Advances in banana transformation through<i>Agrobacterium tumefaciens</i>in Ecuador: progress, challenges and perspectives

Santos-Ordóñez, Efrén; Sánchez, E.; Hidalgo, L.; Chávez, T.; Villao, L.; Pacheco, Raquel; Flores, José; Korneva, S.; Navarrete, O.

doi:10.17660/actahortic.2016.1114.27

Cited by 9 publications

(5 citation statements)

References 11 publications

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“…The StSy gene was cloned under the control of the PR-10 promoter 11,21 . While under the control of the ubiquitin promoter, the Cu gene, Zn-SOD from tomato was cloned 14 . The terminators were inserted into the binary vector pGA 429 11 .…”

Section: Methods Used For Genetic Transformation Transformation Of Musa CV Grain Nain Mediated By Agrobacterium Tumefaciensmentioning

confidence: 99%

“…In banana Cavendish William it has been identified that the gene of chitinase is responsible for resistance to Mycosphaerella fijiensises 13 . To determine the research, it was carried out in a subtractive cDNA library after the inoculation of conidia in greenhouse conditions 14 . The vectors containing the promoters for the expression of the gene in banana are inserted in front of the reporter gene β-glucuronidase (uidAINT, Gus) of the plasmid Pcambia1391Z (pESKUL1 and pESKUL7) 15 .…”

Section: Identification Of Resistant Genes To the Black Sigatoka In Banana Musa Cavendish Aaamentioning

confidence: 99%

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Benefits in Latin America and the Caribbean about production of Cavendish AAA banana resistant to black Sigatoka

Torres¹,

Zamora²

2018

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Section: Methods Used For Genetic Transformation Transformation Of Musa CV Grain Nain Mediated By Agrobacterium Tumefaciensmentioning

confidence: 99%

Section: Identification Of Resistant Genes To the Black Sigatoka In Banana Musa Cavendish Aaamentioning

confidence: 99%

Benefits in Latin America and the Caribbean about production of Cavendish AAA banana resistant to black Sigatoka

Torres¹,

Zamora²

2018

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“…Genetic improvement arises as an essential strategy to overcome reduced productivity due to different stresses in the crops. Musa's conventional breeding is difficult due to different factors including high level of sterility, inrerploidy crosses, low rates of seed germination, and long crop cycle 2,3 .…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, genetic engineering techniques are alternative tools for the conventional breeding process, which may overcome some of the issues, including sterility and length, for the genetic improvement because several crosses should be performed in conventional breeding 2,3 . Therefore, the use of breeding technologies, including genetic engineering and recently, genome editing, has revolutionized genetic modification strategies for basic and applied science 4 .…”

Section: Introductionmentioning

confidence: 99%

“…Usually, six to twelve months could last to obtain transgenic in vitro banana plants after Agrobacterium-mediated transformation of embryogenic cell suspensions. Furthermore, the development of embryogenic cell suspensions could take eight to twelve months 2,3,5,6,7 . Therefore, the genetic transformation from in vitro banana plants was performed through Agrobacterium tumefaciens; and confirmation of transformation was performed by using the luciferase reporter gene system and PCR.…”

Section: Introductionmentioning

confidence: 99%

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Genetic transformation of apical meristematic shoots in the banana cultivar ‘Williams’

Villao

Flores

Santos-Ordóñez

2021

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Bananas and plantains (Musa spp.) are among the most critical socioeconomic crops globally, being a staple food for millions of people in the tropics and an essential component for the export market, including the subtropics. Besides conventional breeding, genetic improvement of bananas and plantains could be performed through genetic engineering and new breeding techniques. Furthermore, plant tissue culture is essential for these technologies, including developing embryogenic cell suspensions and in vitro plants. The transient and stable genetic transformation could be performed from in vitro plants, shortening Musa transgenic lines development compared to genetic transformation while using embryogenic cell suspension. In this study, a genetic transformation protocol was established from banana apical meristems for the ‘Williams’ cultivar (genotype AAA). The protocol was based on the co-cultivation of the explants (whole in vitro plants or bisected meristematic tissues derived from in vitro plants) with Agrobacterium tumefaciens strain LBA4404 harboring two binary vectors denominated pLVCIBE1 (cassette: MabHIPP promoter::luc2::Tnos, P35S::hpt::Tnos) and pLVCIBE2 (cassette: P35S::luc2::Tnos, P35S::hpt::Tnos), independently. The stable genetic transformation was obtained by subculturing in vitro banana plants in selection medium (12.5µg/mL of hygromycin) for 8 weeks from bisected meristematic tissue transformation. Genetic transformation was confirmed in vivo with the use of the luciferase reporter gene system. Furthermore, PCR was performed on DNA extracted from leaves of regenerated transgenic in vitro plants after 8 weeks of selection, confirming stable genetic transformation. Therefore, genetic transformation was achieved in the apical meristematic tissue of in vitro banana plants with co-cultivation of Agrobacterium tumefaciens.

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Complementation of Bananas Conventional Breeding Programs Through Biotechnological Genetic Improvement

López

Santos-Ordóñez

González

2020

Agricultural, Forestry and Bioindustry Biotechnology and Biodiscovery

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Advances in banana transformation throughAgrobacterium tumefaciensin Ecuador: progress, challenges and perspectives

Cited by 9 publications

References 11 publications

Benefits in Latin America and the Caribbean about production of Cavendish AAA banana resistant to black Sigatoka

Benefits in Latin America and the Caribbean about production of Cavendish AAA banana resistant to black Sigatoka

Genetic transformation of apical meristematic shoots in the banana cultivar ‘Williams’

Complementation of Bananas Conventional Breeding Programs Through Biotechnological Genetic Improvement

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