Advances in glucosamine production from waste biomass and microbial fermentation technology and its applications

Ahuja, Vishal; Bhatt, Arvind Kumar; Vp, Sharma; Rathour, Ranju Kumari; Rana, Nidhi; Bhatia, Rajendra; Varjani, Sunita; Kumar, Manu; Magdouli, Sara; Yung-HunYang,; Bhatia, Shashi Kant

doi:10.1007/s13399-021-01968-y

Cited by 15 publications

(7 citation statements)

References 132 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…35 Despite the high demand for glucosamine, its production through environment-friendly methods remains a challenge. 10 A promising option in this regard is the use of N-acetylglucosamine deacetylases, such as CmCBDA, to produce GlcN from GlcNAc. Here, we elucidated the catalytic mechanism of CmCBDA, and we performed structure-based enzyme engineering to enhance its catalytic activity.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Glucosamine is thus currently allowed for use as a dietary supplement in the United States . Despite the high demand for glucosamine, its production through environment-friendly methods remains a challenge . A promising option in this regard is the use of N -acetylglucosamine deacetylases, such as CmCBDA, to produce GlcN from GlcNAc.…”

Section: Discussionmentioning

confidence: 99%

“…Industrially, GlcN is mainly produced via the acid hydrolysis of chitin from shrimp and crab shells. , The process involves the hydrolysis of raw materials to GlcNAc, followed by the deacetylation of this intermediate . Recently, a novel fermentation-based technology was developed for cost-effective production of high-quality GlcN and GlcNAc , through metabolic engineering of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Structural Insights into the Catalytic Activity of Cyclobacterium marinum N-Acetylglucosamine Deacetylase

Hu,

Xu,

Xie

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

N-Acetylglucosamine deacetylase from Cyclobacterium marinum (CmCBDA) is a highly effective and selective biocatalyst for the production of D-glucosamine (GlcN) from N-acetylglucosamine (GlcNAc). However, the underlying catalytic mechanism remains elusive. Here, we show that CmCBDA is a metalloenzyme with a preference for Ni 2+ over Mn 2+ . Crystal structures of CmCBDA in complex with Ni 2+ and Mn 2+ revealed slight remodeling of the CmCBDA active site by the metal ions. We also demonstrate that CmCBDA exists as a mixture of homodimers and monomers in solution, and dimerization is indispensable for catalytic activity. A mutagenesis analysis also indicated that the active site residues Asp22, His72, and His143 as well as the residues involved in dimerization, Pro52, Trp53, and Tyr55, are essential for catalytic activity. Furthermore, a mutation on the protein surface, Lys219Glu, resulted in a 2.3-fold improvement in the deacetylation activity toward GlcNAc. Mechanistic insights obtained here may facilitate the development of CmCBDA variants with higher activities.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structural Insights into the Catalytic Activity of Cyclobacterium marinum N-Acetylglucosamine Deacetylase

Hu,

Xu,

Xie

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…GlcNAc has been studied for its use in medicine, such as treating osteoarthritis, restoring and maintaining cartilage, reducing inflammation, and protecting the liver (Ahuja et al 2021). In food processing, the application of GlcNAc is still limited, although several applications have been reported, including supplementation of GlcNAc in food products, as a growth promotor for probiotics in fermented milk (Chen et al 2004) and as a sweetener in food and beverages (Wassenaar, 2004).…”

Section: N-acetylglucosaminementioning

confidence: 99%

Bioproduction of Chitin Hydrolysate Containing N-Acetylglucosamine by Serratia marcescens PT6 Crude Chitinase and Its Effects on Bacterial Growth Inhibition in Various Temperature

Puspita

Ratnawati

Setiawan

et al. 2023

ASEAN J. Chem. Eng.

