Advances in CRISPR/Cas9-mediated genome editing on vegetable crops

Tian, Shouwei; Xing, Sinian; Xu, Yong

doi:10.1007/s11627-021-10187-z

Cited by 11 publications

(6 citation statements)

References 137 publications

(96 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transgenic breeding surely provides an alternative by creating genetically modified crops with desired traits in less time but their use is strictly restricted or legally prohibited by government agencies of several countries under safety regulations (Voytas and Gao, 2014). The discovery of genome-editing technology, particularly CRISPR/Cas, has enabled researchers to make precise modifications at specific sites in the DNA to generate horticultural crops possessing novel and desired traits within a short time (Tian et al, 2021) (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Genetic amelioration of fruit and vegetable crops to increase biotic and abiotic stress resistance through CRISPR Genome Editing

Sardar

2023

Front. Plant Sci.

View full text Add to dashboard Cite

Environmental changes and increasing population are major concerns for crop production and food security as a whole. To address this, researchers had focussed on the improvement of cereals and pulses and have made considerable progress till the beginning of this decade. However, cereals and pulses together, without vegetables and fruits, are inadequate to meet the dietary and nutritional demands of human life. Production of good quality vegetables and fruits is highly challenging owing to their perishable nature and short shelf life as well as abiotic and biotic stresses encountered during pre- and post-harvest. Genetic engineering approaches to produce good quality, to increase shelf life and stress-resistance, and to change the time of flowering and fruit ripening by introducing foreign genes to produce genetically modified crops were quite successful. However, several biosafety concerns, such as the risk of transgene-outcrossing, limited their production, marketing, and consumption. Modern genome editing techniques, like the CRISPR/Cas9 system, provide a perfect solution in this scenario, as it can produce transgene-free genetically edited plants. Hence, these genetically edited plants can easily satisfy the biosafety norms for crop production and consumption. This review highlights the potential of the CRISPR/Cas9 system for the successful generation of abiotic and biotic stress resistance and thereby improving the quality, yield, and overall productivity of vegetables and fruits.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic amelioration of fruit and vegetable crops to increase biotic and abiotic stress resistance through CRISPR Genome Editing

Sardar

2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Non-homologous end joining results in knockout alleles through frameshift mutations due to the loss of nucleotides, while HDR leads to the introduction of precise genetic modifications, including single-nucleotide substitutions, gene replacements, and large insertions ( Huang and Puchta, 2019 ). The CRISPR-Cas9 system has been successfully employed to modify cereal, vegetable, and horticultural plant species to improve their agronomic traits ( Xu et al, 2019 ; Ansari et al, 2020 ; Tian, et al, 2021 ). The challenges in sugarcane, being of a polyploid nature with a large number of homeologs and homologs, could have functional redundancy of alleles.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Sugarcane Genomics, Physiology, and Phenomics for Superior Agronomic Traits

et al. 2022

View full text Add to dashboard Cite

Advances in sugarcane breeding have contributed significantly to improvements in agronomic traits and crop yield. However, the growing global demand for sugar and biofuel in the context of climate change requires further improvements in cane and sugar yields. Attempts to achieve the desired rates of genetic gain in sugarcane by conventional breeding means are difficult as many agronomic traits are genetically complex and polygenic, with each gene exerting small effects. Unlike those of many other crops, the sugarcane genome is highly heterozygous due to its autopolyploid nature, which further hinders the development of a comprehensive genetic map. Despite these limitations, many superior agronomic traits/genes for higher cane yield, sugar production, and disease/pest resistance have been identified through the mapping of quantitative trait loci, genome-wide association studies, and transcriptome approaches. Improvements in traits controlled by one or two loci are relatively easy to achieve; however, this is not the case for traits governed by many genes. Many desirable phenotypic traits are controlled by quantitative trait nucleotides (QTNs) with small and variable effects. Assembling these desired QTNs by conventional breeding methods is time consuming and inefficient due to genetic drift. However, recent developments in genomics selection (GS) have allowed sugarcane researchers to select and accumulate desirable alleles imparting superior traits as GS is based on genomic estimated breeding values, which substantially increases the selection efficiency and genetic gain in sugarcane breeding programs. Next-generation sequencing techniques coupled with genome-editing technologies have provided new vistas in harnessing the sugarcane genome to look for desirable agronomic traits such as erect canopy, leaf angle, prolonged greening, high biomass, deep root system, and the non-flowering nature of the crop. Many desirable cane-yielding traits, such as single cane weight, numbers of tillers, numbers of millable canes, as well as cane quality traits, such as sucrose and sugar yield, have been explored using these recent biotechnological tools. This review will focus on the recent advances in sugarcane genomics related to genetic gain and the identification of favorable alleles for superior agronomic traits for further utilization in sugarcane breeding programs.

show abstract

“…Over the last few decades, there have been a large number of significant developments in the molecular biology approaches to improve crop yield and quality. Recently, tremendous progress has been made in genome editing tools like site-directed nucleases (SDNs) which are able to edit the crops at high speed and possess great potential in shaping up the novel genetic makeup of vegetable crops ( Tian et al, 2021 ). Genome editing tools can precisely engineer the genes by either deleting, replacing or inserting specific sequences at the specific targeted location in the target genome to generate novel traits ( Corte et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Genome editing for vegetable crop improvement: Challenges and future prospects

2022

View full text Add to dashboard Cite

Vegetable crops are known as protective foods due to their potential role in a balanced human diet, especially for vegetarians as they are a rich source of vitamins and minerals along with dietary fibers. Many biotic and abiotic stresses threaten the crop growth, yield and quality of these crops. These crops are annual, biennial and perennial in breeding behavior. Traditional breeding strategies pose many challenges in improving economic crop traits. As in most of the cases the large number of backcrosses and stringent selection pressure is required for the introgression of the useful traits into the germplasm, which is time and labour-intensive process. Plant scientists have improved economic traits like yield, quality, biotic stress resistance, abiotic stress tolerance, and improved nutritional quality of crops more precisely and accurately through the use of the revolutionary breeding method known as clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein-9 (Cas9). The high mutation efficiency, less off-target consequences and simplicity of this technique has made it possible to attain novel germplasm resources through gene-directed mutation. It facilitates mutagenic response even in complicated genomes which are difficult to breed using traditional approaches. The revelation of functions of important genes with the advancement of whole-genome sequencing has facilitated the CRISPR-Cas9 editing to mutate the desired target genes. This technology speeds up the creation of new germplasm resources having better agro-economical traits. This review entails a detailed description of CRISPR-Cas9 gene editing technology along with its potential applications in olericulture, challenges faced and future prospects.

show abstract

Advances in CRISPR/Cas9-mediated genome editing on vegetable crops

Cited by 11 publications

References 137 publications

Genetic amelioration of fruit and vegetable crops to increase biotic and abiotic stress resistance through CRISPR Genome Editing

Genetic amelioration of fruit and vegetable crops to increase biotic and abiotic stress resistance through CRISPR Genome Editing

Recent Advances in Sugarcane Genomics, Physiology, and Phenomics for Superior Agronomic Traits

Genome editing for vegetable crop improvement: Challenges and future prospects

Contact Info

Product

Resources

About