Advances in in situ and in-line liquid–liquid extraction for bioprocess intensification

Abstract

Liquid-liquid extraction (LLE) has been commonly used to purify products downstream in bioprocess. LLE can be operated in different modes. We specifically focus on two modes: (1) in-situ and (2)...

A multistep (semi)-continuous biocatalytic setup for the production of polycaprolactone

“…Moreover, it is also possible to achieve and uphold the optimal process conditions for each step in the system and to avoid the need for isolation of intermediates, thereby reducing errors and losses along the production line. 22,24–27 Utilizing reactors with inner dimensions in the mm range for these applications also allows for improved heat and mass transfer and better control of the different unit operations, resulting in more reliable processes. 28 In this work, we aim to present a novel approach for a three-step, semi-continuous production of PCL to show the advantages that continuous manufacturing offers to increase the speed and productivity of biocatalytic processes.…”

Integrated Continuous-Flow Production of Wax Esters Combining Whole-Cell and In Vitro Biocatalysis

“…The in-line separation can faciliate the process integration . The two immiscible liquid phases from the bioconversion could be separated by an in-line membrane-based liquid–liquid separator.…”

“…The in-line separation can faciliate the process integration. 13 The two immiscible liquid phases from the bioconversion could be separated by an in-line membrane-based liquid− liquid separator. To prevent large water content in the second reactor, where the esterification process was carried out, the aqueous phase was separated.…”