Advances in Bone Marrow-Derived Cell Therapy: CD31-expressing Cells as Next Generation Cardiovascular Cell Therapy

Kim, Sung Whan; Kim, Hyongbum; Yoon, Young Sup

doi:10.2217/rme.11.24

Cited by 25 publications

(18 citation statements)

References 136 publications

(178 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, BM-CD31 + cells induced the activation of angiogenic, anti-apoptotic and chemo-attractant factors in ischemic hind-limbs via a paracrine effect. Transplantation with BM-CD31 + cells significantly increased angiogenesis and improved recovery from ischemia 42,43. Jagged1 has been shown to drive immature BM progenitor cells to differentiate into functional EPCs [44].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Angiopoietin-1 Increases CD133+/c-kit+ Cells and Reduces Myocardial Apoptosis in db/db Mouse Infarcted Hearts

Zeng

Chen

2012

PLoS ONE

View full text Add to dashboard Cite

Hematopoietic progenitor CD133+/c-kit+ cells have been shown to be involved in myocardial healing following myocardial infarction (MI). Previously we demonstrated that angiopoietin-1(Ang-1) is beneficial in the repair of diabetic infarcted hearts. We now investigate whether Ang-1 affects CD133+/c-kit+ cell recruitment to the infarcted myocardium thereby mediating cardiac repair in type II (db/db) diabetic mice. db/db mice were administered either adenovirus Ang-1 (Ad-Ang-1) or Ad-β-gal systemically immediately after ligation of the left anterior descending coronary artery (LAD). Overexpression of Ang-1 resulted in a significant increase in CXCR-4/SDF-1α expression and promoted CD133+/c-kit+, CD133+/CXCR-4+ and CD133+/SDF-1α+ cell recruitment into ischemic hearts. Overexpression of Ang-1 led to significant increases in number of CD31+ and smooth muscle-like cells and VEGF expression in bone marrow (BM). This was accompanied by significant decreases in cardiac apoptosis and fibrosis and an increase in myocardial capillary density. Ang-1 also upregulated Jagged-1, Notch3 and apelin expression followed by increases in arteriole formation in the infarcted myocardium. Furthermore, overexpression of Ang-1 resulted in a significant improvement of cardiac functional recovery after 14 days of ischemia. Our data strongly suggest that Ang-1 attenuates cardiac apoptosis and promotes cardiac repair by a mechanism involving in promoting CD133+/c-kit+ cells and angiogenesis in diabetic db/db mouse infarcted hearts.

show abstract

Section: Discussionmentioning

confidence: 99%

Overexpression of Angiopoietin-1 Increases CD133+/c-kit+ Cells and Reduces Myocardial Apoptosis in db/db Mouse Infarcted Hearts

Zeng

Chen

2012

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…With respect to ECs, CD31 has been shown to be involved in cell-to-cell contacts, providing a role in the maintenance of vascular integrity [1]. With respect to neutrophils, monocytes, and progenitor cells, CD31 is involved in mediating trans-endothelial migration, a key step in both inflammation and angiogenesis [2-4]. CD31 has been shown to promote interactions between ECs and monocytes, including the activation of antiapoptotic pathways [5].…”

mentioning

confidence: 99%

Circulating CD31+ leukocyte frequency is associated with cardiovascular risk factors

