Advances in genetically engineered (transgenic) plants in pest management—an over view

Babu, R. Mohan; Sajeena, A.; Seetharaman, Koushik; Reddy, M. S.

doi:10.1016/s0261-2194(03)00142-x

Cited by 66 publications

(15 citation statements)

References 136 publications

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“…However, that cultivar contained a different insect-resistant gene than the cultivar used in our study. Comparing Cry1 with transgenic protease inhibitors in many studies, only the latter impacted the feeding behavior [50]–[53]. Better knowledge about the sublethal risks associated with ZMSJ and ZMKCKC pollen for honey bees may also be obtained studying the effects of pollen [13] or multiple Bt proteins [45] on larval development.…”

Section: Discussionmentioning

confidence: 99%

“…The hazard posed by Bt cotton pollen to B. mori primarily depends on the type of Cry gene present. The insecticidal crystal proteins (ICPs) that are encoded by the Cry1Ac and Cry2Ab genes have specific activity against certain lepidopterans larvae, and both have been used in many GM crops [8], [53]. However, the mechanisms that underlie the specificity of these genes remain unclear.…”

Section: Discussionmentioning

confidence: 99%

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Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Niu

Mannakkara

et al. 2013

PLoS ONE

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Transgenic insect-resistant cotton (Bt cotton) has been extensively planted in China, but its effects on non-targeted insect species such as the economically important honey bee (Apis mellifera) and silkworm (Bombyx mori) currently are unknown. In this study, pollen from two Bt cotton cultivars, one expressing Cry1Ac/EPSPS and the other expressing Cry1Ac/Cry2Ab, were used to evaluate the effects of Bt cotton on adult honey bees and silkworm larvae. Laboratory feeding studies showed no adverse effects on the survival, cumulative consumption, and total hemocyte count (THC) of A. mellifera fed with Bt pollen for 7 days. No effects on the survival or development of B. mori larvae were observed either. A marginally significant difference between Cry1Ac/Cry2Ab cotton and the conventional cotton on the THC of the 3rd day of 5th B. mori instar larvae was observed only at the two highest pollen densities (approximately 900 and 8000 grains/cm2), which are much higher than the pollen deposition that occurs under normal field conditions. The results of this study show that pollen of the tested Bt cotton varieties carried no lethal or sublethal risk for A. mellifera, and the risk for B. mori was negligible.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Niu

Mannakkara

et al. 2013

PLoS ONE

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“…The generation of transgenic plants expressing foreign PIs is a strategy that continues to be investigated for pest control (Hilder et al . 1987; Mohan Babu et al . 2003).…”

Section: Introductionmentioning

confidence: 99%

Impact of oilseed rape expressing the insecticidal serine protease inhibitor, mustard trypsin inhibitor‐2 on the beneficial predator Pterostichus madidus

et al. 2004

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Insect-resistant transgenic plants have been suggested to have deleterious effects on beneficial predators feeding on crop pests, through transmission of the transgene product by the pest to the predator. To test this hypothesis, effects of oilseed rape expressing the serine protease inhibitor, mustard trypsin inhibitor -2 (MTI-2), on the predatory ground beetle Pterostichus madidus were investigated, using diamondback moth, Plutella xylostella as the intermediary pest species. As expected, oilseed rape expressing MTI-2 had a deleterious effect on the development and survival of the pest. However, incomplete pest mortality resulted in survivors being available to predators at the next trophic level, and inhibition studies confirmed the presence of biologically active transgene product in pest larvae. Characterization of proteolytic digestive enzymes of P. madidus demonstrated that adults utilize serine proteases with trypsin-like and chymotrypsin-like specificities; the former activity was completely inhibited by MTI-2 in vitro. When P. madidus consumed prey reared on MTI-2 expressing plants over the reproductive period in their life cycle, no significant effects upon survival were observed as a result of exposure to the inhibitor. However, there was a short-term significant inhibition of weight gain in female beetles fed unlimited prey containing MTI-2, with a concomitant reduction of prey consumption. Biochemical analyses showed that the inhibitory effects of MTI-2 delivered via prey on gut proteolysis in the carabid decreased with time of exposure, possibly resulting from up-regulation of inhibitor-insensitive proteases. Of ecological significance, consumption of MTI-2 dosed prey had no detrimental effects on reproductive fitness of adult P. madidus.

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“…Of the several biological functions of plant lectins [ 30 ], their anti-insect activities have received particular attention [ 17 , 31 ]. Over the last few years, the development of plants resistant to insect attacks has opened a promising field for the use of plant lectins in pest management strategies [ 32 , 33 ]. The use of lectins in transgenic plants has yielded positive results, especially for crops now expressing Cry toxins from Bacillus thuringiensis ( Bt ), which demonstrate resistance to sap-sucking insects.…”

Section: Anti-insect Activity and Mode Of Action Of Plant Lectinsmentioning

confidence: 99%

Insecticidal Activity of Plant Lectins and Potential Application in Crop Protection

2015

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Lectins constitute a complex group of proteins found in different organisms. These proteins constitute an important field for research, as their structural diversity and affinity for several carbohydrates makes them suitable for numerous biological applications. This review addresses the classification and insecticidal activities of plant lectins, providing an overview of the applicability of these proteins in crop protection. The likely target sites in insect tissues, the mode of action of these proteins, as well as the use of lectins as biotechnological tools for pest control are also described. The use of initial bioassays employing artificial diets has led to the most recent advances in this field, such as plant breeding and the construction of fusion proteins, using lectins for targeting the delivery of toxins and to potentiate expected insecticide effects. Based on the data presented, we emphasize the contribution that plant lectins may make as tools for the development of integrated insect pest control strategies.

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Advances in genetically engineered (transgenic) plants in pest management—an over view

Cited by 66 publications

References 136 publications

Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Impact of oilseed rape expressing the insecticidal serine protease inhibitor, mustard trypsin inhibitor‐2 on the beneficial predator Pterostichus madidus

Insecticidal Activity of Plant Lectins and Potential Application in Crop Protection

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