izostaju. Stoga, cilj ovog rada bio je ispitivanje mogućnosti upotrebe lipozoma kao nosača za inkapsulaciju proteinskih hidrolizata soje, ispitivanje uticaja procesa inkapsulacije na antioksidativnu aktivnost hidrolizata proteina soje, ali i praćenje oslobađanja inkapsuliranih peptida u simuliranim gastrointestinalnim uslovima.…”

Section: A R T I C L E I N P R E S Sunclassified

“…U poređenju sa liposferama, lipozomi su uspešniji sistemi za inkapsulaciju proteina zbog svojih višestrukih prednosti: biorazgradljivosti, niske toksičnosti i slabe imunogenosti, poboljšanje rastvorljivosti bioaktivne komponente, i mogućnosti površinskih modifikacija zarad ciljanog, produženog i neprekidnog otpuštanja proteina [11,12]. Svaka lipozomna koloidna čestica sadrži polarne, nepolarne i amfifilne delove, čineći lipozome na taj način biokompatibilnim, kako sa hidrofobnim, tako i sa hidrofilnim bioaktivnim peptidima [13,14]. Primarni izazov sa kojim se suočava postupak implementacije bioaktivnih peptida u komercijalne prehrambene proizvode jeste osteljivost peptida u gastrointestinalnom traktu na dejstvo digestivnih enzima, pepsina u želudcu i alkalne proteaze u pankreasu, čijim delovanjem dolazi do smanjenja dužine i promene sekvence peptida, i gubitka njihove biološke funkcije [15].…”

Section: Introductionunclassified

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Production and characterization of liposomes with encapsulated bioactive soy protein hydrolysate

Pavlović¹,

Jovanović

Đorđević

et al. 2020

Hem Ind

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Soy proteins known for their high nutritional value and pronounced techno-functional properties, can be hydrolyzed by using proteolytic enzymes and thus converted into hydrolysates rich in di-, tri- and oligopeptides. The resulting peptides are carriers of valuable biological activities, which make the soy hydrolysates very important in functional food applications as techno-functional and bioactive ingredients. However, commercial incorporation and application of soy protein hydrolysates can be hinderedby their low bioavailability and instability, bitter taste, hygroscopicity and possibility to interact with the food matrix. The aim of this research is encapsulation of the soy protein hydrolysate in liposomes in order to overcome the stated shortcomings, while preserving the biological activities that protein hydrolysates exhibit. The soy hydrolysate was prepared by a two-step enzymatic hydrolysis of a soy protein concentrate using commercial food-grade proteases, endoprotease from Bacillus amyloliquefaciens (Neutrase?) and egzo- and endoprotease from Aspergillus oryzae (Flavourzyme?) and encapsulated within liposomes. The liposomes were produced by a thin film method using a commercial lipid mixture (Phospolipon? 90G) containing mainly phosphatidylcholine. Next, the obtained multilamellar vesicles (MLV) with the soy protein hydrolysate were treated by high-intensity ultrasound waves generated by using (1) an ultrasonic probe at a frequency of 20 kHz and (2) an ultrasonic bath with a frequency 40 kHz. The smallest (310 nm) and uniform (unimodal size distribution) liposomes with the highest efficiency of peptide encapsulation (19 %) were obtained by the probe sonication. The presented results showed that incorporation of the soy protein hydrolysates was achieved within the liposome membrane and caused an increase in the liposome size in all tested formulations, namely: from 297 to 310 nm by using the ultrasonic probe, from 722 to 850 nm by using the ultrasonic bath, while in formulations without the ultrasonic treatments the increase from 2818 to 3464 nm was recorded. The entrapped peptides caused enlargement of all liposomes and the increase in negative charge of zeta potential values, which in the case of MLV liposomes was below -30 mV, indicating high stability of these liposomes. Significant antioxidant activity of the probe-sonicated liposomal formulation was confirmed by the ABTS scavenging ability and iron-chelating activity. Release studies conducted under simulated gastrointestinal conditions confirmed that liposomes provide prolonged release of encapsulated soy protein hydrolysates as compared to diffusion of the free hydrolysate. In the first 75 min, only 20 % of liposome encapsulated soy peptides diffused, which is 2.2-fold lower as compared to the diffusion of the non-encapsulated soy hydrolysate. Liposome encapsulated soy protein hydrolysates may provide the possibility for application in the areas such as food science and technology, with the aim to enhance the nutritional value and shelf life of food products, and develop functional foods.

