Advances in Folic Acid Biosensors and Their Significance in Maternal, Perinatal, and Paediatric Preventive Medicine

Movendane, Yogesh; Sipalo, Mbozu G.; Chan, Leon C. Z.

doi:10.3390/bios13100912

Cited by 2 publications

(1 citation statement)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, studies like those discussed above on the interaction of FAconju- It should be noted that the developed magnetic IC metrology could be useful for the investigation of many other functionalized magnetic nanoparticles, which are extensively studied, for example, for targeted hyperthermia treatments of cancer [44], the isolation and identification of circulating tumor cells [45], the diagnosis and treatment of cardiovascular diseases [46], and magnetic systems for personalized and precision medicine [47], etc. In addition, studies like those discussed above on the interaction of FAconjugated nanoparticles with other bio and chemical objects are of great interest for various applications, such as the development of targeted cancer contrast agents for MRI [48] or biosensors for preventive medicine [49], the adsorption of unfavorable chemical compounds and biological pollutants [50], and many others.…”

Section: Conjugation Of Mnp-sncl(dmf) With Mab-famentioning

confidence: 99%

Efficient Chlorostannate Modification of Magnetite Nanoparticles for Their Biofunctionalization

Zolotova,

Znoyko,

Orlov

et al. 2024

Materials

View full text Add to dashboard Cite

Magnetite nanoparticles (MNPs) are highly favored materials for a wide range of applications, from smart composite materials and biosensors to targeted drug delivery. These multifunctional applications typically require the biofunctional coating of MNPs that involves various conjugation techniques to form stable MNP–biomolecule complexes. In this study, a cost-effective method is developed for the chlorostannate modification of MNP surfaces that provides efficient one-step conjugation with biomolecules. The proposed method was validated using MNPs obtained via an optimized co-precipitation technique that included the use of degassed water, argon atmosphere, and the pre-filtering of FeCl2 and FeCl3 solutions followed by MNP surface modification using stannous chloride. The resulting chlorostannated nanoparticles were comprehensively characterized, and their efficiency was compared with both carboxylate-modified and unmodified MNPs. The biorecognition performance of MNPs was verified via magnetic immunochromatography. Mouse monoclonal antibodies to folic acid served as model biomolecules conjugated with the MNP to produce nanobioconjugates, while folic acid–gelatin conjugates were immobilized on the test lines of immunochromatography lateral flow test strips. The specific trapping of the obtained nanobioconjugates via antibody–antigen interactions was registered via the highly sensitive magnetic particle quantification technique. The developed chlorostannate modification of MNPs is a versatile, rapid, and convenient tool for creating multifunctional nanobioconjugates with applications that span in vitro diagnostics, magnetic separation, and potential in vivo uses.

show abstract

Section: Conjugation Of Mnp-sncl(dmf) With Mab-famentioning

confidence: 99%

Efficient Chlorostannate Modification of Magnetite Nanoparticles for Their Biofunctionalization

Zolotova,

Znoyko,

Orlov

et al. 2024

Materials

View full text Add to dashboard Cite

show abstract

Ultrasound-responsive nanocarriers with siRNA and Fe3O4 regulate macrophage polarization and phagocytosis for augmented non-small cell lung cancer immunotherapy

Li,

Zheng

et al. 2024

J Nanobiotechnol

View full text Add to dashboard Cite

The immunosuppressive tumor microenvironment (TME) significantly inhibits the effective anti-tumor immune response, greatly affecting the efficacy of immunotherapy. Most tumor-associated macrophages (TAMs) belong to the M2 phenotype, which contributes significantly to the immunosuppressive effects in non-small cell lung cancer (NSCLC) TME. The interaction between signal regulatory protein α (SIRPα) expressed on macrophages and CD47, a transmembrane protein overexpressed on cancer cells, activates the "eat-me-not" signaling pathway, inhibiting phagocytosis. In this study, a folic acid (FA)-modified ultrasound responsive gene/drugs delivery system, named FA@ PFP @ Fe 3 O 4 @LNB-SIRPα siRNA (FA-PFNB-SIRPα siRNA), was developed using 1,2-dioleoacyl-3-trimethylammonium-propane (DOTAP), FA-1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N- [amino (polyethylene glycol)2000] (DSPE-PEG2000-FA), cholesterol, and perfluoropentane (PFP), for the delivery of siRNA encoding SIRPα mRNA and immune adjuvant Fe 3 O 4 nanoparticles. Under ultrasound conditions, the nanobubbles effectively transfected macrophages, inhibiting SIRPα mRNA and protein expression, promoting the phagocytosis of TAMs, and synergistically reversing M2 polarization. This system promotes the infiltration of T cells, enhances the proliferation and activation of cytotoxic T cells, and inhibits the infiltration of immunosuppressive cells in tumor tissues. Administration of FA-PFNB-SIRPα siRNA combined with ultrasound significantly inhibits NSCLC progression. The study highlights the potential of ultrasound nanotechnology-enabled delivery of SIRPα siRNA and Fe 3 O 4 as an effective strategy for macrophage-based immunotherapy to reshape the immunosuppressive TME for cancer therapy. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1186/s12951-024-02883-w.

show abstract

Advances in Folic Acid Biosensors and Their Significance in Maternal, Perinatal, and Paediatric Preventive Medicine

Cited by 2 publications

References 110 publications

Efficient Chlorostannate Modification of Magnetite Nanoparticles for Their Biofunctionalization

Efficient Chlorostannate Modification of Magnetite Nanoparticles for Their Biofunctionalization

Ultrasound-responsive nanocarriers with siRNA and Fe3O4 regulate macrophage polarization and phagocytosis for augmented non-small cell lung cancer immunotherapy

Contact Info

Product

Resources

About