Advances in cryopreservation of vanilla (Vanilla planifolia Jacks.) shoot-tips: assessment of new biotechnological and cryogenic factors

Hernández-Ramírez, Fabiola; Dolce, Natalia Raquel; Flores-Castaños, O.; Rascón, M.P.; Angeles, Guillermo; Folgado, Raquel; González-Arnao, María Teresa

doi:10.1007/s11627-020-10069-w

Cited by 8 publications

(9 citation statements)

References 18 publications

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“…The results after the cryopreservation of vanilla shoot-tips were very similar to that previously reported by Hernández-Ramírez et al [11] and González-Arnao et al [1], using the same three procedures. The post-cryopreservation recovery was quite variable, except when the D-Cp protocol was used.…”

Section: Cryopreservation Of Shoot Tips From In Vitro-grown Plantssupporting

confidence: 89%

“…Based on the overall results, D-Cp appears to be the most recommendable cryopreservation method for V. planifolia. According to Hernández-Ramírez et al [11], this procedure is the most optimized and reproducible for shoot tips of this vanilla species. As observed here, the greenhouse-grown regenerants produced using this technique showed more vigorous vegetative growth and low levels of polymorphism (average 27%).…”

Section: Discussionmentioning

confidence: 99%

“…Different biotechnological techniques, such as tissue culture [5][6][7], temporary immersion bioreactors [8,9], genetic transformation [10] and cryopreservation [11,12] have been studied for the in vitro propagation and conservation of V. planifolia germplasm. For instance, the cryopreservation of vanilla shoot-tips was reported for the first time in 2009 [12] and since then, different cryo-biotechnological investigations have been carried out to optimize and refine a robust and reproducible protocol [11].…”

Section: Introductionmentioning

confidence: 99%

“…Vitrification-based procedures, such as droplet-vitrification (D-V) [12], V-cryoplate (V-Cp) [1] and D-cryoplate (D-Cp) [11] led to differential survival and regeneration percentages following the cryopreservation of V. planifolia shoot-tips. Using D-V, the main problem was associated with oxidative damage [12].…”

Section: Introductionmentioning

confidence: 99%

“…Using D-V, the main problem was associated with oxidative damage [12]. Following the V-Cp and D-Cp methods, the difficulties were associated with the transient de-differentiation of tissues after their exposure to liquid nitrogen [1,11]. However, despite some detrimental effects on survival Plants 2022, 11, 1630 2 of 13 and recovery, as mentioned above, all techniques allowed regrowth in various percentages of cryopreserved vanilla explants.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Vegetative Growth and Genetic Integrity of Vanilla planifolia Regenerants after Cryopreservation

González-Arnao

Cruz‐Cruz

Hernández-Ramírez

et al. 2022

Plants

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Vanilla planifolia Jacks. ex Andrews is the vanilla species with the most commercial and greatest economic importance. It has been used as a case study in different cryopreservation studies that involve three vitrification-based approaches: droplet-vitrification (D-V), V-cryoplate (V-Cp) and D-cryoplate (D-Cp). The aim of this study was to compare the impact of these cryogenic techniques on vegetative growth (survival, stem length and leaf number) between cryo-derived plants and in vitro-derived controls during 12 months of greenhouse growth. Genetic stability was also assessed using the inter-simple sequence repeat (ISSR) markers. There were no significant differences found in the survival and stem lengths of the in vitro-derived regenerants and cryo-derived plants. A significant increase in the number of leaves was only detected in cryo-derived plants when using the V-Cp method. The electrophoretic profiles, based on seven ISSR primers, detected low variability: 81 total bands and 27% polymorphism. This is the first report on the assessment of vegetative growth and genetic integrity in cryo-derived V. planifolia plants recovered under greenhouse conditions. Of the three cryogenic approaches, D-Cp appears to yield V. planifolia regenerants plants with more vigorous vegetative growth and a lower level of polymorphism. Future research should focus on the reproductive growth of vanilla regenerants.

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Section: Cryopreservation Of Shoot Tips From In Vitro-grown Plantssupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%