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Section: A R T I C L E I N P R E S Sunclassified

Section: Introductionunclassified

Production and characterization of liposomes with encapsulated bioactive soy protein hydrolysate

Pavlović¹,

Jovanović

Đorđević

et al. 2020

Hem Ind

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“…SGF and SIF are typically prepared based on the United States Pharmacopeia recipe with some modifications. The ingredients of in vitro digestion fluids have been reviewed previously (Cook et al., ; Liu, Ye, Han, & Han, ; Marzorati & Van de Wiele, ). SGF (pH 1 to 2) consists of HCl, salts, and pepsin, while SIF (pH 5 to 7) consists of bile salts and pancreatin (Krasaekoopt, Bhandari, & Deeth, ; Lee & Heo, ; Sun & Griffiths, ).…”

Section: Emerging Methods For Evaluating Probiotic Delivery System Efmentioning

confidence: 99%

“…For instance, they have been used to study the germination and survival of probiotics (Keller, Verbruggen, Cash, Farmer, & Venema, ; Surono et al., ). Figure 5B shows an example of a multicompartment model of TIM‐1 (Liu et al., ). More recently, an in vitro gastrointestinal model called “The Smallest Intestine” (TSI) model has been developed to simulate the human small intestine, which maintains the pH, temperature, bile salt levels, microbiota, and enzymes composition at physiologically relevant levels (Cieplak et al., ).…”

Section: Emerging Methods For Evaluating Probiotic Delivery System Efmentioning

confidence: 99%

Progress in microencapsulation of probiotics: A review

Yao

Xie

et al. 2020

Comp Rev Food Sci Food Safe

328

160

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The potential health benefits of probiotics may not be realized because of the substantial reduction in their viability during food storage and gastrointestinal transit. Microencapsulation can be used to enhance the resistance of probiotics to unfavorable conditions. A range of oral delivery systems has been developed to increase the level of probiotics reaching the colon including embedding and coating systems. This review introduces emerging strategies for the microencapsulation of probiotics and highlights the key mechanisms of their stress–tolerance properties. Recent in vitro and in vivo models for evaluation of the efficiency of probiotic delivery systems are also reviewed. Encapsulation technologies are required to maintain the viability of probiotics during storage and within the human gut so as to increase their ability to colonize the colon. These technologies work by protecting the probiotics from harsh environmental conditions, as well as increasing their mucoadhesive properties. Typically, the probiotics are either embedded inside or coated with food‐grade materials such as biopolymers or lipids. In some cases, additional components may be coencapsulated to enhance their viability such as nutrients or protective agents. The importance of having suitable in vitro and in vivo models to evaluate the efficiency of probiotic delivery systems is also emphasized.

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“…The liposomes, vesicular systems based on lipids, in the last decades have increased their potential of their applications in various scientific disciplines and extensively been used as carriers for numerous molecules in cosmetic and pharmaceutical industries and medical and agricultural fields due to their high capacity and efficiency in drug delivery [1,2]. For purposes in drug delivery systems, the liposomes are suitable candidates, due to their multiple properties that make them unique in these processes, such as biocompatibility, biodegradability, low toxicity, ability to modify the pharmacokinetic profile of the drug loaded, etc.…”

Section: Introductionmentioning

confidence: 99%

Dissipative Particle Dynamics Simulations of Self-Assemblies of Liposomes for Drug Delivery Applications

Terrón-Mejía¹,

Higuera‐Ciapara²,

Martínez-Benavidez³

et al. 2019

Liposomes - Advances and Perspectives

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Liposomes are essential components in the development of functional materials for drug delivery; this is mainly due to its ability to self-associate spontaneously and form bilayer vesicles. In these potential applications, knowing the size of selfassembled liposomes is essential for optimal performance; however, this process still has many unanswered questions. Conventional experimental techniques to study self-assemblies of liposome nanoparticles still have a great challenge. Computational simulations emerge as an alternative to understand the role of thermodynamic properties responsible for the self-assembly, particularly when they are unreachable experimentally because of limited time and length resolutions. In this chapter, we present the advantages and disadvantages of dissipative particle dynamic method to explore the functioning of liposome self-assembly in the transport of drugs.

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Advances and challenges in liposome digestion: Surface interaction, biological fate, and GIT modeling

Cited by 133 publications

References 163 publications

Production and characterization of liposomes with encapsulated bioactive soy protein hydrolysate

Production and characterization of liposomes with encapsulated bioactive soy protein hydrolysate

Progress in microencapsulation of probiotics: A review

Dissipative Particle Dynamics Simulations of Self-Assemblies of Liposomes for Drug Delivery Applications

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