Advances in CRISPR/Cas9

Zhu, Youmin

doi:10.1155/2022/9978571

Cited by 28 publications

(13 citation statements)

References 160 publications

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“…The application of CRISPR/ Cas 9 technology to inject guide RNA (gRNA) and Cas 9 mRNA at the single-cell embryonic stage can quickly obtain gene knockout zebrafish, which provides an ideal model for the study of pancreatic diseases and glucose metabolism. It is an effective technical means to construct diabetic zebrafish models [ 99 , 100 , 101 , 102 ].…”

Section: Methods Of Diabetic Zebrafish Model Constructionmentioning

confidence: 99%

Research Progress on the Construction and Application of a Diabetic Zebrafish Model

Cao

Chen

Liu

et al. 2023

IJMS

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Diabetes is a metabolic disease characterized by high blood glucose levels. With economic development and lifestyle changes, the prevalence of diabetes is increasing yearly. Thus, it has become an increasingly serious public health problem in countries around the world. The etiology of diabetes is complex, and its pathogenic mechanisms are not completely clear. The use of diabetic animal models is helpful in the study of the pathogenesis of diabetes and the development of drugs. The emerging vertebrate model of zebrafish has many advantages, such as its small size, large number of eggs, short growth cycle, simple cultivation of adult fish, and effective improvement of experimental efficiency. Thus, this model is highly suitable for research as an animal model of diabetes. This review not only summarizes the advantages of zebrafish as a diabetes model, but also summarizes the construction methods and challenges of zebrafish models of type 1 diabetes, type 2 diabetes, and diabetes complications. This study provides valuable reference information for further study of the pathological mechanisms of diabetes and the research and development of new related therapeutic drugs.

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Section: Methods Of Diabetic Zebrafish Model Constructionmentioning

confidence: 99%

Research Progress on the Construction and Application of a Diabetic Zebrafish Model

Cao

Chen

Liu

et al. 2023

IJMS

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“…The three most advanced GED technologies ( Figure 1 ) are zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR-Cas-associated nucleases (CRISPR/Cas9) ( Gaj et al, 2013 ; Gaj et al, 2016 ; Siva et al, 2021 ). CRISPR/Cas9 is the most commonly used GED technology due to its versatility, effectiveness, and ease of use ( Zhu, 2022 ; Adli, 2018 ; Arora and Narula, 2017 ). The cell and gene therapy sector are constantly evolving, and recent years have seen remarkable progress in the creation of CRISPR-based treatments, leading to the commencement of numerous clinical trials ( CTG Labs - NCBI, 2023a ; CTG Labs - NCBI, 2023b ; CTG Labs - NCBI, 2023c ; CTG Labs - NCBI, 2023d ).…”

Section: Introductionmentioning

confidence: 99%

Advancing genome editing with artificial intelligence: opportunities, challenges, and future directions

Dixit,

Kumar,

Srinivasan

et al. 2024

Front. Bioeng. Biotechnol.

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Clustered regularly interspaced short palindromic repeat (CRISPR)-based genome editing (GED) technologies have unlocked exciting possibilities for understanding genes and improving medical treatments. On the other hand, Artificial intelligence (AI) helps genome editing achieve more precision, efficiency, and affordability in tackling various diseases, like Sickle cell anemia or Thalassemia. AI models have been in use for designing guide RNAs (gRNAs) for CRISPR-Cas systems. Tools like DeepCRISPR, CRISTA, and DeepHF have the capability to predict optimal guide RNAs (gRNAs) for a specified target sequence. These predictions take into account multiple factors, including genomic context, Cas protein type, desired mutation type, on-target/off-target scores, potential off-target sites, and the potential impacts of genome editing on gene function and cell phenotype. These models aid in optimizing different genome editing technologies, such as base, prime, and epigenome editing, which are advanced techniques to introduce precise and programmable changes to DNA sequences without relying on the homology-directed repair pathway or donor DNA templates. Furthermore, AI, in collaboration with genome editing and precision medicine, enables personalized treatments based on genetic profiles. AI analyzes patients’ genomic data to identify mutations, variations, and biomarkers associated with different diseases like Cancer, Diabetes, Alzheimer’s, etc. However, several challenges persist, including high costs, off-target editing, suitable delivery methods for CRISPR cargoes, improving editing efficiency, and ensuring safety in clinical applications. This review explores AI’s contribution to improving CRISPR-based genome editing technologies and addresses existing challenges. It also discusses potential areas for future research in AI-driven CRISPR-based genome editing technologies. The integration of AI and genome editing opens up new possibilities for genetics, biomedicine, and healthcare, with significant implications for human health.

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“…The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein (CRISPR/Cas) system is an inheritable adaptive immune system found in both archaeal and bacterial organisms. It is broadly classified into two major classes encompassing six types and several subtypes ( Zhu, 2022 ; Mishra et al, 2023 ). Within this system, class I utilizes multiple effector proteins, while the class II system employs a single effector protein for target interference.…”

Section: Introductionmentioning

confidence: 99%

“…Within this system, class I utilizes multiple effector proteins, while the class II system employs a single effector protein for target interference. One of the most well-characterized and commonly used systems within the CRISPR/Cas family is the type II, class II CRISPR system that relies on a single Cas9 protein ( Zhu, 2022 ; Mishra et al, 2023 ). Mechanistically, the induction of double-stranded DNA break (DSB) is achieved by the binding of Cas9 endonuclease to the target DNA sequence guided by the single-guide RNA (sgRNA), a fusion of trans-activating RNA (tracrRNA) and CRISPR-targeting RNA (crRNA).…”

Section: Introductionmentioning

confidence: 99%

Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling

Ravichandran,

Maddalo

2023

Front. Genet.

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The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9) system is a powerful tool that enables precise and efficient gene manipulation. In a relatively short time, CRISPR has risen to become the preferred gene-editing system due to its high efficiency, simplicity, and programmability at low costs. Furthermore, in the recent years, the CRISPR toolkit has been rapidly expanding, and the emerging advancements have shown tremendous potential in uncovering molecular mechanisms and new therapeutic strategies for human diseases. In this review, we provide our perspectives on the recent advancements in CRISPR technology and its impact on precision medicine, ranging from target identification, disease modeling, and diagnostics. We also discuss the impact of novel approaches such as epigenome, base, and prime editing on preclinical cancer drug discovery.

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Advances in CRISPR/Cas9

Cited by 28 publications

References 160 publications

Research Progress on the Construction and Application of a Diabetic Zebrafish Model

Research Progress on the Construction and Application of a Diabetic Zebrafish Model

Advancing genome editing with artificial intelligence: opportunities, challenges, and future directions

Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling

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