Advances in antimicrobial hydrogels for dental tissue engineering: regenerative strategies for endodontics and periodontics

Atila, Deniz; Kumaravel, Vignesh

doi:10.1039/d3bm00719g

Cited by 10 publications

(2 citation statements)

References 192 publications

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“…However, the results obtained show high anti-inflammatory activity, which guarantees biological effectiveness. Considering that infections are one of the main causes of dental health problems, using 3D-printed hydrogels with antimicrobial qualities has benefits for treating periodontal abnormalities [41]. The potential of the extract and CS against bacterial and fungal species inhabiting the human oral cavity, including pathogenic strains, has been previously assessed.…”

Section: Resultsmentioning

confidence: 99%

3D-Printed Chitosan-Based Scaffolds with Scutellariae baicalensis Extract for Dental Applications

Paczkowska-Walendowska,

Koumentakou,

Lazaridou

et al. 2024

Pharmaceutics

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The plant material Scutellariae baicalensis radix, which is rich in flavones (baicalin), possesses antibacterial, antifungal, antioxidant, and anti-inflammatory properties. This work aimed to develop a 3D-printed chitosan-based hydrogel rich in Scutellariae baicalensis extract as an innovative approach for the personalized treatment of periodontal diseases. Chitosan-based hydrogels were prepared, and the printability of the prepared hydrogels was determined. The hydrogel with 2.5% w/v of high molecular-weight chitosan (CS), 2% w/v gelatin (Gel), and 10% w/w of extract (Ex) presented the best printability, producing smooth and uniform scaffolds. It was proved that the CS/Gel/Ex hydrogel was stabilized by hydrogen bonds and remained in amorphous dispersion in the 3D-printed structures (confirmed by ATR-FTIR and XRPD). Due to the amorphization of the active substance, a significant increase in the release of baicalin in vitro was observed. It was demonstrated that there was an initial burst release and a continuous release profile (n = 3). Higuchi kinetic was the most likely baicalin release kinetic. The second fit, the Korsmeyer–Peppas kinetics model, showed coupled diffusion of the active ingredient in the hydrated matrix and polymer relaxation regulated release, with n values ranging from 0.45 to 0.89. The anti-inflammatory properties of 3D-printed scaffolds were assessed as the ability to inhibit the activity of the hyaluronidase enzyme. Activity was assessed as IC50 = 63.57 ± 4.98 mg hydrogel/mL (n = 6). Cytotoxicity tests demonstrated the biocompatibility of the material. After 24 h of exposure to the 2.5CS/2Gel/10Ex scaffold, fibroblasts migrated toward the scratch, closed the “wound” by 97.1%, and significantly accelerated the wound healing process. The results render the 3D-printed CS/Gel/extract scaffolds as potential candidates for treating periodontal diseases.

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Section: Resultsmentioning

confidence: 99%

3D-Printed Chitosan-Based Scaffolds with Scutellariae baicalensis Extract for Dental Applications

Paczkowska-Walendowska,

Koumentakou,

Lazaridou

et al. 2024

Pharmaceutics

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“…Additionally, hydrogel networks serve as effective drug delivery platforms owing to their high water retention capacity, enabling the incorporation of drugs or nanoparticles to confer antimicrobial properties and enhance regenerative potential. However, maintaining biocompatibility is crucial to avoid adverse effects [47,48].…”

Section: Bioprinted Scaffoldsmentioning

confidence: 99%

Biofabrication Approaches for Peri-Implantitis Tissue Regeneration: A Focus on Bioprinting Methods

Shopova,

Mihaylova,

Yaneva

et al. 2024

Prosthesis

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Dental implant utilization has emerged as a contemporary strategy for rectifying dental arch anomalies. However, the effective management of potential complications is paramount. Peri-implantitis, characterized by inflammation and bone resorption around dental implants, resembles periodontitis but specifically affects implant sites. Restoring lost peri-implant tissues poses a multifaceted challenge, with bioprinting methods showing promise as a viable solution. Three-dimensional bioprinting represents a forefront advancement in tissue engineering, traditionally focusing on scaffolds, cells, and signaling pathways. This systematic review aims to aggregate and synthesize data concerning bioprinting’s application in peri-implantitis treatment. Adhering to PRISMA guidelines, the review conducted an extensive literature search across PubMed, Scopus, Google Scholar, and ScienceDirect. Importantly, the search timeframe was not limited, reflecting the scarcity of available information on the subject. Bioprinting advancements offer auspicious avenues for refining treatment modalities, prompting clinicians to explore optimal solutions for establishing ideal anatomical conditions. In essence, this systematic review underscores 3D bioprinting’s potential in peri-implantitis management, highlighting its pivotal role in contemporary dental medicine and its capacity to reshape clinical approaches toward achieving optimal outcomes.

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Injectable Liposome‐Loaded Hydrogel Formulations with Controlled Release of Curcumin and α‐Tocopherol for Dental Tissue Engineering

Atila,

Dalgic,

Krzemińska

et al. 2024

Adv Healthcare Materials

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An injectable hydrogel formulation was developed utilizing low‐ and high‐molecular‐weight chitosan (LCH and HCH) incorporated with curcumin and α‐tocopherol‐loaded liposomes (Lip/Cur+Toc). Cur and Toc releases were delayed within the hydrogels. The injectability of hydrogels was proved via rheological analyses. In vitro studies were conducted using human dental pulp stem cells (hDPSCs) and human gingival fibroblasts (hGFs) to examine the biological performance of the hydrogels towards endodontics and periodontics, respectively. The viability of hDPSCs treated with the hydrogels with Lip/Cur+Toc was the highest till day 14, compared to the neat hydrogels. During odontogenic differentiation tests, ALP activity of hDPSCs was induced in the Cur‐containing groups. Biomineralization was enhanced mostly with Lip/Cur+Toc incorporation. The viability of hGFs was the highest in HCH combined with Lip/Cur+Toc while wound healing occurred almost 100% in both (Lip/Cur+Toc@LCH and Lip/Cur+Toc@HCH) after 2 days. Antioxidant activity of Lip/Cur+Toc@LCH on hGFs was significantly the highest among the groups. Antimicrobial tests demonstrated that Lip/Cur+Toc@LCH was more effective against Escherichia coli whereas so was Lip/Cur+Toc@HCH against Staphylococcus aureus. The antimicrobial mechanism of the hydrogels was investigated for the first time through various computational models. LCH and HCH loaded with Lip/Cur+Toc were promising candidates with multi‐functional features for endodontics and periodontics.This article is protected by copyright. All rights reserved

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Advances in antimicrobial hydrogels for dental tissue engineering: regenerative strategies for endodontics and periodontics

Abstract: Multifunctional hydrogels play a pivotal role against infections, inflammation, and oxidative stress, especially for endodontics and periodontics treatment.

Cited by 10 publications

References 192 publications

3D-Printed Chitosan-Based Scaffolds with Scutellariae baicalensis Extract for Dental Applications

3D-Printed Chitosan-Based Scaffolds with Scutellariae baicalensis Extract for Dental Applications

Biofabrication Approaches for Peri-Implantitis Tissue Regeneration: A Focus on Bioprinting Methods

Injectable Liposome‐Loaded Hydrogel Formulations with Controlled Release of Curcumin and α‐Tocopherol for Dental Tissue Engineering

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