Advances in Desert Truffle Mycorrhization and Cultivation

Morte, Asunción; Gutiérrez, Almudena; Ródenas, Alfonso Navarro

doi:10.1007/978-3-030-37378-8_7

Cited by 9 publications

(11 citation statements)

References 33 publications

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“…The symbiotic relationship between desert truffles and their host plants was the key for the domestication of these wild edible mushrooms through the production and field transplantation of the desert truffles- Helianthemum mycorrhizal seedlings ( Slama et al, 2010 ; Morte, Honrubia & Gutiérrez, 2008 ; Morte et al, 2009 ; Morte et al, 2012 ; Morte et al, 2017 ; Morte, Gutiérrez & Ródenas, 2020 ). Since the first Terfezia ascomata were produced from a plantation of Helianthemum almeriense mycorrhizal plants in Spain, an increasing demand for this crop has prompted research into new strategies to enhance the production of these fungi.…”

Section: Introductionmentioning

confidence: 99%

Growth promotion and mycorrhizal colonization of Argan (Argania spinosa (L.) Skeels) inoculated with the edible desert truffle Tirmania nivea (Desf.) Trappe

Khrizi

Zitouni-Haouar

Fortas

2022

PeerJ

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This study presents the first evidence of the mycorrhizal compatibility between the edible desert truffle Tirmania nivea and the valuable fruit tree Argania spinosa. Seed germination trials demonstrated that soaking pre-treatment of argan seeds in hydrogen peroxide (9%) for five days combined with the application of a fungicide treatment on an inert sowing material maximized the seed germination of this tree species. The mycorrhizal synthesis was conducted under greenhouse conditions by inoculating, in vivo, the host plant seedlings with spores of T. nivea. The growth and mycorrhizal status of A. spinosa was assessed 15.5 months after inoculation. The desert truffle mycorrhization significantly promoted all the investigated morphological parameters of growth and improved the physiological performances of the host plant through enhancing plant water status and chlorophyll concentration. The mycorrhizal symbiosis led to the formation of typical desert truffle endomycorrhizae with intracellular coils. The resistance of A. spinosa to the harsh environmental conditions of desert habitats makes it a potential candidate for cultivation of desert truffles.

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Section: Introductionmentioning

confidence: 99%

Growth promotion and mycorrhizal colonization of Argan (Argania spinosa (L.) Skeels) inoculated with the edible desert truffle Tirmania nivea (Desf.) Trappe

Khrizi

Zitouni-Haouar

Fortas

2022

PeerJ

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“…Its natural habitats are arid and semiarid environments with a low annual rainfall inputs, mild winters, and warm summers, mainly encompassing countries of the Mediterranean geographical region [ 2 , 3 ]. T. claveryi was the first desert truffle to be cultivated [ 4 ], and it is known to be one of the most appreciated desert truffle species on the market [ 5 ], together with other known desert truffles (mainly Terfezia boudieri Chatin, Tirmania nivea (Desf.) Trappe and Tirmania pinoyi (Maire) Malençon [ 6 ]).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the area cultivated with the desert truffle T. claveryi has been increased in semiarid areas of Spain [ 5 , 13 ], becoming an alternative agricultural crop thanks to low water requirements for cultivation [ 14 ]. Until now, some abiotic factors or agroclimatic parameters associated with plant management and the control of fungal fruiting have been studied [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…Soil properties such as pH (acid or alkaline) and the host plant species lead to the fruiting of different species of desert truffle [ 1 , 5 ]. In recent years, several studies on the genus Terfezia have been published to clarify and update the phylogenetic relationships among the new species and those already described within the genus [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

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Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil

Arenas

Morte

Navarro‐Ródenas

2022

JoF

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Desert truffle crop is a pioneer in southeastern Spain, a region where native edible hypogeous fungi are adapted to the semiarid areas with low annual rainfall. Terfezia claveryi Chatin was the first species of desert truffle to be cultivated, and has been increasing in recent years as an alternative rainfed crop in the Iberian Peninsula. However, its behaviour in the field has yet not been investigated. For this purpose, specific primers were designed for the soil DNA quantification of both T. claveryi and Terfezia crassiverrucosa and a real-time qPCR protocol was developed, using the ITS rDNA region as a target. Moreover, a young desert truffle orchard was sampled for environmental validation. The results showed the highest efficiency for the TerclaF3/TerclaR1 primers pair, 89%, and the minimal fungal biomass that could be reliable detected was set at 4.23 µg mycelium/g soil. The spatial distribution of fungal biomass was heterogeneous, and there was not a direct relationship between the quantity of winter soil mycelium and the location/productivity of desert truffles. This protocol could be applied to tracking these species in soil and understand their mycelial dynamics in plantations and wild areas.

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“…Moreover, this crop could play an important ecological role in arid and semi‐arid ecosystems as natural desert truffle resource (sylviculture), conserving these areas from desertification or climate change processes (Honrubia et al ., 2014). The main difficulties for its cultivation are the fluctuations of the ascocarps production over the years (Morte et al ., 2017), in which 1 year is highly productive and the next has almost no truffle production (Morte et al ., 2012, 2020).…”

Section: Introductionmentioning

confidence: 99%

Different patterns in root and soil fungal diversity drive plant productivity of the desert truffle Terfezia claveryi in plantation

Arenas

Navarro‐Ródenas

Marqués‐Gálvez

et al. 2021

Environmental Microbiology

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The desert truffle Terfezia claveryi is one of the few mycorrhizal fungi currently in cultivation in semiarid and arid areas. Agroclimatic parameters seem to affect its annual yield, but there is no information on the influence of biotic factors. In this study, fungal diversity was analysed by high-throughput sequencing of the ITS2 rDNA region from soil and root samples to compare productive and non-productive mycorrhizal plants in a 4-years old plantation (Murcia, Spain). The fungal metaprofile was dominated by Ascomycota phylum. Desert truffle productivity was driven by different patterns of fungal species composition in soil (species replacement) and root (species richness differences). Moreover, positive associations for ectomycorrhizal and negative for arbuscular mycorrhizal guilds were found in productive roots, and positive associations for fungal parasite-plant pathogen guild in non-productive ones. Soil samples were dominated by pathotroph and saprotroph trophic modes, showing positive associations for Aureobasidium pullulans and Alternaria sp. in productive areas, and positive associations for Fusarium sp. and Mortierella sp. were found in non-productive soils. Finally, some significant OTUs were identified and associated to ascocarp producing patches, which could serve as predictive and location markers of desert truffle production.

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Advances in Desert Truffle Mycorrhization and Cultivation

Cited by 9 publications

References 33 publications

Growth promotion and mycorrhizal colonization of Argan (Argania spinosa (L.) Skeels) inoculated with the edible desert truffle Tirmania nivea (Desf.) Trappe

Growth promotion and mycorrhizal colonization of Argan (Argania spinosa (L.) Skeels) inoculated with the edible desert truffle Tirmania nivea (Desf.) Trappe

Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil

Different patterns in root and soil fungal diversity drive plant productivity of the desert truffle Terfezia claveryi in plantation

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