Cheng

Larson

et al. 2013

Atherosclerosis

View full text Add to dashboard Cite

Objectives CD31 identifies a heterogeneous population of cells in the blood, consisting of mature leukocytes and platelets, as well as smaller numbers of endothelial and progenitor cells. Because unfractionated CD31+ blood cells have demonstrated angiogenic properties in vivo, we hypothesized that circulating CD31+ cells would be related to the presence of cardiovascular risk factors in humans. Methods and Results We studied 1,487 participants, free of cardiovascular disease, from the Framingham Offspring Study. Using anti-human CD31 and CD45 antibodies, distinct CD31+/CD45+ leukocyte populations were enumerated in blood samples by FACS analysis. We used linear regression analyses to investigate the relation of each cell phenotype with cardiovascular risk factors. We identified 3 distinct leukocyte populations: CD31-, CD31dim, and CD31bright cells. Using forward/side scatter analyses, CD31- and CD31dim cells mapped to lymphoid gates while CD31bright cells were monocytoid. In multivariable analyses, higher frequency of CD31bright cells was associated with older age, male sex, and CRP (all P<0.001). In contrast, CD31dim was inversely associated with age, male sex, CRP, and smoking (all P<0.01). Framingham Risk Score was positively associated with CD31bright frequency (P=0.002), and negatively associated with CD31dim frequency (P=0.020). Conclusions CD31+ staining identifies 2 major leukocyte populations, CD31bright and CD31dim, which demonstrated significant and opposite associations with cardiovascular risk in humans. Further research is needed to define the biological and potential therapeutic roles of CD31+ subpopulations in vascular disease.

show abstract

“…There are numerous reports that human ASCs (hASCs) can both differentiate into endothelial lineage cells in vitro and have a proangiogenic action in ischemia models (Miranville et al, 2004; Planat‐Benard et al, 2004; Ning et al, 2009). Postnatal EPCs and MSCs are found only at low levels in BM and circulation, therefore, limiting their therapeutic application in regenerative medicine, such as cell therapy and tissue engineering (Kim et al, 2011). However, hASCs are regarded as an attractive cell source for therapeutic angiogenesis because large numbers are easily obtained.…”

mentioning

confidence: 99%

Endothelial Differentiation and Vasculogenesis Induced by Three‐Dimensional Adipose‐Derived Stem Cells

Park

Kim

Jung

et al. 2012

The Anatomical Record

View full text Add to dashboard Cite

Recently, an angiogenic therapy based on adipose-derived stem cells (ASCs) in an ischemic model has been reported. This study demonstrates the differentiation of human ASCs (hASCs) into endothelial cells clusters by culturing the cells in the form of three dimensional cell masses (3DCMs), which is based on the adherent activity of ASCs for a substrate. The 3DCM composed of hASCs induced hypoxic conditions and expressed angiogenic factors, such as vascular endothelial growth factor and interleukin-8. From immunochemical staining analysis, the 3DCMs of hASCs were CD31 þ , KDR þ , and CD34 þ , whereas monolayer-cultured hASCs were negative for the these markers. To evaluate the ability of vasculature to form within 3DCMs, the 3DCMs were mixed in Matrigel/fibrin gel and injected into mice. Mature tubular microvessels perfused with blood were observed in the 3DCM/gel 20 days after injection, but not in the gel alone or hASC/ gel mixture. Vasculature formed in the 3DCM/gel was recognized by antibodies against human a-smooth muscle actin, KDR, CD31, and CD34, but not by antibodies against murine antigens. These results suggest that the vasculatures originated from the embedded human cells. The 3DCMs of hASCs could function as a source of vascular cells for neovascularization, and could also be co-implanted with other cell types for regenerative medicine. Anat Rec,

show abstract

Advances in Bone Marrow-Derived Cell Therapy: CD31-expressing Cells as Next Generation Cardiovascular Cell Therapy

Cited by 25 publications

References 136 publications

Overexpression of Angiopoietin-1 Increases CD133+/c-kit+ Cells and Reduces Myocardial Apoptosis in db/db Mouse Infarcted Hearts

Overexpression of Angiopoietin-1 Increases CD133+/c-kit+ Cells and Reduces Myocardial Apoptosis in db/db Mouse Infarcted Hearts

Circulating CD31+ leukocyte frequency is associated with cardiovascular risk factors

Endothelial Differentiation and Vasculogenesis Induced by Three‐Dimensional Adipose‐Derived Stem Cells

Contact Info

Product

Resources

About