Advances in algal lipid metabolism and their use to improve oil content

Kong, Fantao; Blot, Carla; Liu, Keqing; Kim, Minjae; Li-Beisson, Yonghua

doi:10.1016/j.copbio.2024.103130

Current Opinion in Biotechnology

2024

DOI: 10.1016/j.copbio.2024.103130

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Advances in algal lipid metabolism and their use to improve oil content

Fantao Kong,

Carla Blot,

Keqing Liu

et al.

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2024

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Cited by 3 publications

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Photoproduction of Aviation Fuel β‐Caryophyllene From the Eukaryotic Green Microalga Chlamydomonas reinhardtii

Dou,

Li,

et al. 2024

Biotech & Bioengineering

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ABSTRACTβ‐caryophyllene is a plant‐derived sesquiterpene and is regarded as a promising ingredient for aviation fuels. Microalgae can convert CO2 into energy‐rich bioproducts through photosynthesis, making them potential platforms for the sustainable production of sesquiterpenes. However, heterologous sesquiterpene engineering in microalgae is still in its infancy, and β‐caryophyllene production in eukaryotic photosynthetic microorganisms has not been reported. In this study, we succeeded in producing β‐caryophyllene in the model eukaryotic microalga Chlamydomonas reinhardtii by heterologously expressing a β‐caryophyllene synthase (QHS). Furthermore, overexpressing the key enzyme of the 2‐C‐methyl‐D‐erythritol 4‐phosphate pathway in the QHS‐expressing strain (QHS‐DXS‐HDR−18) resulted in a 17‐fold higher β‐caryophyllene production compared to the single expression of QHS (QHS−28). Additionally, when isopentenyl diphosphate isomerase (CrIDI) was overexpressed, the β‐caryophyllene production was up to 480.6 μg/L in QHS‐DXS‐HDR‐CrIDI−16 and increased by 1.8‐fold compared to the parental strain QHS‐DXS‐HDR−18. Under photoautotrophic and photomixotrophic conditions in photobioreactors, the β‐caryophyllene production in QHS‐DXS‐HDR‐CrIDI−16 reached 854.7 and 1016.8 μg/L, respectively. Noticeably, all the β‐caryophyllene‐producing strains generated in this study did not exhibit adverse effects on cell growth and photosynthesis activity compared to the untransformed strain. This study demonstrates the first successful attempt to produce β‐caryophyllene in the eukaryotic microalga C. reinhardtii and develops a novel strategy for increasing sesquiterpene production in eukaryotic photosynthetic microorganisms.

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Photoproduction of Aviation Fuel β‐Caryophyllene From the Eukaryotic Green Microalga Chlamydomonas reinhardtii

Dou,

Li,

et al. 2024

Biotech & Bioengineering

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Editorial overview: Plant synthetic biology

Hanson,

Voiniciuc

2024

Current Opinion in Biotechnology

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Microalgae Biotechnology: Methods and Applications

Wang,

Ma,

Kong

2024

Bioengineering

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Advances in algal lipid metabolism and their use to improve oil content

Cited by 3 publications

References 54 publications

Photoproduction of Aviation Fuel β‐Caryophyllene From the Eukaryotic Green Microalga Chlamydomonas reinhardtii

Photoproduction of Aviation Fuel β‐Caryophyllene From the Eukaryotic Green Microalga Chlamydomonas reinhardtii

Editorial overview: Plant synthetic biology

Microalgae Biotechnology: Methods and Applications

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