Advances in Drug Therapy for Peptic Ulcer Disease

Pappas, Theodore N.; Mulvihill, Sean J.; Goto, Yositi; Debas, Haile T.

doi:10.1001/archsurg.1987.01400160073011

Cited by 7 publications

(4 citation statements)

References 15 publications

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“…7. TNF-α was used as a positive control [1,[28][29][30]. It has been suggested that GGA stimulates the synthesis and secretion of mucin by cultured gastric mucussecreting cells without increasing their production of PGs.…”

Section: Discussionmentioning

confidence: 99%

Geranylgeranylacetone Induces Cyclooxygenase-2 Expression in Cultured Rat Gastric Epithelial Cells Through NF-κB

Nishida

Yabe

et al. 2007

Dig Dis Sci

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Geranylgeranylacetone (GGA) effectively protects the gastric mucosa against noxious agents. The precise mechanisms underlying the gastroprotective actions of GGA are not known. To elucidate the precise mechanism of GGA, the effect of GGA treatment on COX-2 expression in rat gastric epithelial (RGM1) cells was investigated. We used a prostaglandin E2 (PGE2) enzyme-linked immunoassay kit and Western blot analysis to measure PGE2 production and COX-2 induction by GGA treatment in serum-starved RGM1 cells. Gel-shift assay, Western blot analysis, and a reporter assay were performed to determine which COX-2 promoter was involved in GGA-induced COX-2 expression. GGA treatment dose dependently increased COX-2 expression and PGE2 production. The nuclear factor (NF)-kappaB sites of the COX-2 gene promoter were critical for GGA-mediated COX-2 expression. GGA induces COX-2 expression and increases PGE2 production in serum-starved RGM1 cells via activation of the NF-kappaB sites of COX-2 gene promoters.

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Section: Discussionmentioning

confidence: 99%

Geranylgeranylacetone Induces Cyclooxygenase-2 Expression in Cultured Rat Gastric Epithelial Cells Through NF-κB

Nishida

Yabe

et al. 2007

Dig Dis Sci

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“…[9][10][11] GGA is known to increase the synthesis and secretion of gastric mucin, 12,13 as well as the components of highmolecular-weight glycoproteins 14 and surface-active phospholipids. 15,16 Thus, GGA is considered to be a useful compound to study the mechanism by which pit cells up-regulate the synthesis of mucin.…”

Section: Introductionmentioning

confidence: 99%

Geranylgeranylacetone stimulates mucin synthesis in cultured guinea pig gastric pit cells by inducing a neuronal nitric oxide synthase

Rokutan

Teshima

Kawai

et al. 2000

Journal of Gastroenterology

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Nitric oxide (NO) has been considered to play an important role in the regulation of blood flow, mucosal integrity, and mucus production in the stomach. We investigated the stimulatory actions of epidermal growth factor (EGF) and a cytoprotective compound, geranylgeranylacetone (GGA), on mucin synthesis in guinea pig gastric pre-pit cells, maintained in a serum-free culture system. GGA increased [3H]glucosamine uptake and the accumulation of mucus granules positive for galactose oxidase-Schiff reaction in the cells. This stimulatory action of GGA was equivalent to that of EGF, but GGA did not stimulate the cell growth. Both EGF and GGA increased the release of NO degeneration products, NO2- and NO3-. The [3H]glucosamine uptake was completely inhibited by the non-selective NO synthase (NOS) inhibitors, N(G)-nitro-L-arginine and N(G)-monomethyl-L-arginine, and it was only partially inhibited by a more selective inhibitor for inducible NOS isoform (iNOS), aminoguanidine. Northern blotting with a cDNA probe for rat iNOS, and Western blotting with a polyclonal antibody against iNOS, demonstrated that GGA did not up-regulate the iNOS mRNA expression nor induce its protein. In contrast, GGA and EGF induced neuronal NOS, but not endothelial NOS, which was confirmed by immunoblot analyses with antibodies against these constitutive NOS isoforms. Thus, the present experiments suggests that GGA, as well as EGF, stimulates mucin synthesis at least in part through an NO-dependent pathway, leading to an increase in the integrity of the gastric mucosa.

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“…Geranylgeranylacetone (GGA), an isoprenoid compound in which retinoids are included, has been used orally as an anti-ulcer drug. This compound has been shown to be effective in protecting the gastric mucosa against several types of insults without affecting gastric acid secretion [13][14][15]. Hirakawa et al demonstrated that GGA induced HSPs in cultured guinea pig gastric mucosal cells and rat gastric mucosa [16].…”

Section: Introductionmentioning

confidence: 99%

An anti-ulcer drug, geranylgeranylacetone, suppresses inducible nitric oxide synthase in cultured vascular smooth muscle cells

Yamamoto

Sarukawa

Itō

et al. 2005

Journal of Hypertension

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Advances in Drug Therapy for Peptic Ulcer Disease

Cited by 7 publications

References 15 publications

Geranylgeranylacetone Induces Cyclooxygenase-2 Expression in Cultured Rat Gastric Epithelial Cells Through NF-κB

Geranylgeranylacetone Induces Cyclooxygenase-2 Expression in Cultured Rat Gastric Epithelial Cells Through NF-κB

Geranylgeranylacetone stimulates mucin synthesis in cultured guinea pig gastric pit cells by inducing a neuronal nitric oxide synthase

An anti-ulcer drug, geranylgeranylacetone, suppresses inducible nitric oxide synthase in cultured vascular smooth muscle cells

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