View full text Add to dashboard Cite

N-acetylglucosamine (GlcNAc), a chitin monomer, can be used as a natural preservative to ensure food quality and safety. Combining natural preservatives with low storage temperature offers physical hurdles to bacterial growth in food. This study aimed to produce chitin hydrolysate containing GlcNAc using Serratia marcescens PT6 crude chitinase and investigate its effect on bacterial growth rate as a function of temperature. Crude chitinase from partial purification was used to hydrolyze 1.3% colloidal chitin. The optimal enzymatic conditions were pH 6 and 45˚C for 120 min, at an enzyme:substrate ratio of 1:1, yielding a 65.6 µg/mL GlcNAc. Inhibitory activity of hydrolysate containing 2.5-7.5 ppm GlcNAc on Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Vibrio parahaemolyticus was measured at 4, 15, and 30oC in nutrient broth. Bacterial growth was measured using of optical density for each combination of GlcNAc concentration and temperature. Growth curves fitted by the Baranyi and Roberts model were developed using DMFit software. The growth rate was converted to the square root and then modeled as a function of temperature using the Ratkowsky square root model. Incubation temperature exerted a pronounced effect on the inhibition of all bacterial species (P<0.0001), with the greatest effect observed for E. coli at 30°C (P<0.0001), and the least effect for V. parahaemolyticus (P=0.0878). The inhibitory effect of GlcNAc in chitin hydrolysate was only significant for E. coli (P<0.0001) and S. aureus (P=0.0041). This study revealed that the effect of temperature in growth inhibition was more significant than GlcNAc addition. However, a reduction in bacterial growth with the addition of GlcNAc at 30°C was observed, which may be effective for food encountered thermal abuse conditions. Further investigation of the effect of GlcNAc on bacteria structure and metabolism is required to elucidate the mechanism of GlcNAc as a food preservative.

show abstract

“…Nevertheless, the reuse of waste from the industry is not a common practice, and a significant proportion of crustacean waste is deposited into the environment, generating potential environmental hazards due to fish tissue deterioration. Importantly, crustacean shells contain chitin, proteins, calcium carbonate, and carotenoids, which are valuable resources for pharmaceutical, agricultural, construction, and paper industries [ 1 , 2 , 3 , 4 , 5 ]. For example, treatments with chitin and shell derivatives have been shown to increase soil suppressiveness against plant fungal and nematode infections [ 6 , 7 , 8 , 9 , 10 ] and therefore attract significant attention as potential environmentally friendly approaches to control plant pathogens.…”

Section: Introductionmentioning

confidence: 99%

Microbial Consortium Associated with Crustacean Shells Composting

2022

View full text Add to dashboard Cite

Soil microbes play an essential role in the biodegradation of crustacean shells, which is the process of sustainable bioconversion to chitin derivatives ultimately resulting in the promotion of plant growth properties. While a number of microorganisms with chitinolytic properties have been characterized, little is known about the microbial taxa that participate in this process either by active chitin degradation or by facilitation of this activity through nutritional cooperation and composting with the chitinolytic microorganisms. In this study, we evaluated the transformation of the soil microbiome triggered by close approximation to the green crab shell surface. Our data indicate that the microbial community associated with green crab shell matter undergoes significant specialized changes, which was reflected in a decreased fungal and bacterial Shannon diversity and evenness and in a dramatic alteration in the community composition. The relative abundance of several bacterial and fungal genera including bacteria Flavobacterium, Clostridium, Pseudomonas, and Sanguibacter and fungi Mortierella, Mycochlamys, and Talaromyces were increased with approximation to the shell surface. Association with the shell triggered significant changes in microbial cooperation that incorporate microorganisms that were previously reported to be involved in chitin degradation as well as ones with no reported chitinolytic activity. Our study indicates that the biodegradation of crab shells in soil incorporates a consortium of microorganisms that might provide a more efficient way for bioconversion.

show abstract

Advances in glucosamine production from waste biomass and microbial fermentation technology and its applications

Cited by 15 publications

References 132 publications

Structural Insights into the Catalytic Activity of Cyclobacterium marinum N-Acetylglucosamine Deacetylase

Structural Insights into the Catalytic Activity of Cyclobacterium marinum N-Acetylglucosamine Deacetylase

Bioproduction of Chitin Hydrolysate Containing N-Acetylglucosamine by Serratia marcescens PT6 Crude Chitinase and Its Effects on Bacterial Growth Inhibition in Various Temperature

Microbial Consortium Associated with Crustacean Shells Composting

Contact Info

Product

Resources

About