Advances in Breast Cancer Therapy Using Nitric Oxide and Nitroxyl Donor Agents

Basudhar, Debashree; Miranda, Katrina M.; Wink, David A.; Ridnour, Lisa A.

doi:10.1007/978-3-319-30705-3_15

Cited by 4 publications

(3 citation statements)

References 201 publications

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“…Nitric oxide (NO) regulates posttranslational modifications, S-nitrosation, as well as genome-wide epigenetic modifications that can have both tumor-promoting and tumor-suppressing effects [33]. These effects have been described to be concentration-dependent with low NO concentrations being associated with chemo-resistance, anti-apoptosis, proliferation, metastasis, reduced immune response and angiogenesis while high NO is associated with apoptosis, anti-proliferation, anti-angiogenesis, anti-metastasis, and immune response [34,35]. Interestingly, in blood of breast cancer patients, high levels of NO have been detected and increased nitric oxide synthase (NOS) activity in invasive breast tumors compared to benign or normal breast tissue, suggesting a positive correlation between NO biosynthesis and degree of malignancy [36,37].…”

Section: Selectivity Of Cap Towards Malignant Cellsmentioning

confidence: 99%

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Semmler

Bekeschus

Schäfer

et al. 2020

Cancers

157

127

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Recently, the potential use of cold atmospheric pressure plasma (CAP) in cancer treatment has gained increasing interest. Especially the enhanced selective killing of tumor cells compared to normal cells has prompted researchers to elucidate the molecular mechanisms for the efficacy of CAP in cancer treatment. This review summarizes the current understanding of how CAP triggers intracellular pathways that induce growth inhibition or cell death. We discuss what factors may contribute to the potential selectivity of CAP towards cancer cells compared to their non-malignant counterparts. Furthermore, the potential of CAP to trigger an immune response is briefly discussed. Finally, this overview demonstrates how these concepts bear first fruits in clinical applications applying CAP treatment in head and neck squamous cell cancer as well as actinic keratosis. Although significant progress towards understanding the underlying mechanisms regarding the efficacy of CAP in cancer treatment has been made, much still needs to be done with respect to different treatment conditions and comparison of malignant and non-malignant cells of the same cell type and same donor. Furthermore, clinical pilot studies and the assessment of systemic effects will be of tremendous importance towards bringing this innovative technology into clinical practice.

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Section: Selectivity Of Cap Towards Malignant Cellsmentioning

confidence: 99%

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Semmler

Bekeschus

Schäfer

et al. 2020

Cancers

157

127

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show abstract

“…NO can act as an intra- and extracellular signaler participating in numerous pathological processes. It is a modulator in several essential biological processes, and it has a dubious role in cells, sometimes beneficial and sometimes harmful [ 28 ]. Also, NO is an important cytotoxic mediator of effector cells capable of destroying pathogens and tumor cells [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Parasporin A13-2 of Bacillus thuringiensis Isolates from the Papaloapan Region (Mexico) Induce a Cytotoxic Effect by Late Apoptosis against Breast Cancer Cells

Borin

Castrejón-Arroyo

Cruz-Nolasco

et al. 2021

Toxins

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The protein A13-2 was obtained from Bacillus thuringiensis strains isolated from the Papaloapan watershed region (Oaxaca, Mexico). The cytotoxic activity of parasporal inclusions was studied against breast cancer cell line (MCF-7) and normal cell (human peripheral blood mononuclear cells). The MTT, the formation of reactive species, nitric oxide, free cell DNA, and the type of death cellular were assessed. The protein A13-2 shows the highest cytotoxic activity against MCF-7 (13% cell viability at 6 µg/mL), the extracellular DNA increases, and it shows no stress for reactive species or nitric oxide. Besides, the A13-2 parasporin shows no toxicity to peripheral blood mononuclear cells, and it does not generate changes in nitric oxide levels or free cell DNA. Due to that, the cytotoxic effect of A13-2 was specific for MCF-7, and it does not affect normal cells. According to microscopy and flow cytometry, A13-2 parasporin leads to the death of MCF-7 cells by late apoptosis together with necrosis and without allowing the triggering of the survival mechanisms. When analyzed together, our results show for the first time that the A13-2 protein isolated from Mexican strains of B. thuringiensis preferentially kills MCF- 7 (cancer cells) over HEK 293 and PBMC cell lines (normal cells), thus representing a promising alternative for the treatment of cancer breast.

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“…NO can act as an intraand extracellular signaler participating in numerous pathological processes. It is a modulator in several essential biological processes, and it has a dubious role to cells, sometimes beneficial and sometimes harmful [26]. Also, NO is an important cytotoxic mediator of effector cells capable of destroying pathogens and tumor cells [27].…”

Section: Morphological Changes and Cell Death Mechanism Induced By Pamentioning

confidence: 99%

Parasporin A13-2 of Bacillus thuringiensis isolate from Papaloapan region, induce a cytotoxic effect by late apoptosis against breast cancer cells

Borin

Castrejón-Arroyo

Cruz-Nolasco

et al. 2021

Preprint

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The protein A13-2 was obtained from Bacillus thuringiensis strains isolated from the Papaloapan watershed region (Oaxaca, Mexico). The cytotoxic activity of parasporal inclusions was studied against breast cancer cell line (MCF-7) and normal cell (human peripheral blood mononuclear cells). The MTT, the formation of reactive species, nitric oxide, free cell DNA, and the type of death cellular were assessed. The protein A13-2 shows the highest cytotoxic activity against MCF-7 (13% at 6 µg/mL), the extracellular DNA increases, and it shows no stress for reactive species or nitric oxide. Besides, the A13-2 parasporin shows no toxicity to peripheral blood mononuclear cells, and it does not generate changes in nitric oxide levels or free cell DNA. According to microscopy and flow cytometry, A13-2 leads to the death of MCF-7 cell by late apoptosis. Due to that, the cytotoxic effect of A13-2 was specific for MCF-7, and it does not affect peripheral blood mononuclear cells (normal cells). When analyzed together, our results show for the first time that the A13-2 protein isolated from Mexican strains of B. thuringiensis has a high selectivity against the MCF- 7 cell line, thus representing a promising alternative for the treatment of cancer breast.

show abstract

Advances in Breast Cancer Therapy Using Nitric Oxide and Nitroxyl Donor Agents

Cited by 4 publications

References 201 publications

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Parasporin A13-2 of Bacillus thuringiensis Isolates from the Papaloapan Region (Mexico) Induce a Cytotoxic Effect by Late Apoptosis against Breast Cancer Cells

Parasporin A13-2 of Bacillus thuringiensis isolate from Papaloapan region, induce a cytotoxic effect by late apoptosis against breast cancer cells

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