Advances and challenges in medicinal plant breeding

Wang, Wenle; Xu, Jinfan; Fang, Hao; Li, Zhijun; Li, Min-Hui

doi:10.1016/j.plantsci.2020.110573

Cited by 85 publications

(37 citation statements)

References 23 publications

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“…The use of medicinal plants as the first line approach is a practice recognized by 80% of the world population for the primary health care [52][53][54]. In this study, we observed a high rate (73,6%) of malaria self-medication with medicinal plants suggesting that the use of medicinal plants in Lubumbashi is a popular knowledge.…”

Section: Discussionmentioning

confidence: 54%

Self-medication with antimalarials drugs in Lubumbashi city (DR Congo)

Valentin

Salvius

Philippe

et al. 2020

GSC Biol. and Pharm. Sci.

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Self-medication for malaria is very common in Sub-Saharan Africa where this parasitosis is endemic. In order to determine the extent, characteristics and factors associated with this practice in medical area in the city of Lubumbashi, a cross-sectional descriptive study was carried out by direct interview from January 2018 to June 2018, in the internal medicine departments of 10 General Referral Hospital (GRH) . Five thousand one hundred and thirtyfour patients were consulted among them, 96.6% (average age 38.7 ± 8 years; average income: 100 ± 12 USD; gender male/ female ratio: 0.86) practice self-medication with antimalarials. They used it for the first time at an average age of 13-17 years. Quinine (36.4%) and Carica papaya (0.4%) are the most used remedies in conventional and non-conventional medicine, respectively. Several risks are incurred during this practice when the most cited are worsening side effects (53%), incomplete treatment (37%) and appearance to health sciences (37%). Age (17-35 and > 65 years old), low income (50-150 USD), membership in a health sector and the claim to know antimalarial drugs, predispose patients to self-medication. There is an urgent need to regulate this practice to avoid its harmful consequences.

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Section: Discussionmentioning

confidence: 54%

Self-medication with antimalarials drugs in Lubumbashi city (DR Congo)

Valentin

Salvius

Philippe

et al. 2020

GSC Biol. and Pharm. Sci.

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“…High heterozygosity is one of many recognised challenges facing plant breeding of medicinal plants ( Wang et al, 2020 ), and one expected for S. flavescens based on anecdotal evidence that it was an outbreeding species. However, in attempting to make crosses, we noted that anthesis can occur before flowers open ( Figure 3 ), raising the possibility that the species might be self-pollinated.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Genetic Diversity in the Traditional Chinese Medicine Plant ‘Kushen’ (Sophora flavescens Ait.)

et al. 2021

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Kushen root, from the woody legume Sophora flavescens, is a traditional Chinese medicine that is a key ingredient in several promising cancer treatments. This activity is attributed in part to two quinolizidine alkaloids (QAs), oxymatrine and matrine, that have a variety of therapeutic activities in vitro. Genetic selection is needed to adapt S. flavescens for cultivation and to improve productivity and product quality. Genetic diversity of S. flavescens was investigated using genotyping-by-sequencing (GBS) on 85 plants grown from seeds collected from 9 provinces of China. DArTSeq provided over 10,000 single nucleotide polymorphism (SNP) markers, 1636 of which were used in phylogenetic analysis to reveal clear regional differences for S. flavescens. One accession from each region was selected for PCR-sequencing to identify gene-specific SNPs in the first two QA pathway genes, lysine decarboxylase (LDC) and copper amine oxidase (CAO). To obtain SfCAO sequence for primer design we used a targeted transcript capture and assembly strategy using publicly available RNA sequencing data. Partial gene sequence analysis of SfCAO revealed two recently duplicated genes, SfCAO1 and SfCAO2, in contrast to the single gene found in the QA-producing legume Lupinus angustifolius. We demonstrate high efficiency converting SNPs to Kompetitive Allele Specific PCR (KASP) markers developing 27 new KASP markers, 17 from DArTSeq data, 7 for SfLDC, and 3 for SfCAO1. To complement this genetic diversity analysis a field trial site has been established in South Australia, providing access to diverse S. flavescens material for morphological, transcriptomic, and QA metabolite analysis. Analysis of dissected flower buds revealed that anthesis occurs before buds fully open suggesting a potential for S. flavescens to be an inbreeding species, however this is not supported by the relatively high level of heterozygosity observed. Two plants from the field trial site were analysed by quantitative real-time PCR and levels of matrine and oxymatrine were assessed in a variety of tissues. We are now in a strong position to select diverse plants for crosses to accelerate the process of genetic selection needed to adapt kushen to cultivation and improve productivity and product quality.

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“…Chen. (Wang et al, 2020). Biotechnology techniques play an important role in the conservation of some medicinal plants -in particular endangered species.…”

Section: Modern Approaches For Breeding Of Medicinal Plantsmentioning

confidence: 99%

Breeding of medicinal and essential oil crops in VILAR: achievements and prospects

et al. 2021

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This review discusses the main methods of breeding material development, the current state, problems and prospects for medicinal and essential oil plants breeding. The relevance of this area has especially increased due to the sanctions, the resulting shortage of medicinal plants and their low quality, which does not meet the requirements of the pharmaceutical industry. To produce a stable plant raw material base, it is necessary to actively develop a breeding process to create new highly productive varieties of medicinal plants resistant to biotic and abiotic environments. In breeding with the use of modern molecular biological methods, related species and generic complexes of the All-Russian Research Institute of Medicinal and Aromatic Plants (VILAR) collection can be involved, where there is extensive original genetic material of medicinal, essential oil, rare and endangered species. In the breeding of medicinal and essential oil crops, traditional methods of individual and individual-family selection, polyploidy, chemical mutagenesis and a combination of methods to obtain original breeding material are still promising. VILAR has created more than 90 varieties of medicinal and essential oil crops, most of which have been approved for use throughout the Russian Federation.

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Advances and challenges in medicinal plant breeding

Cited by 85 publications

References 23 publications

Self-medication with antimalarials drugs in Lubumbashi city (DR Congo)

Self-medication with antimalarials drugs in Lubumbashi city (DR Congo)

Analysis of Genetic Diversity in the Traditional Chinese Medicine Plant ‘Kushen’ (Sophora flavescens Ait.)

Breeding of medicinal and essential oil crops in VILAR: achievements and prospects

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